Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OLMES_RS09475 Genome accession   NZ_CP021425
Coordinates   2161681..2163390 (-) Length   569 a.a.
NCBI ID   WP_198343281.1    Uniprot ID   A0A1Y0I693
Organism   Oleiphilus messinensis strain ME102     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2156681..2168390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OLMES_RS09440 (OLMES_1921) - 2156886..2157623 (-) 738 WP_087461035.1 LrgB family protein -
  OLMES_RS09445 (OLMES_1922) - 2157628..2158005 (-) 378 WP_087461036.1 CidA/LrgA family protein -
  OLMES_RS09450 (OLMES_1923) - 2158002..2158490 (-) 489 WP_087461037.1 HIT family protein -
  OLMES_RS09455 (OLMES_1924) - 2158517..2158741 (-) 225 WP_087461038.1 DNA gyrase inhibitor YacG -
  OLMES_RS09460 (OLMES_1925) coaE 2158810..2159436 (-) 627 WP_087461039.1 dephospho-CoA kinase -
  OLMES_RS09465 (OLMES_1926) pilD 2159512..2160381 (-) 870 WP_087461040.1 A24 family peptidase Machinery gene
  OLMES_RS09470 (OLMES_1927) pilC 2160458..2161678 (-) 1221 WP_087461041.1 type II secretion system F family protein Machinery gene
  OLMES_RS09475 (OLMES_1928) pilB 2161681..2163390 (-) 1710 WP_198343281.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OLMES_RS09480 (OLMES_1929) - 2163746..2164192 (+) 447 WP_087461043.1 pilin -
  OLMES_RS09485 (OLMES_1930) - 2164280..2166136 (+) 1857 WP_087461044.1 hypothetical protein -
  OLMES_RS09490 (OLMES_1931) - 2166102..2167049 (+) 948 WP_087461045.1 glycosyltransferase family 2 protein -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62639.70 Da        Isoelectric Point: 5.1575

