Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA/cilB/dalA   Type   Machinery gene
Locus tag   A6J72_RS00665 Genome accession   NZ_CP020433
Coordinates   120802..121644 (+) Length   280 a.a.
NCBI ID   WP_082311502.1    Uniprot ID   -
Organism   Streptococcus intermedius strain FDAARGOS_233     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 115802..126644
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6J72_RS00615 (A6J72_00620) - 116796..117146 (+) 351 WP_082312340.1 hypothetical protein -
  A6J72_RS00620 (A6J72_00625) - 117166..117516 (+) 351 WP_082311496.1 hypothetical protein -
  A6J72_RS00625 (A6J72_00630) - 117753..117944 (+) 192 WP_082311497.1 hypothetical protein -
  A6J72_RS00630 (A6J72_00635) - 117931..118281 (+) 351 WP_082311498.1 hypothetical protein -
  A6J72_RS00635 (A6J72_00640) - 118301..118651 (+) 351 WP_082311499.1 hypothetical protein -
  A6J72_RS00640 (A6J72_09610) - 118713..119405 (+) 693 WP_225791032.1 TNT domain-containing protein -
  A6J72_RS00645 (A6J72_00650) - 119402..119737 (+) 336 WP_082311500.1 Imm59 family immunity protein -
  A6J72_RS00650 (A6J72_00655) - 119730..120065 (+) 336 WP_082311501.1 Imm59 family immunity protein -
  A6J72_RS00655 (A6J72_00660) - 120069..120410 (+) 342 WP_003072664.1 Imm59 family immunity protein -
  A6J72_RS00665 (A6J72_00665) dprA/cilB/dalA 120802..121644 (+) 843 WP_082311502.1 DNA-processing protein DprA Machinery gene
  A6J72_RS00670 (A6J72_00670) topA 121730..123823 (+) 2094 WP_105255902.1 type I DNA topoisomerase -
  A6J72_RS00675 (A6J72_00675) - 123879..124373 (+) 495 WP_082311504.1 phosphoribosylanthranilate isomerase -
  A6J72_RS00680 (A6J72_00680) trmFO 124467..125801 (+) 1335 WP_082311505.1 methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO -

Sequence

Protein


Download         Length: 280 a.a.        Molecular weight: 31217.74 Da        Isoelectric Point: 5.4100

>NTDB_id=222973 A6J72_RS00665 WP_082311502.1 120802..121644(+) (dprA/cilB/dalA) [Streptococcus intermedius strain FDAARGOS_233]
MNNFDIYKLKEAGLTNQQVINVLEYAEIHEKELSAKDMAVVSECRNPALFIEKYLQLDDDLLRQEFEKFPSFSILDDVYP
WDLSEIYNPPVLLFYKGNLDLLKLPKVAVVGSRNSSRVGSQSVQKIIKELNNELVIVSGLARGIDTSAHMAALQNGGRTI
AVIGTGLDVFYPRANKKLQSYLGEHHLVLSEYSPEAEPLKFHFPERNRIIAGLCRGVIVAEAKMRSGSLITCERAMEEGR
DVFAIPGSILDGRSDGCHHLIQEGAKCITSGSDVLSEFQF

Nucleotide


Download         Length: 843 bp        

>NTDB_id=222973 A6J72_RS00665 WP_082311502.1 120802..121644(+) (dprA/cilB/dalA) [Streptococcus intermedius strain FDAARGOS_233]
ATGAATAATTTTGATATTTATAAATTAAAAGAAGCCGGCTTGACAAACCAACAAGTTATCAATGTATTAGAATATGCTGA
AATACATGAAAAAGAATTGTCTGCTAAAGACATGGCAGTTGTTTCCGAATGCCGTAATCCTGCTCTTTTTATTGAAAAAT
ATTTGCAGTTAGATGATGATTTATTACGTCAAGAATTTGAGAAATTCCCATCCTTTTCTATCTTGGACGATGTATATCCT
TGGGATTTGAGTGAAATTTATAATCCACCCGTTCTTCTTTTTTATAAAGGTAATTTGGATTTGCTAAAATTACCTAAAGT
GGCTGTTGTAGGAAGTCGAAATAGTAGCCGAGTAGGCAGTCAATCTGTTCAAAAGATTATTAAAGAGCTAAACAATGAAT
TGGTTATCGTGAGTGGTTTAGCTCGCGGAATTGATACCTCGGCTCACATGGCAGCGTTGCAAAATGGTGGTCGAACAATT
GCAGTTATCGGAACGGGCCTAGATGTGTTTTACCCAAGGGCGAATAAAAAACTCCAATCGTATTTGGGAGAACATCATCT
AGTGCTTAGTGAGTATAGTCCTGAAGCAGAGCCATTAAAATTTCATTTTCCAGAGCGTAATCGGATTATAGCAGGATTGT
GTAGAGGGGTTATTGTTGCTGAAGCTAAAATGCGCTCAGGCAGCCTTATTACTTGTGAGCGAGCAATGGAAGAAGGGCGA
GATGTATTTGCTATTCCAGGGTCAATTTTAGATGGAAGAAGTGATGGCTGTCATCATTTGATTCAGGAGGGAGCAAAATG
TATCACATCAGGGTCTGACGTACTTTCTGAATTCCAGTTTTAA

Domains

Predicted by InterproScan.

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(1-60)

DNA recombination-mediator protein A (DNA_processg_A)

(73-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

76.786

100

0.768
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

76.429

100

0.764
  dprA/cilB/dalA Streptococcus pneumoniae D39

76.429

100

0.764
  dprA/cilB/dalA Streptococcus pneumoniae R6

76.429

100

0.764
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

76.429

100

0.764
  dprA/cilB/dalA Streptococcus mitis SK321

76.071

100

0.761
  dprA Streptococcus mutans UA159

65.357

100

0.654
  dprA Lactococcus lactis subsp. cremoris KW2

57.5

100

0.575
  dprA Legionella pneumophila strain ERS1305867

42.188

91.429

0.386
  dprA Staphylococcus aureus N315

37.589

100

0.379
  dprA Bacillus subtilis subsp. subtilis str. 168

48.095

75

0.361

Multiple sequence alignment