Detailed information NTG0229965

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: MTMN5_RS04270
• Genome accession: NZ_CP021333
• Coordinates: 930536..931600 (-)
• Length: 354 a.a.
• NCBI ID: WP_036203576.1
• Uniprot ID: A0A1W6KDI5
• Organism: Marinobacter salarius strain HL2708#2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 925536..936600

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MTMN5_RS04245 (MTMN5_00855)	aroK	925968..926534 (-)	567	WP_036203590.1	shikimate kinase AroK	-
MTMN5_RS04250 (MTMN5_00856)	pilQ	926663..928744 (-)	2082	WP_177190642.1	type IV pilus secretin PilQ	Machinery gene
MTMN5_RS04255 (MTMN5_00857)	-	928811..929359 (-)	549	WP_126810589.1	pilus assembly protein PilP	-
MTMN5_RS04260 (MTMN5_00858)	-	929362..929973 (-)	612	WP_041335800.1	type 4a pilus biogenesis protein PilO	-
MTMN5_RS04265 (MTMN5_00859)	-	929973..930536 (-)	564	WP_041335802.1	PilN domain-containing protein	-
MTMN5_RS04270 (MTMN5_00860)	pilM	930536..931600 (-)	1065	WP_036203576.1	pilus assembly protein PilM	Machinery gene
MTMN5_RS04275 (MTMN5_00861)	-	931815..934301 (+)	2487	WP_036203572.1	penicillin-binding protein 1A	-
MTMN5_RS04280 (MTMN5_00862)	-	934392..935657 (-)	1266	WP_036203569.1	malic enzyme-like NAD(P)-binding protein	-
MTMN5_RS04285 (MTMN5_00863)	rpmE	935890..936111 (-)	222	WP_007154619.1	50S ribosomal protein L31	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38048.10 Da
• Isoelectric Point: 4.1243

>NTDB_id=229965 MTMN5_RS04270 WP_036203576.1 930536..931600(-) (pilM) [Marinobacter salarius strain HL2708#2]
MFGLLGKKSSSVLGVDISSSSVKLLELSKQGDRYKVESYAVEPLPANAVVEKNITDVEAVGEVLKRVASKSRTSVKQVAV
AVSGSAVITKLIQMDGGLNEFEMEDQITLEADQYIPYPLDEVAIDFEVQGPSEANPDQVDVLLAACRKENVDIREDALEI
ASLTTKIVDVEAYALERAYALIEPQLDSQGEELVVAIVDIGATMTTLSVLAEGKTVYTREQIFGGKQLTEEIQRRYGLSL
EEAGLAKKQGGLPDDYDSEVLTPFREAVVQQVARALQFFFGASQYNAVDYVVLAGGTASIQGLTEMVEEKTGTPTLVANP
FADMAVGSRVNASSLSNDAPSLMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=229965 MTMN5_RS04270 WP_036203576.1 930536..931600(-) (pilM) [Marinobacter salarius strain HL2708#2]
GTGTTCGGATTGTTAGGAAAAAAATCCAGTTCGGTGCTGGGCGTGGATATCAGTTCCAGCTCGGTCAAGTTACTGGAACT
ATCGAAGCAGGGCGACCGTTACAAGGTCGAGAGCTACGCAGTGGAGCCGCTACCGGCCAATGCTGTGGTAGAGAAAAACA
TCACGGATGTCGAGGCGGTGGGCGAAGTGCTCAAACGTGTCGCGTCCAAATCACGTACGAGCGTCAAGCAGGTGGCTGTG
GCCGTCTCCGGTTCCGCCGTTATTACCAAGCTGATCCAGATGGACGGTGGTCTTAACGAGTTTGAGATGGAAGACCAGAT
CACGCTCGAGGCCGATCAGTATATTCCCTATCCGTTGGATGAAGTGGCAATCGACTTCGAGGTTCAGGGGCCCTCTGAGG
CTAACCCGGATCAGGTCGATGTACTTCTGGCTGCGTGTCGCAAAGAAAACGTCGATATTCGGGAAGACGCTCTGGAGATT
GCTTCCTTGACCACCAAGATTGTGGACGTCGAAGCATACGCACTCGAGCGGGCATACGCCCTCATCGAGCCTCAGCTTGA
TTCCCAGGGTGAAGAACTCGTTGTGGCGATCGTCGATATCGGCGCAACCATGACCACACTGAGCGTGTTGGCTGAAGGCA
AGACGGTTTACACTCGCGAGCAGATTTTCGGGGGGAAACAACTCACCGAGGAAATTCAGCGTCGTTATGGTCTGTCCCTT
GAAGAGGCCGGCCTTGCCAAGAAGCAGGGTGGCCTCCCCGATGACTACGATTCCGAAGTGCTGACGCCTTTCCGGGAAGC
GGTAGTTCAGCAGGTTGCGAGGGCGCTGCAGTTCTTCTTCGGCGCAAGCCAGTACAATGCGGTTGACTACGTAGTTCTGG
CTGGCGGCACGGCATCTATCCAGGGGCTGACAGAAATGGTCGAGGAAAAAACGGGCACACCCACGCTGGTTGCAAATCCG
TTCGCGGACATGGCAGTGGGATCAAGGGTGAATGCGTCTTCGCTGAGCAATGATGCGCCTTCACTCATGATTGCCTGTGG
GCTGGCAATGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A1W6KDI5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	53.955	100	0.54
comM	Acinetobacter nosocomialis M2	53.672	100	0.537
comM	Acinetobacter baylyi ADP1	51.695	100	0.517
pilM	Legionella pneumophila strain ERS1305867	45.763	100	0.458