Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BV121_RS01635 Genome accession   NZ_CP020008
Coordinates   338750..339199 (-) Length   149 a.a.
NCBI ID   WP_105182544.1    Uniprot ID   -
Organism   Haemophilus influenzae strain 5P28H1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 333750..344199
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BV121_RS01615 (BV121_328) rho 334140..335402 (+) 1263 WP_005648966.1 transcription termination factor Rho -
  BV121_RS01620 (BV121_329) pilD 335456..336148 (-) 693 WP_014550482.1 prepilin peptidase Machinery gene
  BV121_RS01625 (BV121_330) pilC 336145..337362 (-) 1218 WP_105182543.1 type II secretion system F family protein Machinery gene
  BV121_RS01630 (BV121_331) pilB 337359..338753 (-) 1395 WP_011271972.1 GspE/PulE family protein Machinery gene
  BV121_RS01635 (BV121_332) pilA 338750..339199 (-) 450 WP_105182544.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  BV121_RS01640 (BV121_333) ampD 339314..339874 (+) 561 WP_042599472.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BV121_RS01645 (BV121_334) corC 340487..341386 (+) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -
  BV121_RS01650 (BV121_335) lnt 341409..342938 (+) 1530 WP_079989936.1 apolipoprotein N-acyltransferase -
  BV121_RS01655 (BV121_336) rsmE 342988..343725 (+) 738 WP_011271976.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15454.80 Da        Isoelectric Point: 8.7944

>NTDB_id=219827 BV121_RS01635 WP_105182544.1 338750..339199(-) (pilA) [Haemophilus influenzae strain 5P28H1]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQHYTKKAAVSELLQASAPYKADVELCVYSTGKPSSCSGGSNGIA
ADIKTAKGYVKSVTTSNGAITVKGDGTLANMEYILQATGNAATGVTWTTTCKGTDASLFSANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=219827 BV121_RS01635 WP_105182544.1 338750..339199(-) (pilA) [Haemophilus influenzae strain 5P28H1]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGGTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCCTCTTATCAACATTATACTAAAAAAGCAGCGGTATCTGAATTACTGCAAGCGTCAGCGC
CTTATAAGGCTGATGTGGAATTATGTGTTTATAGCACAGGTAAACCTTCCAGTTGCTCGGGAGGAAGCAATGGAATTGCA
GCGGATATAAAGACAGCAAAAGGCTATGTAAAATCAGTGACAACAAGCAACGGTGCAATAACAGTAAAAGGGGATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTACAGGTAATGCTGCAACAGGTGTAACTTGGACAACAACTTGCAAAGGAA
CGGATGCCTCTTTATTTTCAGCAAATTTTTGCGGAAGTGTCACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

93.289

100

0.933
  pilA Haemophilus influenzae Rd KW20

87.919

100

0.879
  pilA Glaesserella parasuis strain SC1401

55.705

100

0.557
  pilA2 Legionella pneumophila str. Paris

40.441

91.275

0.369
  pilA2 Legionella pneumophila strain ERS1305867

40.441

91.275

0.369
  pilA Vibrio campbellii strain DS40M4

38.298

94.631

0.362

Multiple sequence alignment