Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CBR65_RS08195 Genome accession   NZ_CP021382
Coordinates   1984267..1985997 (+) Length   576 a.a.
NCBI ID   WP_087466409.1    Uniprot ID   A0A1Y0FVC5
Organism   Cellvibrio sp. PSBB006     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1974908..1987270 1984267..1985997 within 0


Gene organization within MGE regions


Location: 1974908..1987270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CBR65_RS08165 (CBR65_08150) - 1974908..1975456 (-) 549 WP_087466403.1 tetratricopeptide repeat protein -
  CBR65_RS08170 (CBR65_08155) - 1975472..1976314 (-) 843 WP_157672192.1 RHS repeat domain-containing protein -
  CBR65_RS08175 (CBR65_08160) - 1976460..1976687 (-) 228 WP_087466405.1 hypothetical protein -
  CBR65_RS08180 (CBR65_08165) - 1976700..1981223 (-) 4524 WP_198300909.1 RHS repeat-associated core domain-containing protein -
  CBR65_RS08185 (CBR65_08170) - 1981581..1983323 (-) 1743 WP_087466407.1 PglL family O-oligosaccharyltransferase -
  CBR65_RS08190 (CBR65_08175) - 1983374..1983883 (-) 510 WP_087466408.1 pilin -
  CBR65_RS08195 (CBR65_08180) pilB 1984267..1985997 (+) 1731 WP_087466409.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CBR65_RS08200 (CBR65_08185) pilC 1986002..1987270 (+) 1269 WP_087466410.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62897.97 Da        Isoelectric Point: 5.8555

>NTDB_id=230317 CBR65_RS08195 WP_087466409.1 1984267..1985997(+) (pilB) [Cellvibrio sp. PSBB006]
MNSPQPTLSGLARRLVTDGLLDQESARQAMSQAAKEKVPFVQHLVHHAILDARTIARAASDEFGTPLFDLDALNREAIPT
KLVDQKLIRKHHTLPLTRRGNRLFIAVADPTDLHALDEIKFNTGINTEAVLVEADKLATAIEKFLNAQEESIGDTLGGLD
EDGLDDLDIQAVDDQAASADQGASEADEAPIVRFINKVLLDAIKEGASDIHFEPYEKAYRVRFRTDGILHEVTRPPVNLA
TRMAARLKVMSQMDISERRVPQDGRIKMKISKTRAIDFRVNTLPTLFGEKVVLRILDPGAAKLGIDALGYEDNQKKLYLD
ALAQSQGMILVTGPTGSGKTVSLYTGLNILNTSETNISTAEDPVEINLEGINQVQMNTRVGLTFAEALRSFLRQDPDVIM
VGEIRDLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPAFNVATSVSLIIAQRLARRLCSNCKKPATDIPADI
LKQEGFDDIGIPPAEYQIFHPVGCNQCNKGYKGRLGVYEVVRITPVIASLIMEGGNSLQIAKAAREAGFNNLRVSALRKV
AMGLTSLEEANRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=230317 CBR65_RS08195 WP_087466409.1 1984267..1985997(+) (pilB) [Cellvibrio sp. PSBB006]
ATGAATAGCCCCCAACCTACACTGAGTGGCCTCGCCCGCCGCCTTGTGACAGACGGCCTGCTGGATCAAGAGAGCGCCCG
CCAAGCTATGAGCCAAGCCGCCAAGGAAAAAGTACCTTTTGTTCAACATCTGGTGCATCACGCCATACTTGATGCCCGAA
CCATTGCCCGGGCTGCCTCGGATGAATTCGGCACACCTTTATTTGATCTTGACGCGCTCAACCGTGAAGCAATTCCTACC
AAGCTGGTTGATCAAAAGCTAATCCGCAAGCACCACACACTACCGCTCACGCGCAGGGGCAACCGTCTTTTTATCGCAGT
AGCCGACCCTACTGACCTCCATGCGTTGGATGAGATCAAGTTTAATACCGGTATAAACACTGAAGCTGTATTAGTAGAAG
CAGATAAGTTGGCAACGGCTATCGAAAAATTTTTGAATGCCCAGGAAGAAAGCATTGGCGATACGCTCGGCGGGCTGGAT
GAAGATGGGTTAGATGACCTGGACATCCAGGCCGTAGATGACCAGGCTGCAAGCGCCGATCAAGGCGCATCAGAAGCCGA
TGAAGCACCTATCGTGCGTTTTATTAACAAAGTTCTGCTGGATGCCATTAAAGAAGGGGCTTCAGATATTCACTTTGAAC
CCTATGAGAAAGCCTATCGTGTCCGCTTTCGCACTGACGGCATCCTTCACGAAGTGACTCGCCCTCCGGTCAATTTAGCT
ACGCGTATGGCTGCCCGACTAAAAGTGATGTCGCAAATGGATATTTCAGAACGGCGGGTTCCTCAGGACGGCCGTATCAA
GATGAAAATATCCAAGACTCGCGCAATCGATTTTCGCGTAAACACCTTACCGACCTTGTTCGGAGAAAAGGTCGTATTAC
GGATTTTGGACCCCGGTGCAGCAAAGCTTGGGATTGATGCACTGGGTTATGAAGATAACCAGAAGAAACTCTATCTGGAC
GCCCTGGCTCAATCGCAAGGAATGATATTAGTCACCGGCCCTACCGGTAGTGGCAAGACAGTATCTCTTTATACCGGCTT
GAATATCTTAAACACGTCCGAAACCAACATTTCTACAGCAGAAGATCCCGTTGAGATCAATCTGGAAGGTATTAATCAAG
TCCAAATGAACACCAGGGTAGGACTAACCTTTGCCGAAGCGCTGCGCTCCTTCCTTCGCCAAGACCCGGATGTCATCATG
GTGGGTGAGATTCGGGACCTGGAAACTGCCGAAATTGCTATTAAAGCTGCACAAACAGGACACTTGGTTTTATCTACCCT
TCATACCAATAGCGCTCCAGAGACCTTGACGCGACTTTTAAATATGGGCGTTCCAGCGTTTAATGTCGCAACCAGCGTAA
GCTTGATCATTGCCCAAAGGCTTGCAAGGAGACTTTGTTCAAATTGTAAAAAGCCTGCTACAGATATTCCTGCAGATATA
CTTAAGCAAGAGGGCTTTGATGATATTGGCATACCACCTGCTGAGTACCAAATTTTTCACCCGGTTGGTTGTAACCAATG
CAACAAGGGTTATAAAGGTCGACTAGGTGTATATGAAGTGGTTCGCATCACCCCAGTCATTGCGAGCCTTATAATGGAAG
GCGGCAATTCCTTACAGATTGCCAAAGCCGCCAGGGAAGCCGGGTTTAACAATTTACGCGTCTCAGCCCTACGTAAAGTG
GCTATGGGACTCACCAGTCTCGAAGAAGCTAACCGAGTAACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-150)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y0FVC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.686

99.479

0.594
  pilB Acinetobacter baylyi ADP1

58.844

99.132

0.583
  pilB Legionella pneumophila strain ERS1305867

56.415

98.785

0.557
  pilB Vibrio campbellii strain DS40M4

49.653

100

0.497
  pilB Vibrio parahaemolyticus RIMD 2210633

49.739

99.826

0.497
  pilB Vibrio cholerae strain A1552

51.356

96.007

0.493
  pilF Neisseria gonorrhoeae MS11

48.161

99.132

0.477
  pilB/pilB1 Synechocystis sp. PCC 6803

37.255

100

0.396

Multiple sequence alignment