>NTDB_id=230585 OLMES_RS09475 WP_198343281.1 2161681..2163390(-) (pilB) [Oleiphilus messinensis strain ME102]
MSNSANIVLTGLARRFVDDGILDEASAKDAFVQASNNKIQFITFLVQNSLAEARPLATSAGHEFGMPVFDLESVLTEVTP
EKLVEEKIIRKHKALPLFKRGTRLFIAVSDPTNIQAIDEIRFHTGLSTEAVLVEDDKLTSAIERFLEAQDTTMSNLDDAD
LDGVELDEGNDDDQDVTKSDADDAPIVKFCNKMLLDAIKGGSSDIHFEPYEKAYRVRYRTDGILHEVSRPNVKLANKIAA
RMKVMSQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSSAKMGIDALGYEEVQKQMYLTALHQP
QGMILVTGPTGSGKTVSLYTGLNILNTPEVNISTAEDPVEINLEGINQVNVSTKVGLGFAEALRAFLRQDPDVVMVGEIR
DLETASIAIKAAQTGHMVLSTLHTNSAPETLTRMLNMGVPAFNIATSVSLIIAQRLARRLCSNCKEKADIPKETLLKEGF
LEEHFTPDFALYKPKGCEKCNDGYKGRVGIYEVVRVTPSISQLIMSEASSLEIAAEAQKCGFNNLRQSAILKAVQGLTSL
EEVNRVTKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=230585 OLMES_RS09475 WP_198343281.1 2161681..2163390(-) (pilB) [Oleiphilus messinensis strain ME102]
ATGTCCAATTCTGCCAATATCGTGTTAACCGGTCTGGCCAGGCGATTTGTTGATGACGGTATTCTGGATGAAGCGTCCGC
AAAAGACGCCTTCGTCCAGGCCAGCAACAACAAAATCCAGTTTATCACTTTTCTTGTACAAAACTCCCTGGCAGAGGCTC
GTCCGCTAGCAACCTCGGCCGGCCACGAATTCGGTATGCCTGTTTTTGATCTGGAAAGCGTTCTGACGGAGGTGACGCCA
GAAAAACTGGTTGAAGAAAAAATCATTCGCAAACACAAAGCGTTGCCTTTATTTAAGAGAGGCACCCGCTTGTTCATTGC
TGTATCCGACCCTACTAATATCCAAGCAATTGATGAAATTCGCTTTCACACCGGATTAAGCACTGAAGCGGTACTGGTCG
AAGATGACAAGTTGACCAGCGCCATTGAGCGATTCCTCGAAGCCCAAGATACCACCATGTCAAATCTTGATGATGCAGAC
TTGGACGGTGTTGAGCTTGATGAGGGGAATGACGATGATCAAGATGTTACCAAGTCAGACGCAGATGATGCCCCAATTGT
TAAGTTCTGCAACAAGATGTTGCTTGACGCGATCAAAGGCGGCTCATCAGATATTCACTTCGAACCTTATGAAAAGGCTT
ATCGGGTTCGATACCGCACAGATGGCATTTTGCATGAGGTTTCCAGACCTAATGTAAAGCTAGCCAATAAAATTGCAGCG
CGTATGAAAGTTATGTCCCAACTGGATATTTCAGAGCGACGGGTTCCGCAAGATGGTCGGATTAAGATGAAACTCTCCAA
GACGAAAGCCATCGACTTCAGGGTGAATACTCTGCCGACACTGTGGGGTGAAAAAATCGTTCTCCGGATACTGGATCCAA
GCAGTGCCAAAATGGGAATTGATGCATTGGGCTATGAAGAGGTACAGAAACAAATGTACCTTACCGCTCTGCATCAACCT
CAAGGCATGATTCTGGTCACCGGCCCCACAGGGAGTGGCAAAACCGTCTCGCTCTACACCGGGCTCAATATTCTAAATAC
CCCCGAGGTAAATATTTCTACCGCAGAAGACCCTGTGGAAATCAACCTTGAAGGGATCAATCAAGTTAATGTAAGCACGA
AGGTGGGATTGGGTTTTGCCGAGGCACTTCGAGCCTTTTTGAGGCAAGACCCTGATGTCGTTATGGTTGGTGAGATTCGA
GACCTTGAAACGGCTTCGATCGCAATCAAGGCTGCGCAAACCGGCCACATGGTATTGTCGACCCTGCACACAAATAGTGC
TCCAGAAACATTGACTCGTATGCTTAATATGGGTGTTCCGGCTTTTAACATCGCCACATCCGTCAGTTTGATCATTGCCC
AGCGTCTCGCCCGGCGCTTGTGTAGCAACTGCAAAGAAAAAGCGGACATTCCGAAAGAGACGCTGTTAAAAGAAGGGTTC
CTGGAAGAACATTTCACCCCGGACTTTGCGCTCTATAAACCCAAAGGATGTGAAAAGTGTAATGACGGCTACAAAGGGCG
AGTCGGTATTTATGAAGTAGTGCGAGTAACACCATCAATATCACAACTCATTATGTCTGAAGCAAGTTCATTGGAAATCG
CGGCAGAGGCCCAGAAATGCGGATTCAATAATCTACGGCAATCGGCGATTCTCAAGGCCGTACAGGGGCTTACCAGTCTG
GAAGAAGTCAACCGAGTTACGAAGGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y0I693

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.32

98.77

0.596
  pilB Acinetobacter baylyi ADP1

57.447

99.121

0.569
  pilB Legionella pneumophila strain ERS1305867

55.556

99.649

0.554
  pilB Vibrio cholerae strain A1552

53.484

93.322

0.499
  pilB Vibrio parahaemolyticus RIMD 2210633

51.361

96.837

0.497
  pilB Vibrio campbellii strain DS40M4

49.911

98.946

0.494
  pilF Neisseria gonorrhoeae MS11

48.49

98.946

0.48
  pilB/pilB1 Synechocystis sp. PCC 6803

40.462

91.213

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.692

91.213

0.362

Multiple sequence alignment