Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A6J55_RS09125 Genome accession   NZ_CP020403
Coordinates   1932784..1934169 (+) Length   461 a.a.
NCBI ID   WP_005753352.1    Uniprot ID   -
Organism   Pasteurella multocida strain FDAARGOS_216     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1927784..1939169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6J55_RS09095 (A6J55_09095) - 1927975..1928826 (-) 852 WP_005753345.1 thymidylate synthase -
  A6J55_RS09100 (A6J55_09100) lgt 1928832..1929635 (-) 804 WP_005753347.1 prolipoprotein diacylglyceryl transferase -
  A6J55_RS09105 (A6J55_09105) - 1929645..1930439 (-) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  A6J55_RS09110 (A6J55_09110) rppH 1930442..1931038 (-) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  A6J55_RS09115 (A6J55_09115) ampD 1931624..1932190 (-) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  A6J55_RS09120 (A6J55_09120) pilA 1932350..1932784 (+) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  A6J55_RS09125 (A6J55_09125) pilB 1932784..1934169 (+) 1386 WP_005753352.1 GspE/PulE family protein Machinery gene
  A6J55_RS09130 (A6J55_09130) - 1934169..1935380 (+) 1212 WP_005753354.1 type II secretion system F family protein -
  A6J55_RS09135 (A6J55_09135) - 1935380..1936102 (+) 723 WP_032851624.1 A24 family peptidase -
  A6J55_RS09140 (A6J55_09140) coaE 1936117..1936737 (+) 621 WP_005753357.1 dephospho-CoA kinase -
  A6J55_RS09145 (A6J55_09145) yacG 1936748..1936951 (+) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  A6J55_RS09150 (A6J55_09150) - 1936951..1937220 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  A6J55_RS09155 (A6J55_09155) - 1937669..1939042 (+) 1374 WP_005722571.1 sodium:alanine symporter family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52023.63 Da        Isoelectric Point: 6.2343

>NTDB_id=222259 A6J55_RS09125 WP_005753352.1 1932784..1934169(+) (pilB) [Pasteurella multocida strain FDAARGOS_216]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCSV
ETQSACCCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=222259 A6J55_RS09125 WP_005753352.1 1932784..1934169(+) (pilB) [Pasteurella multocida strain FDAARGOS_216]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGTGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGGATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTGCAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAGCCACAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCTCCCTCTGCTCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCAGCGTA
GAGACACAATCGGCGTGTTGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.212

98.265

0.67
  pilB Haemophilus influenzae Rd KW20

67.55

98.265

0.664
  pilB Glaesserella parasuis strain SC1401

58.37

98.482

0.575
  pilB Acinetobacter baumannii D1279779

39.959

100

0.427
  pilB Vibrio campbellii strain DS40M4

39.419

100

0.412
  pilB Acinetobacter baylyi ADP1

37.876

100

0.41
  pilB Vibrio parahaemolyticus RIMD 2210633

39.293

100

0.41
  pilB Vibrio cholerae strain A1552

38.683

100

0.408
  pilB Legionella pneumophila strain ERS1305867

37.219

100

0.395

Multiple sequence alignment