Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CPS_RS19930 Genome accession   NC_003910
Coordinates   4683825..4685525 (+) Length   566 a.a.
NCBI ID   WP_011045176.1    Uniprot ID   Q47VS7
Organism   Colwellia psychrerythraea 34H     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4678825..4690525
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPS_RS19900 (CPS_4440) petA 4678833..4679423 (-) 591 WP_011045169.1 ubiquinol-cytochrome c reductase iron-sulfur subunit -
  CPS_RS23970 (CPS_4442) rpsI 4679881..4680273 (-) 393 WP_011045171.1 30S ribosomal protein S9 -
  CPS_RS23975 (CPS_4443) rplM 4680277..4680705 (-) 429 WP_011045172.1 50S ribosomal protein L13 -
  CPS_RS19915 (CPS_4444) - 4681376..4681858 (+) 483 WP_011045173.1 pilin -
  CPS_RS23035 (CPS_4445) - 4681986..4682684 (+) 699 WP_049757942.1 hypothetical protein -
  CPS_RS19925 (CPS_4446) - 4682776..4683486 (-) 711 WP_011045175.1 transposase -
  CPS_RS19930 (CPS_4447) pilB 4683825..4685525 (+) 1701 WP_011045176.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CPS_RS19935 (CPS_4448) pilC 4685644..4686903 (+) 1260 WP_011045177.1 type II secretion system F family protein Machinery gene
  CPS_RS19940 (CPS_4449) pilD 4687011..4687916 (+) 906 WP_011045178.1 prepilin peptidase Machinery gene
  CPS_RS19945 (CPS_4450) coaE 4688234..4688842 (+) 609 WP_011045179.1 dephospho-CoA kinase -
  CPS_RS19950 (CPS_4451) zapD 4688903..4689655 (+) 753 WP_011045180.1 cell division protein ZapD -
  CPS_RS19955 (CPS_4452) yacG 4689764..4690000 (+) 237 WP_011045181.1 DNA gyrase inhibitor YacG -
  CPS_RS19960 (CPS_4453) mutT 4690027..4690461 (-) 435 WP_011045182.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62190.64 Da        Isoelectric Point: 5.2329

>NTDB_id=22022 CPS_RS19930 WP_011045176.1 4683825..4685525(+) (pilB) [Colwellia psychrerythraea 34H]
MKGNHQQASLLSALSKQNLIPLESIDDITADFIGKKIPFIRFLIEDKSIDGNSIAKVLSKSFGHPLIQLSNFDTSLVPEG
VRNEKLITKHNALPLYLRGKVLFIAMSDPTNLDALEEIQFNTGYSTELVLCDEKSLQTCIEKVLEDESAALDISDIDADE
LAGIDVEESRKEDANANGEDDAPIVVFINKILLDAIKKGASDLHFEPYEKSFRIRFRIDGILSEIARPPVSLSSRMAARL
KVMSKLDIAERRVPQDGRIKLALSKKKSIDFRVSTLPTMWGEKVVMRILDSSSAMLGIDMLGYEPAQKKIYMEALEQPQG
MILVTGPTGSGKTVSLYTGLNILNTQERNISTAEDPVEINLEGINQVQINNRAGLTFPSALRSFLRQDPDIVMVGEIRDL
ETAEISIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPSYNVASSVSIIIAQRLARRLCPQCKEEEPLSEMLLAEQGFPA
DKLADIKLFKPIGCTHCTGGYKGRVGIYEVIKISPTIASIIMEGGNSLDIAKQCQKEGYNNLRQSGLLKAMSGMTSLEEI
NRVTSA

