Detailed information NTG0224790

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: B7L46_RS07340
• Genome accession: NZ_CP020597
• Coordinates: 1324609..1325667 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain HWBA8
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1319609..1330667

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B7L46_RS07315 (B7L46_07320)	aroK	1319884..1320453 (-)	570	WP_000643712.1	shikimate kinase AroK	-
B7L46_RS07320 (B7L46_07325)	pilQ	1320465..1322630 (-)	2166	WP_001017029.1	type IV pilus secretin PilQ family protein	Machinery gene
B7L46_RS07325 (B7L46_07330)	pilP	1322693..1323220 (-)	528	WP_000695061.1	pilus assembly protein PilP	Machinery gene
B7L46_RS07330 (B7L46_07335)	pilO	1323231..1323971 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
B7L46_RS07335 (B7L46_07340)	pilN	1323968..1324609 (-)	642	WP_000201221.1	PilN domain-containing protein	Machinery gene
B7L46_RS07340 (B7L46_07345)	pilM	1324609..1325667 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
B7L46_RS07345 (B7L46_07350)	ponA	1325829..1328384 (+)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
B7L46_RS07350 (B7L46_07355)	-	1328402..1329226 (-)	825	WP_002019666.1	putative RNA methyltransferase	-
B7L46_RS07355 (B7L46_07360)	-	1329229..1329561 (-)	333	WP_000169599.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=224790 B7L46_RS07340 WP_002014066.1 1324609..1325667(-) (pilM) [Acinetobacter baumannii strain HWBA8]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=224790 B7L46_RS07340 WP_002014066.1 1324609..1325667(-) (pilM) [Acinetobacter baumannii strain HWBA8]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGACTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCAGAGCGAAAAAAGATCGTTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGCTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTATCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423