Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   B0D95_RS10540 Genome accession   NZ_CP019799
Coordinates   2402927..2404654 (+) Length   575 a.a.
NCBI ID   WP_078043866.1    Uniprot ID   A0A1U9NAE6
Organism   Cellvibrio sp. PSBB023     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2397927..2409654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B0D95_RS10525 (B0D95_10500) - 2398244..2400778 (-) 2535 WP_078043863.1 DUF1631 domain-containing protein -
  B0D95_RS10530 (B0D95_10505) nadC 2400996..2401850 (+) 855 WP_078043864.1 carboxylating nicotinate-nucleotide diphosphorylase -
  B0D95_RS10535 (B0D95_10510) - 2402128..2402472 (-) 345 WP_371453658.1 pilin -
  B0D95_RS21120 - 2402493..2402601 (-) 109 Protein_2075 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  B0D95_RS10540 (B0D95_10515) pilB 2402927..2404654 (+) 1728 WP_078043866.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B0D95_RS10545 (B0D95_10520) pilC 2404658..2405938 (+) 1281 WP_078043867.1 type II secretion system F family protein Machinery gene
  B0D95_RS10550 (B0D95_10525) pilD 2405983..2406837 (+) 855 WP_078045710.1 A24 family peptidase Machinery gene
  B0D95_RS10555 (B0D95_10530) coaE 2406898..2407509 (+) 612 WP_078043868.1 dephospho-CoA kinase -
  B0D95_RS10560 (B0D95_10535) yacG 2407518..2407721 (+) 204 WP_078043869.1 DNA gyrase inhibitor YacG -
  B0D95_RS10565 (B0D95_10540) - 2407750..2408703 (-) 954 WP_210403613.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62732.92 Da        Isoelectric Point: 5.5831

