Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BUM88_RS01575 Genome accession   NZ_CP020000
Coordinates   323964..325676 (-) Length   570 a.a.
NCBI ID   WP_080026206.1    Uniprot ID   -
Organism   Acinetobacter calcoaceticus strain CA16     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 318964..330676
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BUM88_RS01545 (BUM88_01545) - 319128..319454 (+) 327 WP_003654567.1 pyrimidine/purine nucleoside phosphorylase -
  BUM88_RS01550 (BUM88_01550) rlmB 319557..320306 (+) 750 WP_005037200.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  BUM88_RS01555 (BUM88_01555) - 320342..321256 (+) 915 WP_004640840.1 DMT family transporter -
  BUM88_RS01560 (BUM88_01560) coaE 321253..321849 (-) 597 WP_004640841.1 dephospho-CoA kinase -
  BUM88_RS20340 (BUM88_01565) pilD 321851..322711 (-) 861 WP_080026205.1 prepilin peptidase Machinery gene
  BUM88_RS01570 (BUM88_01570) pilC 322711..323937 (-) 1227 WP_004640845.1 type II secretion system F family protein Machinery gene
  BUM88_RS01575 (BUM88_01575) pilB 323964..325676 (-) 1713 WP_080026206.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BUM88_RS01580 (BUM88_01580) tpiA 325968..326762 (+) 795 WP_003654580.1 triose-phosphate isomerase -
  BUM88_RS01585 (BUM88_01585) secG 326775..327104 (+) 330 WP_005037186.1 preprotein translocase subunit SecG -
  BUM88_RS01605 (BUM88_01605) rimP 327745..328269 (+) 525 WP_005037183.1 ribosome maturation factor RimP -
  BUM88_RS01610 (BUM88_01610) nusA 328306..329790 (+) 1485 WP_003654584.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63532.88 Da        Isoelectric Point: 5.0251

>NTDB_id=219714 BUM88_RS01575 WP_080026206.1 323964..325676(-) (pilB) [Acinetobacter calcoaceticus strain CA16]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKQDTVSYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRVLPLVQRGQILYVATSNPNNIEAIDAIRFNSKLMVEQVIVEHHKLEKILAQQFTEESSFEFSDEEF
DLDVDLDTSTAPEEDEDTPQGDEAPIVKYVNKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLSFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKVPSDIPKQSLLEMGF
TEQDLIHPDFQVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFSNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=219714 BUM88_RS01575 WP_080026206.1 323964..325676(-) (pilB) [Acinetobacter calcoaceticus strain CA16]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCGGCAAAAAGAGCTAAACAGGATACAGTTTCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACCAGCCAGATT
CCTAAAGATTTGGTCGACCAGAAACTGATTTTAAAACATCGGGTCTTGCCTCTCGTACAAAGAGGACAGATTTTATATGT
TGCTACCAGTAACCCCAATAATATTGAAGCGATTGATGCTATTCGCTTCAACAGCAAGCTTATGGTTGAGCAGGTTATTG
TCGAGCATCATAAACTTGAGAAAATTCTGGCGCAGCAATTTACTGAAGAAAGCAGTTTCGAGTTTAGTGATGAAGAGTTT
GATCTTGATGTAGATCTTGATACCTCAACCGCTCCAGAAGAGGATGAGGATACTCCGCAAGGCGATGAAGCGCCTATTGT
TAAATATGTTAACAAGTTATTAATAGATGCGATTCGTATGGGGGCTTCTGATTTACATTTCGAACCCTATGAGAAATCGT
ATCGGGTCCGTTATCGGGTCGATGGCGTTTTGCGTCAGATTGCAAACCCACCTTTGCAACTAGCTAACCGTTTGGCTTCA
CGCTTGAAAGTTATGTCTCAAATGGACATTTCCGAAAAGCGTGTTCCTCAAGATGGTCGTATCAAACTCAAGCTATCGAA
ATCTAAAGCAATTGATTTTCGTGTGAACTCTCTTCCGACCTTATTTGGTGAAAAGCTAGTTCTACGTATTCTCGATCCAT
CTAGTGCCATGCTTGGGATTGATGCTCTAGGATATGAAGAAGAGCAAAAAGCACTCTTTATGGAAGCATTAGATAAGCCA
CAAGGTATGCTTTTAATTACTGGTCCTACAGGTTCTGGTAAAACAGTATCCTTATATACGGGCCTCAACATTTTAAACAC
TGAAAGTTCCAATATTTCTACTGCCGAAGATCCAGTCGAAATTAACCTTGAAGGTATTAATCAGGTTAACGTGAACCCTA
AGGTAGGTCTTAGTTTTTCGGCCGCGCTTAAATCATTTTTACGTCAGGATCCTGACATTATTATGGTCGGTGAGATTCGT
GATTTAGAAACAGCTGAAATCGCGATTAAGGCTGCTCAGACGGGCCACATGGTAATGTCCACACTACATACCAACAGTGC
CCCTGAAACACTTACTCGTCTACGCAATATGGGAGTTCCATCTTTTAATATTGCGACATCGGTCAATTTGGTTATTGCTC
AACGTTTGGCACGTCGATTATGCCCTCAATGTAAGGTGCCTAGTGACATTCCTAAACAAAGCCTATTAGAAATGGGTTTT
ACCGAACAAGATCTAATACATCCCGATTTTCAAGTTTTTCAACCAGTCGGTTGTCCTGAATGTCGTGAGGGTTACAAAGG
TCGAGTAGGTATTTATGAAGTAATGAAAGTCACACCTGAGATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAATTGGGGTTTAGTAATCTGCGTCGCTCAGGTTTAAAGAAAGTCATGCAAGGTGTTACTTCT
TTACAAGAAGTCAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

93.86

100

0.939
  pilB Acinetobacter baylyi ADP1

78.947

100

0.789
  pilB Legionella pneumophila strain ERS1305867

52.014

100

0.521
  pilB Vibrio parahaemolyticus RIMD 2210633

52.075

92.982

0.484
  pilF Neisseria gonorrhoeae MS11

48.754

98.596

0.481
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

50.096

91.053

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.668

100

0.384

Multiple sequence alignment