Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   B7D75_RS26160 Genome accession   NZ_CP020560
Coordinates   5531712..5532776 (-) Length   354 a.a.
NCBI ID   WP_003110888.1    Uniprot ID   -
Organism   Pseudomonas paraeruginosa strain Cr1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5526712..5537776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7D75_RS26135 (B7D75_26150) aroK 5527247..5527765 (-) 519 WP_003148769.1 shikimate kinase AroK -
  B7D75_RS26140 (B7D75_26155) pilQ 5527777..5529921 (-) 2145 WP_053814716.1 type 4a pilus secretin PilQ Machinery gene
  B7D75_RS26145 (B7D75_26160) pilP 5529975..5530499 (-) 525 WP_034082521.1 type 4a pilus biogenesis lipoprotein PilP -
  B7D75_RS26150 (B7D75_26165) pilO 5530496..5531119 (-) 624 WP_024916549.1 type 4a pilus biogenesis protein PilO -
  B7D75_RS26155 (B7D75_26170) pilN 5531116..5531712 (-) 597 WP_003148777.1 type 4a pilus biogenesis protein PilN -
  B7D75_RS26160 (B7D75_26175) pilM 5531712..5532776 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  B7D75_RS26165 (B7D75_26180) - 5532961..5535432 (+) 2472 WP_012077723.1 penicillin-binding protein 1A -
  B7D75_RS26170 (B7D75_26185) - 5535537..5536805 (-) 1269 WP_107114570.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37983.48 Da        Isoelectric Point: 4.5405

>NTDB_id=224137 B7D75_RS26160 WP_003110888.1 5531712..5532776(-) (pilM) [Pseudomonas paraeruginosa strain Cr1]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=224137 B7D75_RS26160 WP_003110888.1 5531712..5532776(-) (pilM) [Pseudomonas paraeruginosa strain Cr1]
GTGCTAGGGCTCATAAAAAAGAAAGCGAATACCTTACTGGGGATCGACATCAGTTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAACGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAATCGGCCGTGGTG
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACGATCGAGATGGAGGCCGGGCTTTCCGAGGACGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAGGTCGCCATCGACTTCGAGGTCCAGGGTCTTTCCGCGC
GCAATCCCGAGCGCGTGGACGTGCTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTGCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGTTCCTATGCGCTGCTGAGCAGTCAGTTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTGGTGGACATCGGCGCGACCATGACAACGCTCAGCGTCCTGCACAACGGCC
GCACCATCTATACCCGCGAGCAGTTGTTCGGCGGACGCCAGCTCACCGAGGAGATCCAGCGGCGCTACGGGCTTTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGTGGTCTTCCGGATGACTACGACAGCGAAGTCCTGCGTCCGTTCAAGGACGC
CGTGGTCCAGCAGGTTTCCCGTTCGCTGCAGTTCTTCTTCGCCGCCGGCCAGTTCAATGACGTCGACTACATCGTCCTGG
CGGGCGGCACGGCGTCGATCCAGGACCTCGACCGGCTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAACGCCGGCGCCCTGGCCAGCGACGCGCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.899

100

0.562
  comM Acinetobacter nosocomialis M2

55.899

100

0.562
  comM Acinetobacter baylyi ADP1

51.977

100

0.52
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466

Multiple sequence alignment