Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   B2D37_RS14905 Genome accession   NZ_CP019947
Coordinates   3277796..3279907 (-) Length   703 a.a.
NCBI ID   WP_079204994.1    Uniprot ID   -
Organism   Pseudomonas sp. CC6-YY-74     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3272796..3284907
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B2D37_RS14890 - 3274532..3276106 (-) 1575 WP_079203419.1 AAA family ATPase -
  B2D37_RS14895 aroB 3276117..3277220 (-) 1104 WP_079203420.1 3-dehydroquinate synthase -
  B2D37_RS23755 aroK 3277272..3277790 (-) 519 WP_079203421.1 shikimate kinase AroK -
  B2D37_RS14905 pilQ 3277796..3279907 (-) 2112 WP_079204994.1 type IV pilus secretin PilQ Machinery gene
  B2D37_RS14910 pilP 3279962..3280489 (-) 528 WP_079203422.1 type 4a pilus biogenesis lipoprotein PilP -
  B2D37_RS14915 pilO 3280486..3281109 (-) 624 WP_079203423.1 type 4a pilus biogenesis protein PilO -
  B2D37_RS14920 pilN 3281106..3281681 (-) 576 WP_079203424.1 type 4a pilus biogenesis protein PilN -
  B2D37_RS14925 pilM 3281681..3282745 (-) 1065 WP_079203425.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 703 a.a.        Molecular weight: 75555.74 Da        Isoelectric Point: 4.8894

>NTDB_id=219089 B2D37_RS14905 WP_079204994.1 3277796..3279907(-) (pilQ) [Pseudomonas sp. CC6-YY-74]
MNSPLSHLGISLLALLLSPALLAADLQALDVSALPGDRVELKLTFDEPVLAPRGYTIEQPARIALDFPGVSSKLGTKNRE
LGVGNARSVTVVEAKDRTRLIINLTSLAPYNTRADGNNLYVVVGEGASGSASVRPAAAAAVVSTAAPVKSYAAHGKAISN
IDFQRGEQGEGNVVITLSDASVSPDIQEQGGKIRLDFAKTQLPEALRVRLDVKDFATPVQFVSATGSADKASIVIEPTGF
YDYLAYQTDNKLTLSIKPLTQDEVETRKSERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVRGNITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKASDMAKLFQSVTSAEGS
VDERGSITVDDRTNSIIAYQTQGRLDELRRIVAQLDIPVRQVMVEARIVEANVDFDKSLGVRWGGNFWSGDKWNAWGKDG
NLGVEDGDVGQVGRFPGIRETPLNTPFVDLGAAGATTGIGIGFVTNNVLLDLQLSAMEKTGNGEVISQPKVVTSDKETAK
ILKGSEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFSNAAASGGVPAINKNEVNAKVLVADG
ETIVIGGVFTNTQSKSVDKVPFLGDIPYLGVLFRRDLVKDSKSELLIFLTPRIMNTQAVAVSR