>NTDB_id=218423 B0D95_RS10540 WP_078043866.1 2402927..2404654(+) (pilB) [Cellvibrio sp. PSBB023]
MNSPTPVSLSGLARRLVADKILEAELAAKALAQAKKDKIPFAQHLVNHSIIDARTLALVASEEFGSPVFDLNALNKDGIP
QKLVDDKLIRKHHTLPIARRGNRLFLAVTDPTDLHALDEIKFNTGINTEAVLVEADKLQIAIDKYLNSQEESLGDALGGL
DDDIDDLDVQSVSDAVTNEDASTEADEAPIVKYINKVLLDAIKGGASDIHFEPYEKSYRIRFRTDGVLNEVSRPPVNLAF
RMAARLKVMSQMDISERRLPQDGRIKLKVSKTRAIDFRVNTLPTLFGEKIVLRILDPSSAKLGIDALGYEDDQKKLYMDA
LAQSQGMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLEGINQVQMNTRVGLTFAEALRSFLRQDPDIIMV
GEIRDLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPAFNVATSVSLIIAQRLARRLCSACKKPATDIPADIL
TQEGFDEIGIPRTEFQLFHPGGCEKCNGGYKGRVGVYEVVRITPLIANLIMEGGNSLQIARAAKEAGFNNLRVSALRKVA
MGLTSLEEANRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=218423 B0D95_RS10540 WP_078043866.1 2402927..2404654(+) (pilB) [Cellvibrio sp. PSBB023]
ATGAACAGTCCAACCCCGGTGTCATTAAGCGGACTAGCCCGCAGACTAGTCGCAGACAAAATATTAGAGGCAGAGCTGGC
TGCTAAAGCTCTTGCCCAAGCAAAAAAAGACAAAATCCCATTTGCCCAGCATCTGGTTAATCACTCTATAATTGATGCGC
GCACTTTGGCATTAGTGGCATCTGAAGAGTTTGGCTCACCCGTATTTGATTTAAATGCGCTCAATAAAGATGGAATCCCT
CAAAAACTGGTTGACGACAAACTGATTAGGAAACATCACACCCTACCTATCGCAAGACGCGGCAATCGCTTATTTCTTGC
TGTTACCGACCCAACCGATCTGCATGCCCTAGATGAAATCAAGTTCAACACAGGGATTAATACAGAAGCCGTCTTAGTTG
AAGCGGACAAGTTGCAAATCGCTATTGATAAATACTTAAATTCGCAAGAAGAGTCTCTGGGCGATGCATTAGGCGGCCTT
GATGACGACATTGATGATTTGGATGTGCAGTCAGTTAGCGACGCAGTAACCAATGAAGATGCAAGCACAGAGGCTGACGA
GGCACCAATCGTAAAGTACATCAATAAAGTGCTATTGGATGCAATTAAAGGTGGGGCGTCGGATATCCACTTTGAACCCT
ACGAAAAAAGCTATCGCATACGCTTCCGTACCGACGGCGTTCTGAATGAAGTGTCCCGTCCGCCGGTAAACCTTGCATTT
CGTATGGCGGCGCGCTTAAAAGTCATGTCGCAAATGGATATTTCCGAGCGCCGTTTGCCGCAGGATGGACGTATCAAGCT
CAAGGTATCCAAAACGCGCGCTATCGACTTCCGGGTAAATACCCTGCCTACGCTTTTTGGTGAAAAGATCGTTCTGCGTA
TTCTTGACCCTAGTTCTGCCAAACTAGGGATCGATGCACTGGGTTATGAGGATGACCAGAAGAAACTCTACATGGACGCC
CTCGCACAATCGCAAGGGATGATTCTGGTAACCGGCCCAACAGGTAGCGGTAAAACAGTCTCGCTCTATACCGGCCTGAA
TATCCTGAATACATCAGAAGTGAATATCTCTACAGCGGAAGACCCTGTTGAAATTAACCTGGAAGGAATTAATCAGGTAC
AAATGAACACCCGCGTCGGGTTAACCTTTGCAGAAGCACTGCGCTCTTTCCTGCGTCAAGACCCGGATATCATTATGGTG
GGTGAGATTCGTGACTTGGAAACAGCGGAAATTGCCATTAAAGCAGCTCAAACTGGCCACTTGGTTCTTTCCACACTTCA
CACCAACTCTGCACCAGAAACACTTACCCGCCTATTAAACATGGGCGTTCCTGCGTTTAACGTAGCAACCAGCGTAAGCT
TGATTATTGCCCAACGGCTTGCACGACGCTTGTGCAGTGCTTGCAAAAAACCAGCAACGGATATCCCGGCAGATATACTC
ACTCAAGAAGGGTTTGACGAAATCGGAATCCCTCGCACAGAGTTCCAGTTATTCCACCCGGGTGGTTGTGAAAAATGTAA
CGGGGGTTATAAAGGACGCGTAGGTGTATATGAAGTGGTTCGCATCACACCATTAATTGCCAACCTTATAATGGAAGGCG
GCAACTCGTTACAGATTGCTCGCGCCGCGAAAGAAGCGGGCTTTAATAATTTACGGGTATCTGCATTGCGTAAAGTAGCA
ATGGGATTAACCAGCCTCGAAGAAGCCAACCGAGTAACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-151)

Type II/IV secretion system protein (T2SSE)

(196-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1U9NAE6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.189

99.478

0.609
  pilB Acinetobacter baylyi ADP1

58.362

99.826

0.583
  pilB Legionella pneumophila strain ERS1305867

54.276

99.652

0.541
  pilB Vibrio cholerae strain A1552

54.291

93.217

0.506
  pilB Vibrio campbellii strain DS40M4

50.877

99.13

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

51.188

95.13

0.487
  pilF Neisseria gonorrhoeae MS11

48.33

98.957

0.478
  pilB/pilB1 Synechocystis sp. PCC 6803

41.506

90.087

0.374

Multiple sequence alignment