Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CA924_RS05120 Genome accession   NZ_CP021281
Coordinates   1084551..1085615 (+) Length   354 a.a.
NCBI ID   WP_011213353.1    Uniprot ID   A0A3A6VL78
Organism   Legionella pneumophila subsp. pneumophila strain Flint     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1079551..1090615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CA924_RS05110 - 1080593..1082977 (-) 2385 WP_011213351.1 penicillin-binding protein 1A -
  CA924_RS05115 - 1083127..1084134 (-) 1008 WP_011213352.1 hypothetical protein -
  CA924_RS05120 pilM 1084551..1085615 (+) 1065 WP_011213353.1 type IV pilus assembly protein PilM Machinery gene
  CA924_RS05125 pilN 1085619..1086167 (+) 549 WP_011213354.1 PilN domain-containing protein Machinery gene
  CA924_RS05130 pilO 1086181..1086780 (+) 600 WP_011213355.1 type 4a pilus biogenesis protein PilO Machinery gene
  CA924_RS05135 pilP 1086777..1087361 (+) 585 WP_011213356.1 pilus assembly protein PilP Machinery gene
  CA924_RS05140 pilQ 1087365..1089461 (+) 2097 WP_011213357.1 type IV pilus secretin PilQ Machinery gene
  CA924_RS05145 aroK 1089907..1090434 (+) 528 WP_010946667.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38788.79 Da        Isoelectric Point: 5.0367

>NTDB_id=229621 CA924_RS05120 WP_011213353.1 1084551..1085615(+) (pilM) [Legionella pneumophila subsp. pneumophila strain Flint]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKRLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=229621 CA924_RS05120 WP_011213353.1 1084551..1085615(+) (pilM) [Legionella pneumophila subsp. pneumophila strain Flint]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAGATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTCGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAATCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGACAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCACCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3A6VL78

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

99.718

100

0.997
  comM Acinetobacter nosocomialis M2

42.655

100

0.427
  pilM Acinetobacter baumannii D1279779

42.373

100

0.424
  comM Acinetobacter baylyi ADP1

40.96

100

0.41

Multiple sequence alignment