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=22022 CPS_RS19930 WP_011045176.1 4683825..4685525(+) (pilB) [Colwellia psychrerythraea 34H]
ATGAAAGGAAATCACCAACAAGCTAGTCTGCTATCGGCATTATCAAAGCAGAACCTTATTCCTTTAGAATCAATAGATGA
TATTACGGCTGATTTCATCGGTAAAAAAATACCTTTCATTCGTTTTTTGATTGAAGACAAAAGTATTGATGGAAATAGTA
TTGCTAAAGTACTTTCTAAAAGCTTTGGCCACCCACTAATTCAACTTAGTAACTTCGATACCAGCTTGGTACCTGAAGGT
GTTCGTAATGAAAAACTGATCACTAAACATAATGCATTGCCACTATATTTACGTGGCAAAGTACTGTTTATTGCCATGTC
AGACCCGACTAATTTAGATGCCTTAGAAGAAATACAGTTTAATACCGGTTACAGCACTGAGCTAGTATTATGTGATGAGA
AGAGCTTACAAACTTGTATTGAAAAAGTACTCGAAGATGAAAGTGCTGCATTAGATATAAGTGACATCGATGCAGATGAG
TTAGCGGGTATTGATGTTGAAGAGTCAAGAAAAGAAGACGCTAACGCTAACGGTGAAGATGATGCACCTATCGTTGTTTT
CATCAATAAAATATTACTCGATGCTATAAAGAAAGGGGCTTCAGATTTACATTTTGAACCTTATGAAAAGTCATTTCGTA
TTCGCTTTCGTATTGATGGTATTTTATCTGAAATAGCTAGACCACCAGTAAGCCTCTCTTCAAGAATGGCAGCCCGCTTG
AAAGTGATGTCTAAATTGGATATTGCCGAACGTCGTGTTCCACAAGATGGTCGAATTAAGTTGGCACTATCCAAGAAAAA
GTCGATCGATTTTCGTGTCAGTACTTTACCCACCATGTGGGGTGAAAAAGTCGTAATGCGCATATTAGATTCATCAAGTG
CTATGCTGGGTATAGATATGCTGGGTTATGAACCCGCTCAGAAAAAAATATACATGGAAGCATTAGAACAACCGCAAGGT
ATGATTTTAGTTACTGGACCAACAGGTTCAGGTAAAACGGTATCGCTTTATACCGGATTAAATATATTAAACACACAAGA
ACGTAATATATCTACAGCGGAAGACCCTGTCGAAATTAACCTTGAAGGCATCAACCAAGTACAAATCAATAATCGTGCCG
GTTTAACTTTCCCAAGTGCATTGCGCTCGTTTTTACGACAAGATCCAGATATTGTCATGGTGGGGGAAATTCGGGATTTA
GAAACCGCTGAAATTTCGATAAAAGCCGCACAAACTGGCCACTTAGTATTATCAACCCTGCATACTAACTCTGCTGCTGA
AACGCTTACTCGTTTACTTAATATGGGGGTTCCTTCCTATAATGTTGCCAGCTCTGTCTCTATTATTATTGCTCAACGTT
TGGCAAGACGATTATGCCCGCAGTGTAAGGAGGAAGAGCCTCTTTCTGAAATGCTATTAGCCGAACAAGGTTTTCCTGCT
GATAAATTAGCTGACATAAAGTTATTTAAACCTATAGGCTGTACTCATTGCACTGGAGGCTATAAAGGCCGCGTGGGTAT
TTACGAAGTGATTAAAATTAGCCCGACTATTGCTTCTATTATCATGGAAGGTGGCAACTCTCTTGATATAGCGAAGCAAT
GCCAAAAGGAGGGGTATAACAACTTGCGTCAATCAGGTTTATTAAAAGCAATGAGCGGTATGACAAGTTTAGAAGAAATT
AACCGAGTAACTAGCGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q47VS7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.357

93.463

0.555
  pilB Acinetobacter baumannii D1279779

59.091

93.286

0.551
  pilB Legionella pneumophila strain ERS1305867

55.784

94.7

0.528
  pilB Vibrio cholerae strain A1552

52.406

99.117

0.519
  pilB Vibrio campbellii strain DS40M4

52.632

93.993

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

53.981

90.989

0.491
  pilF Neisseria gonorrhoeae MS11

50

95.406

0.477
  pilB/pilB1 Synechocystis sp. PCC 6803

40.8

88.339

0.36

Multiple sequence alignment