Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SO_RS02010 Genome accession   NC_004347
Coordinates   431608..433317 (-) Length   569 a.a.
NCBI ID   WP_011070776.1    Uniprot ID   Q8EJP6
Organism   Shewanella oneidensis MR-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 426608..438317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SO_RS01980 (SO_0410) mutT 426745..427137 (-) 393 WP_011070770.1 8-oxo-dGTP diphosphatase MutT -
  SO_RS01985 (SO_0411) yacG 427225..427434 (-) 210 WP_011070771.1 DNA gyrase inhibitor YacG -
  SO_RS01990 (SO_0412) zapD 427495..428229 (-) 735 WP_011070772.1 cell division protein ZapD -
  SO_RS01995 (SO_0413) coaE 428222..428839 (-) 618 WP_011070773.1 dephospho-CoA kinase -
  SO_RS02000 (SO_0414) pilD 428993..429919 (-) 927 WP_011070774.1 prepilin peptidase Machinery gene
  SO_RS02005 (SO_0415) pilC 430085..431356 (-) 1272 WP_011070775.1 type II secretion system F family protein Machinery gene
  SO_RS02010 (SO_0416) pilB 431608..433317 (-) 1710 WP_011070776.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SO_RS02015 (SO_0417) - 433391..433807 (-) 417 WP_011070777.1 pilin -
  SO_RS02020 (SO_0419) nadC 434288..435169 (-) 882 WP_011070778.1 carboxylating nicotinate-nucleotide diphosphorylase -
  SO_RS02025 (SO_0421) ampD 435463..436008 (+) 546 WP_011070779.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  SO_RS02030 (SO_0422) ampE 436278..437129 (+) 852 WP_011070780.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62775.20 Da        Isoelectric Point: 6.0579

>NTDB_id=22394 SO_RS02010 WP_011070776.1 431608..433317(-) (pilB) [Shewanella oneidensis MR-1]
MPTTGLHLGLSTLFIRKGLLNEEQMATAITKSRQNKQTLVTTLVQTKLVSARSIAELCYEEYGTPLLDLAEFDVSAIPEE
FLNKKLIEKHRCLPLFKRGNRLYIATSDPTNIAALEEFQFSAGLHAEAILVEEDKLAKALEKVLEEDITGLDLSGMDEAA
LAGIEITDTDKRNEETTGESSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLAGRLSA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEPDQEKLYLEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPECKQPEQIPDHELQRLGF
SDVQIAQGFTTYKPVGCEHCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIAGQAKAQGMRDLRQSGLLKVLQGVTSI
AEVNRVTSF

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=22394 SO_RS02010 WP_011070776.1 431608..433317(-) (pilB) [Shewanella oneidensis MR-1]
ATGCCCACCACAGGCTTACATTTAGGTTTATCCACTTTATTTATTCGTAAAGGGCTGCTAAATGAAGAGCAGATGGCGAC
TGCCATCACCAAATCCCGCCAAAACAAACAAACCTTAGTTACCACCTTAGTGCAGACTAAGCTCGTATCGGCCCGCAGTA
TTGCCGAGCTTTGCTATGAGGAATATGGCACGCCGCTGCTGGATTTAGCCGAATTTGATGTTAGCGCCATCCCTGAAGAG
TTTCTGAATAAAAAACTGATTGAAAAACACCGCTGCTTACCGCTATTTAAACGCGGCAATCGCCTGTATATCGCCACCTC
CGACCCCACCAATATTGCAGCCCTTGAAGAGTTCCAATTTAGTGCCGGCCTGCATGCCGAAGCGATTTTGGTGGAGGAGG
ATAAGCTCGCCAAAGCCCTTGAAAAAGTACTAGAAGAAGATATTACCGGCCTTGATTTAAGCGGCATGGATGAGGCGGCG
CTAGCGGGTATCGAAATTACCGATACCGATAAACGCAATGAAGAGACAACTGGCGAAAGTAGTGATGATGCGCCAATTGT
TATCTATATCAATAAGATATTAACCGACGCCATTCGCAAGGGCGCCTCAGACTTACATTTTGAGCCCTATGAAAAACGCT
ATCGTATCCGTTTTCGTATCGATGGCATTTTGCATGAAGTCTCCGAACCGCCCATTAGCTTAGCTGGACGACTCTCGGCG
CGGTTAAAGGTGATGTCAAAGCTCGATATTGCCGAGCGCCGCGTCCCTCAAGATGGCCGGATTAAGATGAAGCTGTCCCG
CACTAAGTCCATCGACTTTCGGGTAAGCACCCTGCCGACCCTGTGGGGCGAAAAAATTGTAATGCGGATATTGGACTCGT
CCTCCGCCCAATTAGGCATTGAAAAACTAGGCTACGAGCCTGATCAAGAAAAACTCTACCTTGAGATGCTGGCCAAACCC
CAAGGGATGATTCTGGTCACAGGCCCCACGGGTTCGGGTAAAACCGTTTCGCTCTATACAGGGTTAAATATCCTCAATAC
CGCCGAGCGTAATATCTCCACCGCTGAAGATCCGGTTGAGATTAACCTTGAGGGCGTAAACCAAGTTCACATTAATTTAA
AGGCCGGTTTAACCTTTGCCTCGGCACTACGCTCCTTTTTGCGCCAAGACCCCGATGTGGTAATGGTGGGCGAAATCCGC
GACCTTGAAACCGCCGAAATTGCCATTAAAGCCGCGCAAACTGGCCACTTAGTATTATCAACTCTGCATACCAACTCCGC
CGCCGAAACCTTAACCCGCTTAATCAATATGGGCGTGCCTGGCTATAATATCGCCAGCTCAGTTAACTTAATTATTGCCC
AGCGTTTGGCACGGCGGCTCTGCCCCGAATGTAAACAGCCCGAGCAAATACCTGATCATGAGTTACAACGTTTAGGCTTT
AGCGATGTGCAAATCGCCCAAGGTTTTACCACCTATAAACCCGTGGGCTGCGAGCATTGCTCCGGTGGCTATAAAGGACG
GGTGGGGATTTACGAAGTGATGAAGATGTCAGATGAAATTGCCCGTACTATTATGGAAGGGGGCAACTCGTTACAGATTG
CTGGCCAAGCTAAAGCGCAAGGCATGCGCGATCTGCGTCAATCGGGGCTACTAAAAGTACTCCAAGGCGTGACCAGTATT
GCCGAAGTGAATCGGGTTACCAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8EJP6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.358

98.594

0.585
  pilB Acinetobacter baumannii D1279779

59.45

95.782

0.569
  pilB Legionella pneumophila strain ERS1305867

56.584

98.77

0.559
  pilB Vibrio cholerae strain A1552

53.333

97.54

0.52
  pilB Vibrio parahaemolyticus RIMD 2210633

51.489

100

0.517
  pilB Vibrio campbellii strain DS40M4

51.237

99.473

0.51
  pilF Neisseria gonorrhoeae MS11

48.506

100

0.485
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.427

98.067

0.387
  pilF Thermus thermophilus HB27

38.859

98.594

0.383
  pilB/pilB1 Synechocystis sp. PCC 6803

41.036

88.225

0.362

Multiple sequence alignment