Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O23A_RS15560 Genome accession   NZ_CP021654
Coordinates   3419519..3421225 (+) Length   568 a.a.
NCBI ID   WP_087756646.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain O23A     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3414519..3426225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O23A_RS15535 (O23A_p3214) pdhR 3415641..3416405 (-) 765 WP_005314150.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  O23A_RS15540 (O23A_p3216) ampD 3416682..3417254 (-) 573 WP_087756643.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  O23A_RS15545 (O23A_p3217) - 3417404..3417874 (+) 471 WP_198315355.1 TIGR02281 family clan AA aspartic protease -
  O23A_RS15550 (O23A_p3218) nadC 3417879..3418736 (+) 858 WP_087756645.1 carboxylating nicotinate-nucleotide diphosphorylase -
  O23A_RS15555 (O23A_p3219) - 3419072..3419512 (+) 441 WP_087757417.1 pilin -
  O23A_RS15560 (O23A_p3220) pilB 3419519..3421225 (+) 1707 WP_087756646.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  O23A_RS15565 (O23A_p3221) pilC 3421358..3422599 (+) 1242 WP_059113460.1 type II secretion system F family protein Machinery gene
  O23A_RS15570 (O23A_p3222) pilD 3422663..3423538 (+) 876 WP_087756647.1 prepilin peptidase Machinery gene
  O23A_RS15575 (O23A_p3223) coaE 3423558..3424172 (+) 615 WP_087756648.1 dephospho-CoA kinase -
  O23A_RS15580 (O23A_p3224) zapD 3424210..3424932 (+) 723 WP_021140336.1 cell division protein ZapD -
  O23A_RS15585 (O23A_p3225) yacG 3424942..3425136 (+) 195 WP_005314167.1 DNA gyrase inhibitor YacG -
  O23A_RS15590 (O23A_p3226) mutT 3425214..3425621 (-) 408 WP_005314168.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62561.00 Da        Isoelectric Point: 5.4384

>NTDB_id=232297 O23A_RS15560 WP_087756646.1 3419519..3421225(+) (pilB) [Aeromonas salmonicida strain O23A]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESEQDALGMEEIDESEI
SELEVSDENSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=232297 O23A_RS15560 WP_087756646.1 3419519..3421225(+) (pilB) [Aeromonas salmonicida strain O23A]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCATCCCTGCTCAGTGAGTCCGACTCACAGCGTTA
CCTGAGCCAAGCCAAGGCCCAGCGCAAACCATTCGTGACATTTCTGATTGAAAACGAGATCCTCGACAGCAAGGCGCTGG
CGGATTTTTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTAGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGACTCCATACCGAAGCCCTGCTGGTGGAAGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAAAGCGAGCAGGATGCCCTCGGCATGGAGGAGATCGACGAGTCGGAGATC
TCCGAGCTGGAAGTCTCAGACGAAAACTCCCGCCTCGATGAGAGCGTCAATACCGCCGACGACGATGCCCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGCGCCTCGGATCTGCACTTCGAACCCTATGAGACCAAGTACC
GTATCCGTTTTCGGATCGATGGCATACTGCACGAGATAGCGACGCCGCCGGTCAATCTGGCCAACCGCTTTTCGGCCCGC
CTCAAGGTGATGGCGCGACTCGATATCGCAGAGCGACGGCTGCCCCAGGATGGTCGCATCAAGTTAAAGCTGTCGCGCAA
CAAATCCATGGATATGCGGGTCAACACACTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTGGACTCTTCCG
CAGCGCGGCTCAATATCGAACAGCTCGGCTTCGACGACCGGCAGAAAGCACAGTATCTGCGTGCCCTCTCCAAGCCCCAG
GGCATGATACTGGTGACGGGCCCGACCGGCTCTGGCAAGACGGTCTCTCTCTATACCGGCCTCAATATTCTCAACACCAC
AGAGGTGAACATCTCCACTGCCGAAGATCCGGTGGAGATCAACCTGCCAGGCGTCAACCAGGTACAAGTCAATCCCAAGG
CGGGGTTGACCTTCGCCAGCGCCCTGCGATCCTTTCTTCGCCAGGATCCCGACGTTGTGATGGTGGGGGAAATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAAGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCTGC
CGAAACCCTGACCCGGATGATGAACATGGGGGTCCCGGCCTTCAATATCGCCTCTTCGGTGACCCTCATCATGGCCCAGC
GCCTCGCTCGCAAGCTCTGCGATCACTGCAAGGCACCGGAAGTGGTGCCCGAAGCTGAATTGCTGGAGCTGGGCTTCACC
CAGCAGCAGTTGGCCGCCGGCTTGCGCCTGTTCAAGCCAGTAGGCTGCAAAGAGTGCTCGGGGGGCTACAAGGGTCGGGT
CGGTATCTACGAAATCATGCTAATGTCTGAAAACATTGCAAAATTGATCATGCAAGGAGCCAACTCCCTGCAAATTGCAG
CGATTGCTCAAAAAGAGGGGATGCGAACCCTGCGTATCTCGGGCCTGGAGAAGGCCCGCCTGGGGGTGACCAGCCTGGCA
GAGATCAACCGGGTGACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.614

100

0.558
  pilB Acinetobacter baumannii D1279779

55.556

99.824

0.555
  pilB Legionella pneumophila strain ERS1305867

54.594

99.648

0.544
  pilB Vibrio cholerae strain A1552

54.513

99.472

0.542
  pilB Vibrio campbellii strain DS40M4

52.12

99.648

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.341

92.958

0.375

Multiple sequence alignment