Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   A6J55_RS09120 Genome accession   NZ_CP020403
Coordinates   1932350..1932784 (+) Length   144 a.a.
NCBI ID   WP_005741096.1    Uniprot ID   -
Organism   Pasteurella multocida strain FDAARGOS_216     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1927350..1937784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6J55_RS09090 (A6J55_09090) tadA 1927460..1927960 (-) 501 WP_005753343.1 tRNA adenosine(34) deaminase TadA -
  A6J55_RS09095 (A6J55_09095) - 1927975..1928826 (-) 852 WP_005753345.1 thymidylate synthase -
  A6J55_RS09100 (A6J55_09100) lgt 1928832..1929635 (-) 804 WP_005753347.1 prolipoprotein diacylglyceryl transferase -
  A6J55_RS09105 (A6J55_09105) - 1929645..1930439 (-) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  A6J55_RS09110 (A6J55_09110) rppH 1930442..1931038 (-) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  A6J55_RS09115 (A6J55_09115) ampD 1931624..1932190 (-) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  A6J55_RS09120 (A6J55_09120) pilA 1932350..1932784 (+) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  A6J55_RS09125 (A6J55_09125) pilB 1932784..1934169 (+) 1386 WP_005753352.1 GspE/PulE family protein Machinery gene
  A6J55_RS09130 (A6J55_09130) - 1934169..1935380 (+) 1212 WP_005753354.1 type II secretion system F family protein -
  A6J55_RS09135 (A6J55_09135) - 1935380..1936102 (+) 723 WP_032851624.1 A24 family peptidase -
  A6J55_RS09140 (A6J55_09140) coaE 1936117..1936737 (+) 621 WP_005753357.1 dephospho-CoA kinase -
  A6J55_RS09145 (A6J55_09145) yacG 1936748..1936951 (+) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  A6J55_RS09150 (A6J55_09150) - 1936951..1937220 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15058.48 Da        Isoelectric Point: 9.1281

>NTDB_id=222258 A6J55_RS09120 WP_005741096.1 1932350..1932784(+) (pilA) [Pasteurella multocida strain FDAARGOS_216]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=222258 A6J55_RS09120 WP_005741096.1 1932350..1932784(+) (pilA) [Pasteurella multocida strain FDAARGOS_216]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCTGAGGTAGAGCTTTGTATCTATAATACAAATAGTACAACGAATTGCAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTGAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.806

100

0.368
  pilA Vibrio cholerae strain A1552

36.806

100

0.368
  pilA Vibrio cholerae C6706

36.806

100

0.368

Multiple sequence alignment