Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CXV92_RS11600 Genome accession   NZ_CP025859
Coordinates   2326348..2327829 (+) Length   493 a.a.
NCBI ID   WP_126130410.1    Uniprot ID   -
Organism   Escherichia coli strain 504239     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2321348..2332829
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CXV92_RS11580 gspB 2321517..2321936 (-) 420 WP_000461450.1 putative general secretion pathway protein GspB -
  CXV92_RS11585 gspA 2321938..2323407 (-) 1470 WP_000107576.1 type II secretion system protein GspA -
  CXV92_RS11590 gspC 2323587..2324402 (+) 816 WP_001142708.1 type II secretion system protein GspC -
  CXV92_RS11595 gspD 2324386..2326338 (+) 1953 WP_001326512.1 type II secretion system secretin GspD -
  CXV92_RS11600 pilB 2326348..2327829 (+) 1482 WP_126130410.1 type II secretion system ATPase GspE Machinery gene
  CXV92_RS11605 gspF 2327826..2329022 (+) 1197 WP_001109570.1 type II secretion system inner membrane protein GspF -
  CXV92_RS11610 gspG 2329032..2329469 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  CXV92_RS11615 gspH 2329477..2329986 (+) 510 WP_001076048.1 type II secretion system minor pseudopilin GspH -
  CXV92_RS11620 gspI 2329983..2330360 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  CXV92_RS11625 gspJ 2330353..2330940 (+) 588 WP_000610633.1 type II secretion system minor pseudopilin GspJ -
  CXV92_RS11630 gspK 2330933..2331916 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54684.56 Da        Isoelectric Point: 5.9498

>NTDB_id=220429 CXV92_RS11600 WP_126130410.1 2326348..2327829(+) (pilB) [Escherichia coli strain 504239]
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQITVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
YHQLAVTTIDTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHNALIKNGLQKVISGDTSWDE
VMRVASATLESEA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=220429 CXV92_RS11600 WP_126130410.1 2326348..2327829(+) (pilB) [Escherichia coli strain 504239]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGTCAGTGCCAGACCATGACCAGTTTGT
CACAGGCTGATTTCGAAGCCCGGCTGGAAGCGGTGTTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAGCAGGATTTAGAAAATCTCATTCAGCTT
CCGCACGGTATTATCTTGGTGACAGGGCCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATCCTTTCGGCGCTGAA
TACTCCCGGCCGCAATATTCTGACGGTAGAAGATCCCGTGGAATATGAGCTGGAAGGCATTGGGCAAACGCAGGTGAATA
CCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATT
CGTGATACAGAAACCGCGCAGATTACGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTGACCCGGCTCCGCGACATGGGCGTCGAATCATTCCTGCTTTCGTCTTCCCTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGCCTGTGTCCGCAATGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAA
TATCATCAGCTCGCGGTGACAACAATTGACACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTAGTCCGGCAACAACACAATGCCTTAATCAAAAATGGCCTGCAAAAAGTGATAAGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAGCGAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.453

88.641

0.412
  pilB Acinetobacter baumannii D1279779

45.578

89.452

0.408
  pilB Vibrio cholerae strain A1552

48.201

84.584

0.408
  pilB Legionella pneumophila strain ERS1305867

41.164

97.566

0.402
  pilB Vibrio parahaemolyticus RIMD 2210633

46.353

86.207

0.4
  pilB/pilB1 Synechocystis sp. PCC 6803

40.756

96.552

0.394
  pilB Acinetobacter baylyi ADP1

48.338

79.31

0.383

Multiple sequence alignment