Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MARSALSMR5_RS06985 Genome accession   NZ_CP020931
Coordinates   1564257..1565972 (-) Length   571 a.a.
NCBI ID   WP_085679891.1    Uniprot ID   -
Organism   Marinobacter salarius strain SMR5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1559257..1570972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MARSALSMR5_RS06965 (MARSALSMR5_01425) tsaA 1560718..1561464 (-) 747 WP_036209925.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  MARSALSMR5_RS06970 (MARSALSMR5_01426) coaE 1561470..1562072 (-) 603 WP_085679884.1 dephospho-CoA kinase -
  MARSALSMR5_RS06975 (MARSALSMR5_01427) pilD 1562073..1562948 (-) 876 WP_085679887.1 A24 family peptidase Machinery gene
  MARSALSMR5_RS06980 (MARSALSMR5_01428) pilC 1563034..1564254 (-) 1221 WP_085679889.1 type II secretion system F family protein Machinery gene
  MARSALSMR5_RS06985 (MARSALSMR5_01429) pilB 1564257..1565972 (-) 1716 WP_085679891.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MARSALSMR5_RS06990 (MARSALSMR5_01430) - 1566037..1566279 (+) 243 WP_085679894.1 hypothetical protein -
  MARSALSMR5_RS06995 (MARSALSMR5_01431) - 1566356..1566859 (+) 504 WP_319830337.1 pilin -
  MARSALSMR5_RS07000 (MARSALSMR5_01432) - 1566989..1568482 (+) 1494 WP_198324914.1 O-antigen ligase -
  MARSALSMR5_RS07005 (MARSALSMR5_01433) - 1568583..1569026 (+) 444 WP_085679900.1 pilin -
  MARSALSMR5_RS07010 (MARSALSMR5_01434) cysQ 1569128..1569895 (+) 768 WP_085679903.1 3'(2'),5'-bisphosphate nucleotidase CysQ -
  MARSALSMR5_RS07015 (MARSALSMR5_01435) rfaH 1569892..1570383 (+) 492 WP_085679906.1 transcription/translation regulatory transformer protein RfaH -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62790.74 Da        Isoelectric Point: 4.9580

