Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VV1_RS07700 Genome accession   NC_004459
Coordinates   1592486..1594174 (-) Length   562 a.a.
NCBI ID   WP_011079550.1    Uniprot ID   A0A3Q0L402
Organism   Vibrio vulnificus CMCP6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1587486..1599174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VV1_RS07670 (VV1_1618) rplS 1588048..1588401 (+) 354 WP_011079544.1 50S ribosomal protein L19 -
  VV1_RS07675 (VV1_1619) yacG 1588609..1588803 (-) 195 WP_011079545.1 DNA gyrase inhibitor YacG -
  VV1_RS07680 (VV1_1620) zapD 1588866..1589606 (-) 741 WP_011079546.1 cell division protein ZapD -
  VV1_RS07685 (VV1_1622) coaE 1589639..1590247 (-) 609 WP_011079547.1 dephospho-CoA kinase -
  VV1_RS07690 (VV1_1623) pilD 1590249..1591118 (-) 870 WP_011079548.1 A24 family peptidase Machinery gene
  VV1_RS07695 (VV1_1624) pilC 1591212..1592438 (-) 1227 WP_011079549.1 type II secretion system F family protein Machinery gene
  VV1_RS07700 (VV1_1625) pilB 1592486..1594174 (-) 1689 WP_011079550.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VV1_RS07705 (VV1_1626) - 1594174..1594620 (-) 447 WP_011079551.1 pilin -
  VV1_RS07710 (VV1_1627) nadC 1594840..1595727 (-) 888 WP_011079552.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VV1_RS07715 (VV1_1628) ampD 1595820..1596392 (+) 573 WP_011079553.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VV1_RS07720 (VV1_1629) pdhR 1596781..1597548 (+) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62478.53 Da        Isoelectric Point: 5.6863

>NTDB_id=22543 VV1_RS07700 WP_011079550.1 1592486..1594174(-) (pilB) [Vibrio vulnificus CMCP6]
MMSNLSTILRQANQLSLTQEQACRETIQASGVTAPEALLRLGFFQPDELTEQLSAIFGLPRTDLNQYDYQELCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQSSGLKEIHQDELA
NLVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADKQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=22543 VV1_RS07700 WP_011079550.1 1592486..1594174(-) (pilB) [Vibrio vulnificus CMCP6]
ATGATGTCGAATCTCTCCACTATTCTGCGTCAGGCCAATCAGCTTAGCCTAACGCAAGAACAAGCGTGTCGAGAGACAAT
ACAAGCTTCTGGTGTGACAGCACCGGAAGCTTTGTTGCGACTGGGATTTTTCCAGCCGGATGAATTAACCGAGCAGCTAA
GCGCGATTTTTGGCTTACCACGCACTGACCTCAACCAATATGACTACCAAGAGCTGTGTAATCAACTTGGATTACGTGAG
CTAATCACCCGTCACCAAGCGCTGCCACTGCAAAAAAGCAACGCTGGTTTAATACTCGCGGTTGCAGACCCTACCAATAT
TCAAGCTGAAGATGATTTCCGTTTTGCCACAGGCGTGCAAGTGGAGATTGTTTTAGCCGATTGCCAAGAACTGCAAGCGG
CAATTCGTAGACTCTATGGAAAGAGCATCAAACAGAGCCAAAGCTCTGGGTTAAAAGAGATTCACCAAGATGAGCTGGCG
AATTTGGTCGAGGTTTCAGATGACGAAGTCGAGTCAATTGAAGACTTAAGCCAAGATGAATCCCCCGTTAGCCGCTTTAT
CAACCAAATCCTGCTGGATGCGGTACGCAAAGGCGCATCTGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTGC
GATTGCGTTGCGATGGTATTTTGGTGGAAGTGCAGCAGCCCCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAG
ATCCTCTCTAAGTTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGGATTAAGCTGCGCCTTAACCAAGAAACCGC
GATTGATATGCGTGTTTCCACCTTACCCACACTGTTTGGTGAAAAAATTGTGTTGCGACTTCTCGACAGCTCGAGCAGTA
GCTTGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAGCAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGAATG
ATTTTGATGACAGGCCCAACAGGAAGCGGTAAAACCGTTTCTCTGTACACAGGCTTGAGTTTACTTAACCGGCCAGAAGT
GAATATATCCACTGCGGAAGACCCAGTCGAGATTAACCTCCCGGGAATCAACCAAGTGCAAGTGAAACCCAAAATTGGTT
TTGGTTTTGCAGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCAGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAA
ACCGCCGAGATCGCCGTCAAAGCAGCCCAAACCGGCCACCTTGTACTATCCACCTTACATACCAACTCGGCTGCTGAAAC
CATTGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACTTAGCTTCATCCTTGAGTTTGATCATTGCTCAGCGTCTGG
CTCGTCGGTTATGCAGCCATTGCAAGCAACCTCATCCATACAACGCAACATTTGAGTTACTCGGTCTAAAAGCTGACGAC
TTGATTTATCAAGCTGATAAACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCAT
GCCTTTTGATGAGCAGCTTGCAGAGGCGATCGTTAAAGGCGCCTCGGTACAAAGCTTGGAGATGCTTGCTCGGCAAAAAG
GCATGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGCTTAGAAGAGCTGCAACGCGTCCTC
TATTTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L402

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

76.69

100

0.767
  pilB Vibrio parahaemolyticus RIMD 2210633

76.157

100

0.762
  pilB Vibrio cholerae strain A1552

74.423

100

0.746
  pilB Legionella pneumophila strain ERS1305867

52.827

91.281

0.482
  pilB Acinetobacter baumannii D1279779

51.533

92.883

0.479
  pilB Acinetobacter baylyi ADP1

50.383

92.883

0.468
  pilF Neisseria gonorrhoeae MS11

45.31

100

0.456
  pilF Thermus thermophilus HB27

40.608

99.466

0.404
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.302

94.306

0.361

Multiple sequence alignment