Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   MTMN5_RS04250 Genome accession   NZ_CP021333
Coordinates   926663..928744 (-) Length   693 a.a.
NCBI ID   WP_177190642.1    Uniprot ID   -
Organism   Marinobacter salarius strain HL2708#2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 921663..933744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTMN5_RS04235 (MTMN5_00853) - 923382..924851 (-) 1470 WP_227544274.1 AAA family ATPase -
  MTMN5_RS04240 (MTMN5_00854) aroB 924881..925975 (-) 1095 WP_075195818.1 3-dehydroquinate synthase -
  MTMN5_RS04245 (MTMN5_00855) aroK 925968..926534 (-) 567 WP_036203590.1 shikimate kinase AroK -
  MTMN5_RS04250 (MTMN5_00856) pilQ 926663..928744 (-) 2082 WP_177190642.1 type IV pilus secretin PilQ Machinery gene
  MTMN5_RS04255 (MTMN5_00857) - 928811..929359 (-) 549 WP_126810589.1 pilus assembly protein PilP -
  MTMN5_RS04260 (MTMN5_00858) - 929362..929973 (-) 612 WP_041335800.1 type 4a pilus biogenesis protein PilO -
  MTMN5_RS04265 (MTMN5_00859) - 929973..930536 (-) 564 WP_041335802.1 PilN domain-containing protein -
  MTMN5_RS04270 (MTMN5_00860) pilM 930536..931600 (-) 1065 WP_036203576.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 74224.92 Da        Isoelectric Point: 4.2835

>NTDB_id=229964 MTMN5_RS04250 WP_177190642.1 926663..928744(-) (pilQ) [Marinobacter salarius strain HL2708#2]
MFRKLNVYVGVIAFGLLSGLANAVTLEDVSFSSLSGDRVEVTLAFDGQPPEPTGYTIERPARIAVDLRDTTSGLDSRNIS
LGGGNAQSMTVVETKDRTRLIFNLAELAPYETVRTQNSLVVTIGSQGSGVAASGSTSSSTPMTTASGGQSVSSAGNDLAG
VDFRRGQNGEGRVIVDLGSANASVDLSELGGKIRLTMDGTSVPDNLRRRLDVTDFATPVTRVDTYTEDGNAVVEIRPEGN
YDYIAYQSGSQFTVSVERLTEEEAESRREEKFPYTGDKLSLNFQDIEVRSVLQLIADFTGLNLVASDTVSGSITLRLQNV
PWDQALDLILKTKGLDKRQIGNVLLVAPADEIAAREKLELETSKQIAELAPVRLDIIQVNYAKAADVVELIRADEELISS
RGFVSSDARTNTISVRETSEKLDEIRRLVSTWDVPVRQVSIEARIVRAQTNVAENLGVRWGGAAYDVSGDNVFSVGGSQN
AVEEARQAAGGTNNTITFPDALAVDLGVTGQGASSFAIGLGSDDFLVDLELSALESDGQAEVVSQPRVVTADRQTAKIKS
GEEIPYQEASSSGATSVSFKEAALALEVTPQITPDDKIIMDLVVNQDSRGEVTAGIPAINTNEVTTQVLVGNGETVVLGG
IFQSEVTTTTTKTPFLGDIPYLGRLFKRTEHVDERSELLIFITPKIIKSDLIQ

