Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   GBS_RS01075 Genome accession   NC_004368
Coordinates   188052..189095 (+) Length   347 a.a.
NCBI ID   WP_223295592.1    Uniprot ID   -
Organism   Streptococcus agalactiae NEM316     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 183052..194095
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBS_RS01070 (gbs0158) - 186649..187014 (+) 366 WP_000285373.1 DUF1033 family protein -
  GBS_RS11090 comGA 187187..188158 (+) 972 WP_000250808.1 competence type IV pilus ATPase ComGA Machinery gene
  GBS_RS01075 (gbs0160) comGB 188052..189095 (+) 1044 WP_223295592.1 competence type IV pilus assembly protein ComGB Machinery gene
  GBS_RS01080 (gbs0161) comGC/cglC 189092..189421 (+) 330 WP_000793380.1 competence type IV pilus major pilin ComGC Machinery gene
  GBS_RS01085 (gbs0162) comGD 189396..189809 (+) 414 WP_000244259.1 competence type IV pilus minor pilin ComGD Machinery gene
  GBS_RS01090 (gbs0163) comGE 189781..190080 (+) 300 WP_001867089.1 competence type IV pilus minor pilin ComGE Machinery gene
  GBS_RS01095 (gbs0164) comGF 190034..190495 (+) 462 WP_001874060.1 competence type IV pilus minor pilin ComGF Machinery gene
  GBS_RS01100 (gbs0165) comGG 190473..190844 (+) 372 WP_000601104.1 competence type IV pilus minor pilin ComGG Machinery gene
  GBS_RS01105 (gbs0166) comGH 190959..191933 (+) 975 WP_001008570.1 class I SAM-dependent methyltransferase Machinery gene
  GBS_RS01110 (gbs0167) - 191965..193158 (+) 1194 WP_000047535.1 acetate kinase -
  GBS_RS01115 (gbs0168) - 193309..193515 (+) 207 WP_000798242.1 helix-turn-helix transcriptional regulator -
  GBS_RS11710 - 193574..193711 (+) 138 WP_001867090.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 40005.70 Da        Isoelectric Point: 9.9365

>NTDB_id=23019 GBS_RS01075 WP_223295592.1 188052..189095(+) (comGB) [Streptococcus agalactiae NEM316]
MDKWISWLKKDISVRNRHKSKKLSLKKQRKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDYYTDRMNKALLEGKDLSKML
GELGFSDTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLTFLVLIMIGLRHYLMPQLGENNFATS
LITNVPNIFLLLLAVVLIFSLIFYIVQKRLSRIKVACFLTTIPLVGSYVKLYLTAYYAREWGNLLSQGVELDQIVKVMQN
QKSKLFREIGYDMEEGFLSGKAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQPVI
FIFVALIIVMIYAAMLLPMYQNMEILS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=23019 GBS_RS01075 WP_223295592.1 188052..189095(+) (comGB) [Streptococcus agalactiae NEM316]
ATAGACAAGTGGATATCTTGGCTGAAGAAGGACATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAA
ACAACGGAAAGTAGTCCAACTTTTTAATAATCTTTTTGCTAGTGGTTTTTCTTTAACTGATATGGTCACCTTCTTAAAGA
GGAGTAAGTTATTGTCTGATTATTATACAGATAGGATGAATAAGGCATTATTAGAGGGAAAAGATTTATCAAAAATGTTA
GGAGAGTTAGGTTTTTCTGACACTGTTATCACACAGGTTGCATTAGCTGATTTGCATGGTAACATTTCAAGGAGCCTACT
AAAGATTGAGTCTTATTTAGCTAATCTTTTGTTAGTTAGAAAAAAAGTAATAGAAGTAGCTACTTACCCATTGATATTAT
TGACTTTTCTGGTGCTAATTATGATTGGTCTTAGGCATTATTTAATGCCCCAATTAGGAGAAAATAATTTTGCAACTAGC
CTGATTACAAATGTGCCGAATATTTTCTTATTACTTTTAGCAGTTGTACTTATTTTTAGTTTAATATTTTATATTGTTCA
AAAGCGATTGTCGCGTATTAAAGTAGCTTGTTTTTTAACAACAATTCCCTTAGTTGGATCATATGTTAAGCTTTATTTAA
CTGCTTACTATGCCCGTGAGTGGGGAAATTTATTAAGTCAAGGTGTTGAATTGGACCAAATTGTTAAAGTAATGCAAAAT
CAGAAATCCAAACTTTTTAGGGAAATAGGATATGACATGGAAGAAGGTTTTCTATCAGGTAAAGCATTTCACCAAAAAGT
ATTAGACTATCCGTTTTTCTTAACTGAGCTTAGTTTAATGATTGAATATGGCCAAGTTAAGGCGAAATTAGGAACAGAGT
TAGATATATATGCTGATGAGAAGTGGGAGGATTTTTTTACAAAATTAGCTAGAGCGACCCAGTTAATCCAACCTGTTATT
TTTATTTTTGTAGCTCTTATCATTGTTATGATTTATGCAGCAATGCTGTTACCAATGTATCAAAATATGGAGATATTATC
ATGA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(218-338)

Type II secretion system (T2SS), protein F (T2SSF)

(33-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Streptococcus mutans UA140

61.877

98.271

0.608
  comGB Streptococcus mutans UA159

61.584

98.271

0.605
  comGB Streptococcus gordonii str. Challis substr. CH1

56.305

98.271

0.553
  comGB/cglB Streptococcus mitis NCTC 12261

54.925

96.542

0.53
  comGB/cglB Streptococcus mitis SK321

54.328

96.542

0.524
  comGB/cglB Streptococcus pneumoniae Rx1

53.433

96.542

0.516
  comGB/cglB Streptococcus pneumoniae D39

53.433

96.542

0.516
  comGB/cglB Streptococcus pneumoniae R6

53.433

96.542

0.516
  comGB/cglB Streptococcus pneumoniae TIGR4

53.433

96.542

0.516
  comGB Lactococcus lactis subsp. cremoris KW2

49.852

97.118

0.484

Multiple sequence alignment