Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   CCO03_RS18975 Genome accession   NZ_CP021455
Coordinates   4434712..4435239 (-) Length   175 a.a.
NCBI ID   WP_087283639.1    Uniprot ID   A0A1Y0ESB1
Organism   Comamonas serinivorans strain DSM 26136     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4429712..4440239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCO03_RS20000 - 4430177..4431100 (+) 924 WP_157667804.1 hypothetical protein -
  CCO03_RS18955 (CCO03_18955) fic 4431392..4432522 (-) 1131 WP_087283632.1 protein adenylyltransferase Fic -
  CCO03_RS18960 (CCO03_18960) - 4432677..4432937 (+) 261 WP_087283634.1 hypothetical protein -
  CCO03_RS18965 (CCO03_18965) - 4433252..4434022 (-) 771 WP_157667805.1 pilus assembly protein -
  CCO03_RS18970 (CCO03_18970) - 4434092..4434586 (-) 495 WP_269466884.1 pilin -
  CCO03_RS18975 (CCO03_18975) pilE 4434712..4435239 (-) 528 WP_087283639.1 pilin Machinery gene
  CCO03_RS18980 (CCO03_18980) - 4435646..4436389 (-) 744 WP_236903943.1 putative toxin-antitoxin system toxin component, PIN family -
  CCO03_RS18985 (CCO03_18985) - 4436379..4437773 (-) 1395 WP_087283641.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  CCO03_RS18990 (CCO03_18990) - 4437948..4438547 (-) 600 WP_087283644.1 hypothetical protein -
  CCO03_RS18995 (CCO03_18995) - 4438684..4439319 (+) 636 WP_087283646.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 175 a.a.        Molecular weight: 17761.49 Da        Isoelectric Point: 4.8010

>NTDB_id=230878 CCO03_RS18975 WP_087283639.1 4434712..4435239(-) (pilE) [Comamonas serinivorans strain DSM 26136]
MKKIQQGFTLIELMIVVAIIGILAAIALPAYQDYTVRSKVSEMVIAATSPKSLISEAFQSDGLAGVTAAVAEYNARPAAE
KASKFLDPTTPIVIGAAGDITVMSGTVADAGLPTEAAGKTLVFTPNVQGAALVAGVTGAIDWSCASTANTTATTRGLVVA
ANGDMPAKYAPSECR

Nucleotide


Download         Length: 528 bp        

>NTDB_id=230878 CCO03_RS18975 WP_087283639.1 4434712..4435239(-) (pilE) [Comamonas serinivorans strain DSM 26136]
ATGAAAAAGATTCAACAAGGCTTCACGCTGATCGAACTGATGATCGTTGTGGCGATCATCGGCATCCTGGCTGCCATCGC
GCTGCCGGCTTATCAGGATTACACCGTGCGTTCGAAGGTGTCGGAAATGGTGATCGCCGCCACTTCGCCCAAGAGCTTGA
TCAGCGAAGCGTTCCAATCGGATGGCTTGGCCGGCGTTACCGCTGCCGTCGCAGAATATAACGCTCGTCCTGCAGCTGAA
AAAGCCAGCAAATTTTTGGATCCGACTACTCCTATCGTGATTGGCGCAGCTGGCGACATCACCGTGATGTCGGGTACCGT
CGCTGATGCTGGTCTGCCAACTGAAGCAGCAGGTAAGACTTTGGTTTTCACGCCTAATGTGCAAGGTGCTGCTCTCGTTG
CGGGTGTGACCGGTGCTATCGACTGGTCTTGCGCATCTACCGCCAACACCACTGCCACGACGCGTGGCTTGGTTGTGGCT
GCTAACGGCGACATGCCCGCCAAGTACGCGCCTTCCGAATGCCGTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-175)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y0ESB1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

52.85

100

0.583
  pilA Ralstonia pseudosolanacearum GMI1000

41.209

100

0.429
  pilA2 Legionella pneumophila str. Paris

39.205

100

0.394
  pilA2 Legionella pneumophila strain ERS1305867

38.636

100

0.389
  comP Acinetobacter baylyi ADP1

38.286

100

0.383
  pilA/pilA1 Eikenella corrodens VA1

40

91.429

0.366

Multiple sequence alignment