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=229964 MTMN5_RS04250 WP_177190642.1 926663..928744(-) (pilQ) [Marinobacter salarius strain HL2708#2]
ATGTTCAGAAAACTCAATGTATACGTCGGCGTGATTGCTTTTGGGTTGTTATCCGGCCTGGCCAATGCGGTCACGCTGGA
AGACGTGTCGTTTTCGTCGCTGTCCGGGGATCGGGTCGAGGTGACGCTGGCGTTTGACGGCCAGCCACCAGAACCGACCG
GGTACACGATCGAGCGACCTGCCAGAATCGCAGTGGACCTCCGGGACACGACCAGCGGTCTCGACAGTCGCAACATCTCG
CTCGGGGGAGGTAACGCCCAGAGCATGACGGTTGTCGAAACCAAGGATCGCACTCGCCTGATCTTCAACCTGGCAGAGCT
GGCTCCCTATGAGACGGTGCGTACCCAGAACAGCCTGGTCGTCACAATCGGCAGTCAGGGTAGTGGTGTCGCAGCGTCAG
GCTCGACTTCTTCCTCTACACCGATGACTACTGCATCGGGAGGACAGTCGGTCTCATCGGCAGGCAACGACCTGGCGGGA
GTCGATTTTAGGCGTGGGCAGAATGGCGAAGGACGCGTGATTGTAGATCTGGGTAGTGCCAATGCATCCGTGGATCTGTC
CGAGCTCGGGGGCAAGATTCGCTTGACGATGGACGGTACCAGTGTGCCGGACAATCTTCGTCGCCGCCTTGATGTTACCG
ATTTCGCGACACCGGTAACCCGGGTTGATACCTACACGGAAGATGGTAATGCGGTTGTGGAAATTCGTCCGGAAGGCAAC
TACGACTACATTGCCTACCAGTCCGGTTCGCAGTTTACCGTCAGCGTGGAAAGGCTGACAGAAGAGGAAGCTGAGTCTCG
TCGAGAGGAGAAGTTCCCGTACACCGGCGACAAGCTCTCTCTCAACTTCCAGGACATCGAAGTTCGTTCGGTATTGCAGT
TGATTGCCGACTTTACTGGTCTGAACCTTGTTGCCAGCGATACGGTCAGTGGCAGCATCACGCTGCGTTTGCAGAATGTG
CCCTGGGACCAGGCTCTGGACCTGATCCTTAAGACCAAGGGATTGGACAAGCGTCAGATTGGTAACGTACTGCTGGTTGC
TCCGGCCGATGAAATCGCCGCCCGTGAAAAGCTTGAGCTGGAGACGTCCAAGCAGATCGCTGAGCTCGCACCGGTTCGCC
TGGATATCATTCAGGTGAATTACGCCAAGGCTGCCGATGTCGTTGAACTGATTAGGGCGGATGAAGAACTTATCTCCTCT
CGGGGATTCGTCTCTTCAGATGCGCGTACCAACACGATCAGTGTTCGTGAAACTTCGGAAAAGCTTGATGAAATTCGCCG
CCTGGTTTCGACCTGGGATGTTCCTGTTCGTCAGGTTTCCATTGAGGCTCGAATTGTAAGAGCGCAAACCAACGTTGCCG
AAAATCTCGGTGTTCGTTGGGGTGGTGCCGCCTACGATGTCAGTGGTGATAACGTTTTCTCAGTGGGTGGTTCGCAGAAC
GCGGTTGAGGAGGCGCGTCAAGCCGCTGGTGGTACCAACAACACGATCACCTTCCCGGACGCTCTGGCGGTAGATTTGGG
TGTCACCGGCCAAGGTGCTTCCTCATTTGCCATCGGCTTGGGCAGTGATGATTTTCTTGTGGACCTGGAACTCTCTGCAC
TGGAGAGTGACGGACAGGCGGAAGTTGTTTCCCAGCCCCGCGTTGTTACTGCCGATCGCCAGACCGCCAAGATTAAGTCC
GGTGAGGAGATCCCTTATCAAGAGGCTTCTTCCAGCGGTGCGACGTCAGTATCTTTCAAGGAAGCGGCGTTGGCTCTTGA
AGTAACCCCTCAGATCACCCCGGATGACAAAATCATCATGGATCTGGTGGTTAATCAGGACTCCCGTGGTGAAGTGACCG
CCGGTATTCCTGCCATCAACACTAATGAGGTAACCACTCAGGTGTTGGTTGGAAATGGCGAAACCGTCGTTCTGGGCGGT
ATTTTCCAGTCAGAGGTTACGACGACGACTACCAAGACTCCGTTCCTGGGTGATATTCCCTACCTCGGCCGCCTCTTCAA
GCGCACTGAGCATGTTGATGAGCGTAGCGAACTGTTGATCTTCATCACACCGAAGATCATCAAGAGCGACCTGATTCAGT
AA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(36-123)

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II and III secretion system protein (Secretin)

(533-688)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-438)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

51.159

99.567

0.509
  pilQ Acinetobacter baumannii strain A118

40.816

100

0.433
  pilQ Acinetobacter baumannii D1279779

40.816

100

0.433
  comQ Acinetobacter baylyi ADP1

41.011

100

0.421
  pilQ Legionella pneumophila strain ERS1305867

40.768

97.691

0.398
  pilQ Legionella pneumophila strain Lp02

40.768

97.691

0.398

Multiple sequence alignment