Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   RS_RS14135 Genome accession   NC_003295
Coordinates   3040292..3041557 (+) Length   421 a.a.
NCBI ID   WP_011002732.1    Uniprot ID   A0A223GQI2
Organism   Ralstonia pseudosolanacearum GMI1000     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3035292..3046557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS_RS14115 (RS00282) ispB 3035739..3036707 (+) 969 WP_011002729.1 octaprenyl diphosphate synthase -
  RS_RS14125 (RS00281) - 3036995..3038272 (+) 1278 WP_011002730.1 HlyC/CorC family transporter -
  RS_RS14130 (RS00280) pilB 3038520..3040247 (+) 1728 WP_011002731.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RS_RS14135 (RS00279) pilC 3040292..3041557 (+) 1266 WP_011002732.1 type II secretion system F family protein Machinery gene
  RS_RS14140 (RS00278) - 3041560..3042441 (+) 882 WP_029240316.1 prepilin peptidase -
  RS_RS14145 (RS00277) coaE 3042454..3043068 (+) 615 WP_011002734.1 dephospho-CoA kinase -
  RS_RS14150 (RS00276) zapD 3043186..3043944 (+) 759 WP_011002735.1 cell division protein ZapD -
  RS_RS14155 (RS00275) yacG 3043955..3044170 (+) 216 WP_011002736.1 DNA gyrase inhibitor YacG -
  RS_RS14160 (RS00274) - 3044163..3044624 (-) 462 WP_011002737.1 NUDIX domain-containing protein -
  RS_RS14165 (RS00273) - 3044602..3045486 (-) 885 WP_011002738.1 ATP-binding protein -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 46569.86 Da        Isoelectric Point: 10.3648

>NTDB_id=21868 RS_RS14135 WP_011002732.1 3040292..3041557(+) (pilC) [Ralstonia pseudosolanacearum GMI1000]
MATRAPTANRSAAASARKTAATKAPTQYIFEWEGKDRKGKIFKGEMRAESITEVNAVLRKQGLSITKSKRRRAARGKKIV
PKDIAYFTRQLSTMLKAGVPLLQSVDIIARGHANPNFTQLLTEIRVDIESGSSMAQAFRRHPQHFDSLYCNLIDAGEQGG
ILDALLERLSMYMEKSIALKAQIKSAMIYPISVLTVAFLVTVVLMIFVVPAFKNVFASFGATLPAPTLVVMGISDFFVKW
WYIIIFTPIVGIFLYVRAFKRSEKVQRNTHKAILKMPIFGSIIRKATIARWTRTLATMFSAGTPLVESMESVAGAAGNWV
YYDATMEIQQAVRIGTSLTNAMQATHVFDNMVLQMTQIGEESGALDNMLLKVAEFYEREVDDAVANISALIEPLIIVILG
VLIGGMVVAMYLPIFKLGQVV

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=21868 RS_RS14135 WP_011002732.1 3040292..3041557(+) (pilC) [Ralstonia pseudosolanacearum GMI1000]
ATGGCCACACGAGCACCGACCGCGAACCGGTCCGCCGCCGCATCGGCACGCAAGACCGCCGCCACGAAGGCGCCCACGCA
GTACATCTTCGAGTGGGAAGGCAAGGACCGCAAAGGCAAGATCTTCAAGGGCGAGATGCGGGCGGAGAGCATCACCGAAG
TCAATGCGGTGCTGCGCAAGCAAGGGCTGTCGATCACCAAGTCCAAGCGTCGACGCGCCGCGCGCGGCAAGAAGATCGTA
CCCAAGGACATCGCCTACTTCACGCGCCAGCTGTCGACCATGCTGAAAGCGGGCGTGCCGCTGCTGCAATCGGTCGACAT
CATCGCGCGCGGGCACGCCAACCCGAACTTCACCCAGCTGCTGACGGAAATCCGTGTCGACATCGAGTCGGGCAGCAGCA
TGGCGCAGGCCTTCCGCCGCCATCCGCAGCACTTCGATTCGCTGTACTGCAACCTGATCGACGCCGGTGAGCAGGGCGGT
ATTCTCGACGCGCTGCTCGAGCGGCTGTCGATGTACATGGAAAAGTCGATCGCGCTGAAGGCGCAGATCAAGTCGGCCAT
GATCTATCCGATCTCGGTGCTGACGGTGGCTTTCCTGGTCACGGTGGTGCTGATGATCTTCGTGGTGCCGGCGTTCAAGA
ACGTGTTCGCCAGCTTCGGCGCCACGCTGCCGGCCCCGACGCTGGTGGTGATGGGCATTTCGGACTTCTTCGTGAAGTGG
TGGTACATCATCATCTTCACGCCCATTGTCGGCATCTTCCTGTATGTGCGGGCTTTCAAGCGCTCGGAGAAGGTGCAGCG
CAACACGCACAAGGCCATCCTGAAGATGCCGATCTTCGGCAGCATCATCCGCAAGGCCACCATCGCGCGCTGGACGCGTA
CGCTGGCAACCATGTTCTCGGCCGGGACACCGCTGGTGGAGTCGATGGAATCCGTCGCGGGCGCCGCCGGCAACTGGGTC
TACTATGACGCCACCATGGAGATCCAGCAGGCGGTGCGCATCGGTACCAGCCTCACCAACGCCATGCAGGCGACCCATGT
GTTCGACAACATGGTGCTGCAGATGACGCAGATCGGCGAGGAATCCGGCGCGCTGGACAACATGCTGCTGAAGGTGGCCG
AATTCTACGAGCGCGAGGTCGATGACGCGGTGGCCAACATCTCCGCCCTGATCGAGCCGCTCATCATCGTGATCCTGGGC
GTGCTGATCGGGGGCATGGTGGTTGCCATGTACCTGCCGATCTTCAAGCTGGGCCAAGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A223GQI2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

50.248

95.962

0.482
  pilG Neisseria gonorrhoeae MS11

50.125

94.774

0.475
  pilG Neisseria meningitidis 44/76-A

49.875

94.774

0.473
  pilC Acinetobacter baumannii D1279779

47.912

96.675

0.463
  pilC Acinetobacter baylyi ADP1

46.683

96.675

0.451
  pilC Legionella pneumophila strain ERS1305867

45.813

96.437

0.442
  pilC Vibrio cholerae strain A1552

40

95.012

0.38
  pilC Vibrio campbellii strain DS40M4

39.506

96.2

0.38

Multiple sequence alignment