Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MTMN5_RS14370 Genome accession   NZ_CP021333
Coordinates   3125483..3127198 (-) Length   571 a.a.
NCBI ID   WP_126812065.1    Uniprot ID   -
Organism   Marinobacter salarius strain HL2708#2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3120483..3132198
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTMN5_RS14350 (MTMN5_02902) tsaA 3121944..3122690 (-) 747 WP_126812057.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  MTMN5_RS14355 (MTMN5_02903) coaE 3122696..3123298 (-) 603 WP_126812059.1 dephospho-CoA kinase -
  MTMN5_RS14360 (MTMN5_02904) pilD 3123299..3124174 (-) 876 WP_126812061.1 A24 family peptidase Machinery gene
  MTMN5_RS14365 (MTMN5_02905) pilC 3124260..3125480 (-) 1221 WP_126812063.1 type II secretion system F family protein Machinery gene
  MTMN5_RS14370 (MTMN5_02906) pilB 3125483..3127198 (-) 1716 WP_126812065.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MTMN5_RS14375 (MTMN5_02907) - 3127263..3127505 (+) 243 WP_126812067.1 hypothetical protein -
  MTMN5_RS14380 (MTMN5_02908) - 3127597..3128082 (+) 486 WP_164732152.1 pilin -
  MTMN5_RS14385 (MTMN5_02909) - 3128205..3129722 (+) 1518 WP_126812071.1 O-antigen ligase -
  MTMN5_RS14390 (MTMN5_02910) - 3129782..3130225 (+) 444 WP_126812073.1 pilin -
  MTMN5_RS21390 - 3130282..3131171 (-) 890 Protein_2851 MBL fold metallo-hydrolase RNA specificity domain-containing protein -
  MTMN5_RS14400 (MTMN5_02912) cysQ 3131304..3132071 (+) 768 WP_126812075.1 3'(2'),5'-bisphosphate nucleotidase CysQ -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62808.77 Da        Isoelectric Point: 4.9580