Nucleotide


Download         Length: 2112 bp        

>NTDB_id=219089 B2D37_RS14905 WP_079204994.1 3277796..3279907(-) (pilQ) [Pseudomonas sp. CC6-YY-74]
ATGAATAGCCCCTTATCGCATCTAGGTATCTCTCTATTAGCGTTGTTGCTATCGCCGGCCTTGTTGGCCGCTGACCTGCA
GGCGCTTGATGTTTCTGCGTTGCCAGGTGATCGCGTCGAATTGAAGTTGACGTTCGATGAGCCGGTGCTTGCTCCGCGCG
GCTATACGATAGAGCAGCCGGCGCGGATTGCCCTGGATTTCCCGGGTGTTTCCAGCAAATTGGGTACGAAGAACCGAGAG
CTGGGCGTGGGTAACGCGCGCAGTGTCACCGTGGTCGAAGCCAAGGACCGGACCCGGTTGATCATCAATCTGACCAGCTT
GGCTCCCTACAATACGCGGGCCGATGGCAACAACCTTTATGTTGTGGTTGGTGAAGGGGCGTCCGGCTCAGCGTCAGTGC
GGCCGGCGGCTGCAGCGGCAGTTGTCAGTACCGCTGCGCCGGTAAAGAGCTATGCCGCACATGGCAAGGCTATTAGCAAT
ATTGATTTCCAGCGGGGCGAGCAAGGCGAGGGTAATGTTGTGATTACCCTGTCGGATGCTTCGGTAAGCCCTGATATTCA
AGAGCAGGGTGGCAAGATTCGCCTGGACTTCGCCAAGACTCAATTGCCTGAGGCGCTACGCGTTCGCCTGGACGTCAAGG
ATTTCGCTACGCCAGTACAGTTCGTCAGCGCCACGGGCTCGGCTGACAAAGCGAGTATCGTGATCGAGCCGACAGGTTTC
TATGATTATCTGGCATATCAAACCGACAATAAGCTCACGCTCAGCATCAAGCCGTTGACCCAGGATGAAGTTGAAACGCG
TAAAAGCGAGCGGTTTGCTTATAGTGGCGAGAAGTTGTCCTTAAATTTCCAGGACATCGATGTTCGCTCAGTACTGCAGT
TGATCGCGGACTTCACCGATCTGAACCTGGTGGCCAGCGATACAGTACGGGGCAACATTACCTTGCGCTTGCAGAATGTG
CCTTGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAAGGCCTGGACAAGCGCAAGGTGGGCAATGTGCTGTTGGTGGC
TCCGGCGGATGAGATTGCCGCGCGCGAGCGTCAGGAACTCGAGTCGCAGAAACAGATTGCAGAGTTGGCGCCACTGCGGC
GCGAGCTGATTCAAGTGAACTATGCCAAGGCCAGCGACATGGCCAAGTTGTTCCAGTCGGTCACCAGCGCAGAGGGCTCT
GTAGATGAACGCGGCTCGATAACGGTCGACGACAGAACCAACAGCATCATTGCTTATCAGACTCAGGGTCGTCTGGATGA
GTTGCGCCGGATCGTCGCTCAACTCGATATTCCGGTGCGTCAGGTGATGGTGGAGGCGCGGATCGTCGAGGCCAATGTCG
ACTTCGACAAGTCACTGGGTGTTCGTTGGGGAGGTAATTTCTGGTCTGGTGACAAATGGAATGCTTGGGGCAAGGATGGA
AACCTCGGGGTCGAGGATGGTGACGTGGGGCAAGTTGGGCGTTTTCCCGGTATTCGCGAAACGCCATTAAATACCCCTTT
TGTTGACTTAGGTGCTGCCGGCGCTACGACGGGTATTGGTATAGGTTTTGTTACCAACAACGTTCTTCTTGATCTCCAGT
TAAGCGCCATGGAGAAAACCGGCAACGGTGAGGTTATTTCTCAGCCAAAAGTTGTGACCTCGGATAAAGAGACGGCAAAA
ATATTGAAGGGTTCTGAGGTTCCCTATCAGGAAGCGAGCTCAAGTGGTGCTACGAGCACCTCTTTCAAGGAAGCCGCGCT
TTCGTTGGAAGTGACGCCGCAGATTACGCCGGATAATCGCATCATCATGGAGGTCAAGGTCACCAAGGATGCACCGGATT
TCTCTAATGCGGCTGCGAGTGGTGGGGTCCCTGCGATTAATAAGAATGAGGTGAATGCCAAAGTTCTGGTTGCTGACGGT
GAAACTATTGTCATAGGTGGCGTGTTTACCAACACCCAGAGCAAGTCGGTAGACAAAGTTCCGTTCCTTGGCGATATTCC
GTACTTGGGTGTCCTGTTCAGGCGCGACCTGGTCAAAGATAGCAAATCGGAACTGTTGATTTTCCTCACTCCGCGCATCA
TGAACACTCAGGCTGTAGCGGTATCTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(536-694)

Secretin and TonB N terminus short domain (STN)

(302-348)

AMIN domain (AMIN)

(39-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

76.848

100

0.784
  comQ Acinetobacter baylyi ADP1

41.963

100

0.42
  pilQ Acinetobacter baumannii strain A118

41.418

100

0.415
  pilQ Acinetobacter baumannii D1279779

41.418

100

0.415
  pilQ Legionella pneumophila strain ERS1305867

38.304

97.297

0.373
  pilQ Legionella pneumophila strain Lp02

38.158

97.297

0.371

Multiple sequence alignment