Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   B2D37_RS14925 Genome accession   NZ_CP019947
Coordinates   3281681..3282745 (-) Length   354 a.a.
NCBI ID   WP_079203425.1    Uniprot ID   -
Organism   Pseudomonas sp. CC6-YY-74     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3276681..3287745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B2D37_RS23755 aroK 3277272..3277790 (-) 519 WP_079203421.1 shikimate kinase AroK -
  B2D37_RS14905 pilQ 3277796..3279907 (-) 2112 WP_079204994.1 type IV pilus secretin PilQ Machinery gene
  B2D37_RS14910 pilP 3279962..3280489 (-) 528 WP_079203422.1 type 4a pilus biogenesis lipoprotein PilP -
  B2D37_RS14915 pilO 3280486..3281109 (-) 624 WP_079203423.1 type 4a pilus biogenesis protein PilO -
  B2D37_RS14920 pilN 3281106..3281681 (-) 576 WP_079203424.1 type 4a pilus biogenesis protein PilN -
  B2D37_RS14925 pilM 3281681..3282745 (-) 1065 WP_079203425.1 pilus assembly protein PilM Machinery gene
  B2D37_RS14930 - 3282927..3285356 (+) 2430 WP_079203426.1 penicillin-binding protein 1A -
  B2D37_RS14935 - 3285638..3286906 (-) 1269 WP_079203427.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37874.20 Da        Isoelectric Point: 4.4526

>NTDB_id=219090 B2D37_RS14925 WP_079203425.1 3281681..3282745(-) (pilM) [Pseudomonas sp. CC6-YY-74]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALARVLAKAKTGVKSVAV
AVAGSAVITKTIEMEAGLSDDDLENQLKVEADQYIPYPLEEVAIDFEVQGSSPRNPDRVEVLLAACRKENVEVREAALAL
AGLVAKVVDVEAYALERAYGLLTEQLGSGHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALSSRVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=219090 B2D37_RS14925 WP_079203425.1 3281681..3282745(-) (pilM) [Pseudomonas sp. CC6-YY-74]
GTGCTAGGGCTCTTCAATAAGAAAGCGAATACGCTGCTGGGGATCGATATCAGTTCGACCTCCGTCAAGCTCCTCGAATT
GAGTCGCTCAGGAAGCCGCTACAAGGTAGAGGCTTACGCTGTCGAGCCGCTCCCACCAAATGCGGTGGTCGAGAAAAATA
TTGCCGAACTTGAGGGGGTCGGGCAGGCGCTTGCGCGTGTGCTGGCCAAAGCCAAGACCGGTGTGAAATCCGTAGCGGTC
GCTGTAGCTGGCTCCGCGGTGATTACCAAGACCATCGAGATGGAAGCCGGGCTTTCTGATGATGATCTCGAGAATCAACT
GAAGGTCGAGGCCGATCAGTACATTCCATATCCGCTTGAAGAAGTGGCTATTGATTTCGAGGTGCAAGGCTCTTCGCCGC
GGAATCCTGATCGGGTCGAGGTGCTGCTGGCAGCCTGCCGCAAGGAGAACGTAGAGGTTCGCGAGGCCGCCCTGGCCTTG
GCCGGGTTGGTCGCCAAAGTGGTCGATGTCGAAGCCTACGCCCTGGAGCGTGCTTATGGCTTGCTCACGGAGCAGCTTGG
TAGCGGGCATGACGAGCTGACGGTGGCCGTGGTCGATATTGGTGCAACCATGACCACGCTCAGCGTGTTGCACAATGGCC
GCACTATTTATACGCGTGAGCAGCTATTTGGCGGCAAGCAGCTCACCGAGGAAATCCAGCGGCGTTACGGTCTGTCCGTG
GAGGAAGCCGGTCTTGCGAAAAAACAGGGCGGCCTTCCCGATGACTACGACAGCGAAGTATTACAGCCGTTCAAGGACGC
GGTCGTACAGCAAGTTTCGCGCTCCCTGCAATTCTTCTTTGCTGCAGGTCAGTTCAATGATGTCGATTACATTCTTCTGG
CTGGCGGCACGGCTTCCATTCCCGACCTCGATCGTCTGATTCAGCAAAAGATCGGCACGCAAACCCTGGTTGCCAATCCT
TTCGCGGACATGGCACTGAGCAGCAGGGTCAATGCTGGAGCGTTGGCCAGTGATGCCCCAGCATTGATGATTGCCTGTGG
TCTGGCGATGAGGAGTTTCGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

57.062

100

0.571
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  comM Acinetobacter baylyi ADP1

52.825

100

0.528
  pilM Legionella pneumophila strain ERS1305867

46.197

100

0.463

Multiple sequence alignment