Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   CA924_RS05125 Genome accession   NZ_CP021281
Coordinates   1085619..1086167 (+) Length   182 a.a.
NCBI ID   WP_011213354.1    Uniprot ID   A0A128KQU2
Organism   Legionella pneumophila subsp. pneumophila strain Flint     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1080619..1091167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CA924_RS05115 - 1083127..1084134 (-) 1008 WP_011213352.1 hypothetical protein -
  CA924_RS05120 pilM 1084551..1085615 (+) 1065 WP_011213353.1 type IV pilus assembly protein PilM Machinery gene
  CA924_RS05125 pilN 1085619..1086167 (+) 549 WP_011213354.1 PilN domain-containing protein Machinery gene
  CA924_RS05130 pilO 1086181..1086780 (+) 600 WP_011213355.1 type 4a pilus biogenesis protein PilO Machinery gene
  CA924_RS05135 pilP 1086777..1087361 (+) 585 WP_011213356.1 pilus assembly protein PilP Machinery gene
  CA924_RS05140 pilQ 1087365..1089461 (+) 2097 WP_011213357.1 type IV pilus secretin PilQ Machinery gene
  CA924_RS05145 aroK 1089907..1090434 (+) 528 WP_010946667.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 21236.95 Da        Isoelectric Point: 10.1404

>NTDB_id=229622 CA924_RS05125 WP_011213354.1 1085619..1086167(+) (pilN) [Legionella pneumophila subsp. pneumophila strain Flint]
MTEINLLPWREQKREQEKKLFTTMLLIGIIIAAAIVFLMNSYVSHLISNQTNRNQILQKEITALDEQIKEIKALKQIREG
LISRMSIVQNLQSTRTLMVHLFDELIKITPPGVYITKMQREHDVVTLWGYSESNTSISILMRNIEANVWIQNPVLTEIKK
NVEKKQSPNNEFKLSFVLKPKY

Nucleotide


Download         Length: 549 bp        

>NTDB_id=229622 CA924_RS05125 WP_011213354.1 1085619..1086167(+) (pilN) [Legionella pneumophila subsp. pneumophila strain Flint]
ATGACTGAAATTAATTTATTGCCATGGCGTGAACAAAAACGTGAGCAGGAAAAGAAATTATTTACAACCATGCTATTGAT
AGGGATTATCATCGCTGCGGCAATTGTTTTTTTAATGAACTCTTATGTCTCTCATTTAATAAGTAATCAAACGAACCGAA
ATCAGATTTTACAAAAAGAAATTACAGCTTTGGATGAACAGATCAAGGAGATTAAAGCATTAAAACAAATCAGGGAAGGT
TTGATTTCAAGAATGTCGATTGTGCAGAATCTGCAGTCAACAAGAACTTTAATGGTTCACTTATTTGATGAGTTAATAAA
AATTACTCCTCCAGGAGTTTACATAACCAAGATGCAAAGAGAACACGATGTGGTAACACTTTGGGGGTATTCCGAATCTA
ATACAAGTATTTCTATTTTGATGAGAAATATAGAGGCCAATGTCTGGATACAGAATCCTGTTCTAACTGAAATTAAAAAA
AATGTTGAAAAAAAACAGTCTCCAAACAATGAATTTAAATTAAGTTTTGTGCTTAAACCCAAATATTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A128KQU2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

100

100

1

Multiple sequence alignment