>NTDB_id=229978 MTMN5_RS14370 WP_126812065.1 3125483..3127198(-) (pilB) [Marinobacter salarius strain HL2708#2]
MSTNKSNVTLTGLARRFVDDGLIDESTAKDAFLQASHNRIPLITYLVQNNLANSGGLAFSAAMEFGVSVLDLNAFMPEMM
PDKVVDEKLIRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAILVDDAKLREAIDKYLESHDTSMGDLDDA
DLEGVETEGGDQEDDAVAATSDVDDAPIVKYVNKMLLDAIRGGASDVHFEPYEKTYRVRYRTDGILKEMSRPSIKLAPKI
SARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEDEQKQLYMEALE
QPQGMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPAEINLEGINQVNVNTRVGLGFAEALRAFLRQDPDIIMVGE
IRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPSFNIATSVSLIIAQRLGRRLCNSCRQPADIPKDVLLAE
GFTQEQIDTGFTLYRPKGCDKCNGGYKGRVGIYEVVKITEELANMIMEEASSIKIAKQAQAEGFPNLRQSALKKVIEGVT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=229978 MTMN5_RS14370 WP_126812065.1 3125483..3127198(-) (pilB) [Marinobacter salarius strain HL2708#2]
ATGTCTACCAATAAAAGCAATGTAACGCTTACTGGCCTGGCGAGACGCTTCGTCGATGACGGGCTTATTGATGAATCCAC
CGCGAAAGATGCCTTTCTTCAGGCGTCCCACAACCGCATTCCACTCATTACCTACCTGGTACAGAACAACCTGGCGAACA
GCGGAGGGCTCGCATTCTCTGCTGCGATGGAGTTCGGCGTTTCGGTTCTGGATCTCAACGCCTTTATGCCGGAGATGATG
CCCGATAAGGTCGTAGACGAAAAACTGATACGAAAACACAACGCCCTGCCGCTCTATAAGCGGGGGAACCGGCTGTTTAT
TGCGGTCTCTGACCCGACCAACATCCAGGCGCTGGACGAAATCAAGTTTAATACCGGCCTGAGCACTGACGCCATCCTAG
TTGACGACGCCAAGCTCCGGGAAGCCATTGACAAGTATCTTGAGTCACACGATACGTCGATGGGTGATCTGGATGACGCC
GACCTTGAAGGCGTTGAAACCGAAGGCGGTGACCAGGAAGACGATGCCGTTGCCGCAACGAGCGATGTCGACGATGCCCC
CATCGTAAAGTACGTGAACAAGATGCTTCTTGACGCCATTCGCGGGGGTGCCTCGGACGTTCACTTTGAACCCTACGAGA
AAACCTATCGGGTTCGGTACCGCACCGATGGCATTCTGAAAGAAATGTCGCGCCCCTCGATCAAGCTGGCGCCCAAGATT
TCCGCCCGGGTAAAGATCATGGCGCAGCTGGATATCTCGGAGCGCCGGGTCCCCCAGGACGGTCGAATCAAGATGAAGCT
CTCCAAGACCAAGGCCATCGACTTCCGGGTCAATACCCTGCCGACACTCTGGGGAGAAAAGATCGTTCTGCGGATTCTGG
ACCCCAGCCAGGCCAAGCTGGGCATCGACGTTCTGGGCTATGAAGATGAGCAGAAGCAGCTATACATGGAGGCGCTGGAG
CAGCCGCAAGGCATGATTCTGGTAACCGGCCCGACAGGCTCGGGCAAAACGGTCTCTCTCTATACCGGTCTCAACATACT
GAACACGCCTGAGCGGAACATTTCCACAGCCGAAGACCCCGCGGAAATCAACCTGGAAGGCATTAACCAGGTCAACGTCA
ACACCAGGGTGGGCCTCGGCTTTGCGGAAGCACTGAGAGCCTTTCTGCGTCAGGATCCGGACATCATCATGGTGGGTGAG
ATCCGAGACCTGGAAACGGCGAACATCGCTATCAAAGCAGCGCAAACCGGGCACCTGGTGCTCTCGACCCTGCACACCAA
CAGCGCTGCGGAAACGCTCACGCGCATGATGAACATGGGCGTTCCGTCCTTCAACATCGCCACGTCGGTGAGCCTGATCA
TTGCCCAGCGACTGGGCCGTCGGCTTTGTAACTCTTGTAGACAACCTGCCGACATCCCCAAAGACGTGCTTTTAGCGGAG
GGGTTCACACAGGAACAAATTGATACAGGGTTCACGTTGTACCGTCCCAAGGGCTGTGATAAATGCAATGGTGGATACAA
AGGCCGCGTCGGGATTTACGAGGTGGTCAAGATTACCGAGGAGCTGGCGAACATGATTATGGAAGAAGCCAGTTCGATTA
AAATTGCAAAGCAGGCACAGGCCGAAGGCTTCCCGAACTTGCGGCAGTCTGCCCTCAAAAAAGTCATTGAGGGCGTGACC
AGTCTAGAGGAAGCCAACCGCGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(21-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.068

100

0.573
  pilB Acinetobacter baylyi ADP1

56.993

100

0.571
  pilB Legionella pneumophila strain ERS1305867

54.947

99.124

0.545
  pilB Vibrio parahaemolyticus RIMD 2210633

51.706

97.548

0.504
  pilB Vibrio campbellii strain DS40M4

50.709

98.774

0.501
  pilB Vibrio cholerae strain A1552

53.654

91.068

0.489
  pilF Neisseria gonorrhoeae MS11

47.527

99.124

0.471
  pilB/pilB1 Synechocystis sp. PCC 6803

41.313

90.718

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.114

92.119

0.37

Multiple sequence alignment