Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CEP76_RS07300 Genome accession   NZ_CP022037
Coordinates   1496026..1497420 (-) Length   464 a.a.
NCBI ID   WP_010945036.1    Uniprot ID   Q7VM73
Organism   [Haemophilus] ducreyi strain FDAARGOS_297     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1491026..1502420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEP76_RS07270 (CEP76_07275) lptE 1491769..1492266 (+) 498 WP_010945042.1 LPS assembly lipoprotein LptE -
  CEP76_RS07275 (CEP76_07280) holA 1492272..1493297 (+) 1026 WP_010945041.1 DNA polymerase III subunit delta -
  CEP76_RS07280 (CEP76_07285) yacG 1493331..1493519 (-) 189 WP_010945040.1 DNA gyrase inhibitor YacG -
  CEP76_RS07285 (CEP76_07290) coaE 1493522..1494139 (-) 618 WP_010945039.1 dephospho-CoA kinase -
  CEP76_RS07290 (CEP76_07295) - 1494157..1494849 (-) 693 WP_041603480.1 prepilin peptidase -
  CEP76_RS07295 (CEP76_07300) pilC 1494849..1496042 (-) 1194 WP_010945037.1 type II secretion system F family protein Machinery gene
  CEP76_RS07300 (CEP76_07305) pilB 1496026..1497420 (-) 1395 WP_010945036.1 GspE/PulE family protein Machinery gene
  CEP76_RS07305 (CEP76_07310) pilA 1497432..1497881 (-) 450 WP_010945035.1 prepilin peptidase-dependent pilin Machinery gene
  CEP76_RS07310 (CEP76_07315) radA 1498075..1499454 (+) 1380 WP_010945034.1 DNA repair protein RadA -
  CEP76_RS07315 (CEP76_07320) - 1499521..1499736 (-) 216 WP_010945033.1 YgjV family protein -
  CEP76_RS07320 (CEP76_07325) - 1499858..1500343 (-) 486 WP_010945031.1 dihydrofolate reductase -
  CEP76_RS07325 (CEP76_07330) - 1500462..1501211 (+) 750 WP_041603673.1 TSUP family transporter -
  CEP76_RS07330 (CEP76_07335) nth 1501241..1501876 (+) 636 WP_010945029.1 endonuclease III -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52913.08 Da        Isoelectric Point: 6.6454

>NTDB_id=236056 CEP76_RS07300 WP_010945036.1 1496026..1497420(-) (pilB) [[Haemophilus] ducreyi strain FDAARGOS_297]
MQYAVFDLHRQQNFIISAERWQKNCQEKDLLLRYLALPVQENEDRLWLAIDDMKNLNACEIFAFMAHKTIEPVLVSAEEL
KYLLNQLAPEQQAVYEESELTYQVQTESETLNLNDPIIQLLDNLFKFCLRQNASDIHFEPQKAQLQIRLRIDGVLHIYKT
LAIHLAARIISRIKLLARLDISETRLPQDGQFHFKTIVSETLDFRVSSLPTQWGGKIVLRLQKNKPTHFDFVELGLLPAQ
KTTLLHLLHQPQGLILVTGPTGSGKSITLYSALSHLNQPDKHILTAEDPIEIEIEGIIQTQVNRAIQLDFSHLLRTFLRQ
DPDIIMLGEIRDPESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYELKNALLLVIAQRLVRKLCTQCFGKGC
QACYQGYRGRIGIYQLLSRTGKIFEKETAYLDFASLHHSAKHKLEADITSLAEINRVLGDAENI

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=236056 CEP76_RS07300 WP_010945036.1 1496026..1497420(-) (pilB) [[Haemophilus] ducreyi strain FDAARGOS_297]
ATGCAGTATGCGGTATTTGATCTTCATCGCCAACAGAATTTTATTATTTCAGCGGAGAGATGGCAAAAGAACTGTCAAGA
AAAAGATCTTTTATTACGTTATCTTGCATTACCGGTTCAAGAAAATGAGGACAGGTTATGGCTAGCCATTGATGATATGA
AAAATTTAAATGCGTGTGAAATTTTTGCATTTATGGCTCATAAAACGATAGAACCGGTGTTGGTTTCTGCGGAAGAATTG
AAATATTTATTGAATCAACTGGCGCCAGAGCAACAAGCGGTATATGAAGAAAGTGAGCTAACTTATCAAGTACAAACAGA
AAGTGAAACATTGAATTTAAATGATCCTATTATTCAATTACTTGATAATTTATTTAAATTTTGTTTAAGGCAAAATGCTT
CCGATATTCATTTTGAGCCACAAAAAGCACAGTTACAGATTCGGTTGCGGATTGATGGTGTGTTACATATCTACAAAACA
TTGGCAATTCATTTGGCTGCTCGCATTATTTCACGTATCAAATTGTTGGCTAGACTTGATATTAGCGAAACACGGCTACC
GCAAGATGGGCAATTCCATTTTAAAACAATCGTATCTGAAACCCTTGATTTCCGTGTATCGAGTTTGCCTACTCAATGGG
GCGGAAAAATTGTATTACGCTTACAAAAAAATAAACCGACCCATTTTGATTTTGTTGAATTGGGGTTATTGCCTGCGCAA
AAAACTACGTTATTACACTTATTGCATCAACCACAAGGTCTAATTTTAGTTACTGGCCCCACCGGCAGTGGCAAAAGTAT
CACATTGTATAGTGCATTAAGTCATCTGAATCAACCGGATAAGCATATTTTAACGGCAGAAGATCCAATAGAAATTGAGA
TCGAAGGAATCATTCAAACACAAGTTAATCGAGCGATTCAGCTAGATTTTAGCCATCTATTACGAACATTTTTGCGCCAA
GATCCTGATATTATTATGTTAGGTGAGATTCGTGATCCGGAGAGTGCCGAAATTGCATTACGCGCTGCACAAACAGGGCA
TCTTGTGCTTTCTACATTACATACTAATGATGCGCCCTCTGCAATTGAACGGCTATTACAATTAGGTATTAAAGAATATG
AGCTTAAAAACGCCTTATTATTAGTCATTGCACAACGGCTTGTCCGCAAGCTATGCACGCAATGTTTTGGTAAAGGCTGT
CAGGCTTGCTATCAGGGCTATAGAGGACGCATTGGCATTTATCAATTATTAAGCCGTACGGGAAAAATCTTTGAAAAAGA
GACCGCTTACCTTGATTTTGCTAGTTTACATCATAGTGCGAAGCATAAGTTAGAGGCTGATATTACGTCCTTAGCAGAAA
TTAACAGAGTATTAGGCGATGCTGAAAATATATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-92)

Type II/IV secretion system protein (T2SSE)

(129-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q7VM73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

74.017

98.707

0.731
  pilB Haemophilus influenzae Rd KW20

58.482

96.552

0.565
  pilB Haemophilus influenzae 86-028NP

57.906

96.767

0.56
  pilB Vibrio campbellii strain DS40M4

40.408

100

0.427
  pilB Vibrio cholerae strain A1552

39.919

100

0.422
  pilB Vibrio parahaemolyticus RIMD 2210633

38.386

100

0.42
  pilB Legionella pneumophila strain ERS1305867

38.618

100

0.409
  pilB Acinetobacter baylyi ADP1

38.493

100

0.407
  pilB Acinetobacter baumannii D1279779

37.654

100

0.394
  pilF Neisseria gonorrhoeae MS11

36.076

100

0.369

Multiple sequence alignment