Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   A6J57_RS06955 Genome accession   NZ_CP020405
Coordinates   1471011..1471445 (+) Length   144 a.a.
NCBI ID   WP_005741096.1    Uniprot ID   -
Organism   Pasteurella multocida strain FDAARGOS_218     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1466011..1476445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6J57_RS06925 (A6J57_06910) tadA 1466121..1466621 (-) 501 WP_005755875.1 tRNA adenosine(34) deaminase TadA -
  A6J57_RS06930 (A6J57_06915) - 1466636..1467487 (-) 852 WP_005751072.1 thymidylate synthase -
  A6J57_RS06935 (A6J57_06920) lgt 1467493..1468296 (-) 804 WP_005755876.1 prolipoprotein diacylglyceryl transferase -
  A6J57_RS06940 (A6J57_06925) - 1468306..1469100 (-) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  A6J57_RS06945 (A6J57_06930) rppH 1469103..1469699 (-) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  A6J57_RS06950 (A6J57_06935) ampD 1470285..1470851 (-) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  A6J57_RS06955 (A6J57_06940) pilA 1471011..1471445 (+) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  A6J57_RS06960 (A6J57_06945) pilB 1471445..1472830 (+) 1386 WP_005755879.1 GspE/PulE family protein Machinery gene
  A6J57_RS06965 (A6J57_06950) pilC 1472830..1474041 (+) 1212 WP_005755881.1 type II secretion system F family protein Machinery gene
  A6J57_RS06970 (A6J57_06955) - 1474041..1474763 (+) 723 WP_005755882.1 A24 family peptidase -
  A6J57_RS06975 (A6J57_06960) coaE 1474778..1475398 (+) 621 WP_005751078.1 dephospho-CoA kinase -
  A6J57_RS06980 (A6J57_06965) yacG 1475409..1475612 (+) 204 WP_005755884.1 DNA gyrase inhibitor YacG -
  A6J57_RS06985 (A6J57_06970) - 1475612..1475881 (+) 270 WP_005755886.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15058.48 Da        Isoelectric Point: 9.1281

>NTDB_id=222278 A6J57_RS06955 WP_005741096.1 1471011..1471445(+) (pilA) [Pasteurella multocida strain FDAARGOS_218]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=222278 A6J57_RS06955 WP_005741096.1 1471011..1471445(+) (pilA) [Pasteurella multocida strain FDAARGOS_218]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCAGCTC
CTTATCGTGCTGAGGTAGAGCTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.806

100

0.368
  pilA Vibrio cholerae strain A1552

36.806

100

0.368
  pilA Vibrio cholerae C6706

36.806

100

0.368

Multiple sequence alignment