>NTDB_id=227237 MARSALSMR5_RS06985 WP_085679891.1 1564257..1565972(-) (pilB) [Marinobacter salarius strain SMR5]
MSTNKSNVTLTGLARRFVDDGLIDESTAKDAFLQASHNRIPLITYLVQNNLANSGGLAFSAAMEFGVSVLDLNAFMPEMM
PDKVVDEKLIRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAILVDDAKLREAIDKYLESHDTSMGDLDDA
DLEGVETEGGDQEDDAVAATSEVDDAPIVKYVNKMLLDAIRGGASDVHFEPYEKTYRVRYRTDGILKEMSRPSIKLAPKI
SARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEEQKQLYLDALE
QPQGMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPAEINLEGINQVNVNTRVGLGFAEALRAFLRQDPDIIMVGE
IRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPSFNIATSVSLIIAQRLGRRLCNSCRQPADIPKDVLLAE
GFTQEQIDTGFTLYRPKGCDKCNGGYKGRVGIYEVVKITDELANMIMEEASSIKIAKQAQAEGFPNLRQSALKKVIEGVT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=227237 MARSALSMR5_RS06985 WP_085679891.1 1564257..1565972(-) (pilB) [Marinobacter salarius strain SMR5]
ATGTCTACCAATAAAAGCAATGTAACGCTTACAGGCTTGGCGAGACGTTTCGTCGACGACGGGCTTATTGATGAATCCAC
CGCGAAAGATGCCTTTCTTCAAGCATCCCACAACCGCATTCCACTCATTACCTACCTGGTGCAGAACAACCTGGCGAATA
GCGGGGGGCTCGCCTTTTCCGCCGCCATGGAGTTTGGCGTTTCGGTTCTCGATCTCAACGCCTTTATGCCAGAGATGATG
CCCGATAAGGTCGTAGACGAAAAACTGATACGAAAACACAATGCCCTGCCGCTCTATAAACGTGGAAACCGGCTGTTTAT
TGCGGTCTCCGACCCGACCAACATCCAGGCGTTGGACGAAATCAAGTTTAATACCGGCCTGAGCACAGACGCCATCCTGG
TTGACGACGCCAAGCTCCGGGAAGCCATCGACAAGTATCTTGAGTCCCACGATACGTCGATGGGTGATCTGGATGACGCC
GACCTTGAAGGCGTTGAAACGGAAGGCGGCGACCAGGAAGACGATGCCGTTGCCGCGACGAGCGAAGTAGACGATGCCCC
CATCGTGAAGTACGTGAACAAGATGCTTCTTGACGCCATTCGCGGGGGGGCCTCGGACGTTCACTTTGAACCCTATGAGA
AAACCTATCGGGTTCGATACCGCACCGACGGCATTCTGAAAGAAATGTCGCGCCCCTCCATCAAGTTGGCCCCGAAGATT
TCTGCCCGGGTAAAAATCATGGCGCAGCTGGATATCTCGGAGCGCCGGGTCCCCCAGGACGGCCGGATCAAGATGAAGCT
GTCCAAGACCAAGGCTATCGACTTCCGGGTCAACACCCTCCCTACACTTTGGGGAGAAAAGATCGTTCTGCGGATTCTCG
ACCCAAGTCAGGCGAAGCTCGGCATCGATGTTCTGGGTTATGAGGAAGAACAGAAACAGCTGTACCTGGACGCCCTTGAG
CAGCCACAGGGAATGATACTGGTGACGGGACCCACCGGCTCGGGCAAGACAGTTTCCCTTTATACTGGCCTGAACATACT
GAACACACCAGAAAGGAACATCTCCACAGCCGAAGACCCTGCGGAAATCAACCTGGAAGGCATCAACCAGGTCAACGTCA
ATACCAGGGTGGGCCTCGGCTTTGCGGAAGCATTGAGAGCCTTTCTGCGTCAGGATCCGGACATAATCATGGTGGGTGAG
ATCCGAGACCTGGAAACGGCGAATATTGCTATCAAAGCGGCGCAAACAGGGCACCTGGTGCTCTCGACCCTTCACACCAA
CAGCGCTGCGGAAACGCTCACGCGCATGATGAACATGGGCGTTCCGTCCTTCAACATCGCCACGTCGGTGAGCCTGATTA
TCGCCCAGCGCCTGGGCCGCCGGCTTTGTAACTCTTGTAGACAACCTGCCGACATCCCCAAAGACGTGCTTTTAGCGGAG
GGGTTCACACAGGAACAAATTGATACAGGGTTCACGTTGTACCGCCCCAAGGGCTGTGATAAATGCAATGGTGGATATAA
AGGCCGCGTCGGGATTTACGAGGTGGTCAAGATTACCGACGAGCTGGCGAACATGATTATGGAAGAAGCCAGTTCGATTA
AAATTGCAAAGCAGGCACAGGCCGAAGGCTTCCCGAACTTGCGGCAGTCCGCCCTTAAAAAAGTCATTGAGGGCGTGACC
AGTCTTGAGGAAGCCAACCGCGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(21-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.894

100

0.571
  pilB Acinetobacter baylyi ADP1

56.643

100

0.567
  pilB Legionella pneumophila strain ERS1305867

55.124

99.124

0.546
  pilB Vibrio parahaemolyticus RIMD 2210633

52.065

97.548

0.508
  pilB Vibrio campbellii strain DS40M4

50.709

98.774

0.501
  pilB Vibrio cholerae strain A1552

53.846

91.068

0.49
  pilF Neisseria gonorrhoeae MS11

47.527

99.124

0.471
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.304

92.119

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

40.734

90.718

0.37

Multiple sequence alignment