Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   B7D75_RS26140 Genome accession   NZ_CP020560
Coordinates   5527777..5529921 (-) Length   714 a.a.
NCBI ID   WP_053814716.1    Uniprot ID   -
Organism   Pseudomonas paraeruginosa strain Cr1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5522777..5534921
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7D75_RS26125 (B7D75_26140) - 5524438..5526084 (-) 1647 WP_053814715.1 AAA family ATPase -
  B7D75_RS26130 (B7D75_26145) aroB 5526091..5527197 (-) 1107 WP_012077719.1 3-dehydroquinate synthase -
  B7D75_RS26135 (B7D75_26150) aroK 5527247..5527765 (-) 519 WP_003148769.1 shikimate kinase AroK -
  B7D75_RS26140 (B7D75_26155) pilQ 5527777..5529921 (-) 2145 WP_053814716.1 type 4a pilus secretin PilQ Machinery gene
  B7D75_RS26145 (B7D75_26160) pilP 5529975..5530499 (-) 525 WP_034082521.1 type 4a pilus biogenesis lipoprotein PilP -
  B7D75_RS26150 (B7D75_26165) pilO 5530496..5531119 (-) 624 WP_024916549.1 type 4a pilus biogenesis protein PilO -
  B7D75_RS26155 (B7D75_26170) pilN 5531116..5531712 (-) 597 WP_003148777.1 type 4a pilus biogenesis protein PilN -
  B7D75_RS26160 (B7D75_26175) pilM 5531712..5532776 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 77296.87 Da        Isoelectric Point: 5.3150

>NTDB_id=224136 B7D75_RS26140 WP_053814716.1 5527777..5529921(-) (pilQ) [Pseudomonas paraeruginosa strain Cr1]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDQPVATPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTTLSSYTTRVEGNNLFVVVGNSPAGASVASAAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTADDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SEGGAEGKEGGRGSITVDDRTNSIIAYQPQDRLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHKGNW
NAYGKDGNMGIKDEEGLNCGPIAGNCSFPTVGTNKSPSPFVDIGAKDATSGIGIGFITDNIILDLQLSAMEKTGNGEIVS
QPKVVTSDKETAKILKGQEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDFDRALNGVPPINKN
EVNAKILVNDGETIVIGGVFSNTQSKSVDKVPFLGDLPYLGRLFRRDTVSDVKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=224136 B7D75_RS26140 WP_053814716.1 5527777..5529921(-) (pilQ) [Pseudomonas paraeruginosa strain Cr1]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCCTTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCGCTGCCCGGAGACCGGGTCGAGCTCAAGCTGCAGTTCGACCAGCCCGTCGCCACGCCGCGCG
GCTATACCATCGAGCAGCCGGCGCGGATCGCCCTCGACCTGCCGGGGGTGCAGAACAAGCTCGGCACCAAGAACCGCGAA
CTGAGCGTGGGCAATACACGCAGCGTCACGGTGGTCGAGGCGAAGGACCGCACACGGCTGATCATCAACCTGACCACGCT
GTCGTCCTACACCACCCGGGTGGAGGGCAACAACCTGTTCGTGGTGGTCGGCAATTCGCCGGCCGGCGCCAGCGTCGCTT
CCGCCGCTCCGGTCAAGGCGAGCCCGGCTCCGGCCAGCTACGCCCAGCCGATCAAGCCCAAGCCGTACGTGCCGGCGGGC
CGCGCGATCCGCAATATCGACTTCCAACGCGGCGAGAAGGGCGAGGGCAATGTGGTCATCGACCTCTCCGACCCGACGCT
GAGCCCGGATATCCAGGAGCAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAGCTGCCCGACGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACCCCGGTGCAGTTCGTCAACGCCAGTGCGCAATCCGACCGTACCAGCATCACCATC
GAGCCGAGCGGGCTCTACGACTACCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAGCCGATGACCGCGGA
CGACGCCGAGCGGCGCAAGAAGGACAACTTCGCCTACACCGGTGAGAAGCTGTCGCTGAACTTCCAGGACATCGACGTGC
GGTCGGTGCTGCAATTGATCGCCGACTTCACCGATCTGAATCTGGTGGCCTCGGATACCGTGCAGGGCAACATCACCCTG
CGCCTGCAGAATGTGCCGTGGGACCAGGCGCTGGACCTCGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTGCTGCTGGTGGCGCCGGCCGACGAGATCGCCGCACGCGAGCGTCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAAC
TGGCGCCGCTGCGTCGCGAGCTGATCCAGGTGAACTACGCCAAGGCGGCGGACATCGCCAAGCTGTTCCAGTCGGTGACC
AGCGAGGGCGGTGCGGAAGGCAAGGAAGGCGGGCGCGGCTCGATCACCGTGGACGACCGCACCAACAGCATCATCGCCTA
CCAGCCGCAAGATCGCCTGGATGAGCTGCGGCGGATCGTTTCGCAACTGGACATCCCGGTACGCCAGGTGATGATCGAGG
CGCGCATCGTCGAAGCCAACGTCGGCTACGACAAGAGCCTCGGCGTGCGCTGGGGCGGTGCCTACCACAAGGGCAACTGG
AATGCCTACGGCAAGGACGGCAATATGGGGATCAAGGACGAGGAAGGCCTGAACTGCGGTCCCATCGCCGGCAACTGTAG
CTTCCCCACCGTGGGCACCAACAAGTCGCCGTCGCCGTTTGTCGATATTGGCGCGAAGGATGCCACTTCCGGGATCGGTA
TCGGCTTCATCACCGACAACATCATCCTCGACCTGCAACTGTCGGCGATGGAAAAGACCGGCAACGGCGAGATCGTCTCC
CAGCCCAAGGTCGTGACCTCGGACAAGGAAACCGCGAAGATCCTCAAGGGCCAGGAAGTGCCGTACCAGGAGGCCAGCTC
CAGCGGTGCCACCAGTACCTCCTTCAAGGAGGCCGCACTGTCCCTCGAGGTGACCCCGCAGATCACGCCGGACAACCGGA
TCATCGTCGAGGTCAAGGTCACCAAGGACGCGCCGGACTTCGATCGTGCCCTGAACGGAGTACCGCCGATCAACAAGAAC
GAGGTCAATGCCAAGATCCTGGTCAATGACGGCGAGACCATCGTCATCGGCGGTGTATTCAGCAACACCCAGAGCAAGTC
GGTGGACAAGGTGCCGTTCCTCGGCGACCTGCCCTACCTGGGGCGGCTGTTCCGGCGTGATACGGTCTCGGACGTGAAAA
ACGAACTTCTGGTTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(549-705)

Secretin and TonB N terminus short domain (STN)

(307-353)

AMIN domain (AMIN)

(38-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

96.078

100

0.961
  comQ Acinetobacter baylyi ADP1

40.922

100

0.41
  pilQ Acinetobacter baumannii D1279779

40.839

100

0.409
  pilQ Acinetobacter baumannii strain A118

40.839

100

0.409
  pilQ Legionella pneumophila strain Lp02

37.374

97.059

0.363
  pilQ Legionella pneumophila strain ERS1305867

37.374

97.059

0.363

Multiple sequence alignment