Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CAY59_RS03765 Genome accession   NZ_CP021145
Coordinates   745297..746982 (-) Length   561 a.a.
NCBI ID   WP_086368982.1    Uniprot ID   -
Organism   Vibrio campbellii strain LA16-V1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 740297..751982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CAY59_RS03735 (CAY59_03690) rplS 740659..741012 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  CAY59_RS03740 (CAY59_03700) yacG 741459..741659 (-) 201 WP_012128882.1 DNA gyrase inhibitor YacG -
  CAY59_RS03745 (CAY59_03705) zapD 741716..742456 (-) 741 WP_012128881.1 cell division protein ZapD -
  CAY59_RS03750 (CAY59_03710) coaE 742484..743098 (-) 615 WP_012128880.1 dephospho-CoA kinase -
  CAY59_RS03755 (CAY59_03715) pilD 743099..743968 (-) 870 WP_045395355.1 A24 family peptidase Machinery gene
  CAY59_RS03760 (CAY59_03720) - 744041..745262 (-) 1222 Protein_621 type II secretion system F family protein -
  CAY59_RS03765 (CAY59_03725) pilB 745297..746982 (-) 1686 WP_086368982.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CAY59_RS03770 (CAY59_03730) - 746982..747416 (-) 435 WP_050917749.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CAY59_RS03780 (CAY59_03740) nadC 747682..748569 (-) 888 WP_050917751.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CAY59_RS03785 (CAY59_03745) ampD 748662..749213 (+) 552 WP_038891990.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CAY59_RS03790 (CAY59_03750) pdhR 749618..750385 (+) 768 WP_005428674.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62051.81 Da        Isoelectric Point: 5.1851

>NTDB_id=228566 CAY59_RS03765 WP_086368982.1 745297..746982(-) (pilB) [Vibrio campbellii strain LA16-V1]
MLTNLSTVLRQAGILTFSQEESLLEQAKASGISMPEALLSSGFFQSHELTEHLSSVFGLSCTSLNQYEYASLCQTLGLRD
LITRHNALPLNRTSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRSLGQEKSGLKEINQDELAG
LVDIGEDEIENIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYENMYRVRLRCDGILIEIQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSAALNINKLGYSDSQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLPGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCPYCRQPQEPNSQLQHIGITSTEQ
IFQANPDGCNECTHGYSGRTGIYEVMKFDESLSEALIKGASVHELEKLAIANGMQTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=228566 CAY59_RS03765 WP_086368982.1 745297..746982(-) (pilB) [Vibrio campbellii strain LA16-V1]
ATGCTAACCAACCTCTCAACAGTTCTTCGCCAAGCAGGAATACTGACCTTTTCCCAAGAAGAATCGTTGCTTGAGCAAGC
AAAAGCTTCTGGCATTTCGATGCCGGAGGCTTTACTCAGTTCTGGCTTTTTCCAATCTCATGAATTGACAGAACACCTAA
GCTCTGTATTCGGGCTTTCTTGCACTTCTCTTAACCAATACGAATACGCTTCGCTATGCCAAACGCTTGGCTTACGCGAT
CTCATCACTCGCCATAACGCTTTGCCGCTTAACCGCACCTCTTCAACTTTAATTCTCGCGGTAGCTGACCCAACCAACCT
TCAAGCCGAAGATGACTTTCGTTTTGCAACCGGTTTGCAAGTTGAGCTTGTCCTCGCTGACTTTCGAGAGCTGACAGCAG
CCATTCGGCGTTTATATGGCCGATCACTTGGGCAAGAAAAATCCGGTCTTAAAGAAATCAACCAAGATGAACTGGCGGGT
TTGGTTGATATTGGTGAGGATGAAATTGAGAACATCGAAGATCTCAGCCAAGATGAATCGCCGGTCAGTCGCTATATCAA
CCAAATTTTACTCGATGCTGTCCGTAAAGGGGCTTCCGATATTCACTTCGAGCCCTATGAAAACATGTACCGCGTGCGCT
TGCGTTGCGATGGCATCTTAATTGAAATTCAGCAGCCTCCGAGTCATTTAAGCCGTCGCCTTTCAGCTCGGATAAAAATC
TTATCTAAGCTTGATATCGCCGAGCGCCGTTTACCACAGGATGGGCGAATCAAGCTCAAATTAAACCAAGACACTGCAAT
TGATATGCGTGTCTCCACACTACCAACTCTTTTTGGGGAAAAGATTGTTCTGCGTTTGCTCGACAGCAGTTCTGCAGCAT
TAAATATCAATAAGCTCGGTTATAGCGACTCACAGAAACAACTTTATTTGGATGCCCTGCGCCGTCCGCAAGGTATGATC
CTGATGACAGGCCCAACAGGTAGCGGTAAAACGGTCTCGCTCTATACTGGATTGAGCATTCTTAATAAACCAGAGATCAA
TATCTCAACCGCTGAAGATCCCGTAGAGATTAACCTGCCCGGCATTAACCAAGTTCAAGTTCAGCCAAAGATCGGCTTTG
GTTTTGCAGAGGCGCTTCGCTCTTTCTTACGTCAAGACCCTGATGTAGTCATGGTGGGCGAGATCCGAGATCTTGACACA
GCAGAAATCGCGATCAAAGCATCACAGACTGGCCACTTAGTTCTCTCCACTTTACACACAAACTCAGCTGCCGAGACTGT
TATTCGCTTGTCTAATATGGGCGTAGAAAGCTTTAACCTCGCCTCTTCTCTCAGTTTGATCATCGCCCAGCGTTTGGCTA
GAAAACTTTGTCCATATTGTAGACAACCTCAAGAGCCAAACAGCCAACTTCAACATATTGGAATCACCAGCACCGAACAG
ATCTTTCAAGCTAATCCCGACGGTTGTAACGAGTGTACCCATGGTTATTCAGGGCGAACCGGCATTTACGAAGTGATGAA
ATTTGATGAATCCCTTTCAGAAGCACTCATCAAAGGCGCTTCAGTACACGAATTAGAAAAACTCGCCATTGCCAATGGTA
TGCAAACCCTACAAATGTCTGGTATCGAAAAACTCAAACAAGGCATTACCAGCTTCAGCGAATTGCAGCGCGTTCTCTAC
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

98.93

100

0.989
  pilB Vibrio parahaemolyticus RIMD 2210633

90.374

100

0.904
  pilB Vibrio cholerae strain A1552

73.31

100

0.734
  pilB Acinetobacter baylyi ADP1

47.935

99.287

0.476
  pilB Acinetobacter baumannii D1279779

51.252

92.513

0.474
  pilB Legionella pneumophila strain ERS1305867

48.524

96.613

0.469
  pilF Neisseria gonorrhoeae MS11

43.794

100

0.44
  pilB/pilB1 Synechocystis sp. PCC 6803

37.037

100

0.392
  pilF Thermus thermophilus HB27

36.833

100

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.559

99.465

0.364

Multiple sequence alignment