Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   B1F84_RS13400 Genome accession   NZ_CP019770
Coordinates   2868543..2870219 (+) Length   558 a.a.
NCBI ID   WP_131691712.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. DL-6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2863543..2875219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B1F84_RS13380 (B1F84_13380) pilA2 2864882..2865319 (+) 438 WP_131691708.1 pilin Machinery gene
  B1F84_RS13385 (B1F84_13385) pilC 2865386..2866615 (+) 1230 WP_131691709.1 type II secretion system F family protein Machinery gene
  B1F84_RS13390 (B1F84_13390) pilD 2866855..2867766 (+) 912 WP_131691710.1 A24 family peptidase Machinery gene
  B1F84_RS13395 (B1F84_13395) coaE 2867769..2868398 (+) 630 WP_131691711.1 dephospho-CoA kinase -
  B1F84_RS13400 (B1F84_13400) pilB 2868543..2870219 (+) 1677 WP_131691712.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B1F84_RS13405 (B1F84_13405) yacG 2870285..2870515 (+) 231 WP_008466423.1 DNA gyrase inhibitor YacG -
  B1F84_RS13410 (B1F84_13410) - 2870809..2871273 (-) 465 WP_131691713.1 Spy/CpxP family protein refolding chaperone -
  B1F84_RS13415 (B1F84_13415) - 2871434..2872120 (+) 687 WP_076919373.1 response regulator -
  B1F84_RS13420 (B1F84_13420) - 2872098..2873525 (+) 1428 WP_131691714.1 ATP-binding protein -
  B1F84_RS13425 (B1F84_13425) - 2873515..2874459 (+) 945 WP_131691715.1 alpha/beta fold hydrolase -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62336.76 Da        Isoelectric Point: 5.9661

>NTDB_id=218279 B1F84_RS13400 WP_131691712.1 2868543..2870219(+) (pilB) [Pseudoalteromonas sp. DL-6]
MNINSPLLRKFITLGRVDTDTVKAKQQEFASTAELISKCGKIDSQDLAQQCIDLFRVPFFDLKDFNVAQISAELVKEKLI
RKHHILPLVQKGRKVYIAASDPTDYGAFENFEFSTGLSCEVVVVDYLQLDHKIEQLLDATGSLNLSEDEFKEFAELDSEP
QRDNTPKDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNSLATKLSARIKVMSRLDI
AEKRKPQDGRIKLKITERKSIDFRVSTMPTLWGEKVVMRILDSSSAMLGIDVLGYEPEQKKLYLDALEQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGVNQVQINIKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCSKCKVPEPLPREELIRQGFNEQQIQDMIL
YAPKGCESCTDGYKGRVGIYEVMKVTPEIAQIIMRGGNSLEIAEVSLKAGFNNIRLSGLRKAAEGVTSLLEINRVTSF

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=218279 B1F84_RS13400 WP_131691712.1 2868543..2870219(+) (pilB) [Pseudoalteromonas sp. DL-6]
ATGAATATTAACTCACCGTTATTAAGAAAATTTATTACCCTCGGCCGGGTTGATACCGATACCGTAAAAGCAAAACAACA
AGAGTTTGCGTCAACTGCTGAGCTTATTTCTAAATGTGGCAAAATAGACAGCCAAGATTTAGCGCAACAATGTATTGATT
TGTTTAGAGTGCCTTTTTTTGATTTAAAAGATTTTAATGTTGCACAAATATCTGCCGAGCTGGTTAAAGAAAAGCTGATC
CGCAAGCACCATATTTTACCTTTGGTACAAAAAGGCAGAAAAGTATACATTGCTGCCTCTGATCCTACCGATTACGGCGC
TTTTGAAAACTTTGAATTTAGCACCGGTTTATCATGCGAAGTGGTGGTGGTTGACTACCTACAGCTCGACCATAAAATTG
AGCAACTACTGGATGCCACAGGTAGCTTAAACTTAAGTGAAGATGAATTTAAAGAGTTTGCTGAACTAGACTCTGAGCCA
CAAAGAGATAACACCCCTAAAGATGATGACAAAGACGATGCCCCTATTATTGTCTACATTAATAAAATTTTAATGGATGC
AATAAAAAAAGGTGCTTCCGATCTTCACTTTGAACCCTATGAGCACAAATACCGTATTCGCTTTCGTATTGATGGTATTT
TACATGAGGTAGCTAGTCCACCGAATAGTTTAGCAACCAAGCTTTCGGCGCGTATAAAAGTTATGTCACGCCTTGATATC
GCTGAAAAACGTAAACCACAAGATGGCCGTATCAAGCTTAAAATTACTGAGCGTAAAAGTATTGATTTTCGTGTTAGTAC
CATGCCAACCCTATGGGGTGAAAAAGTGGTAATGCGTATTCTAGATTCATCCAGTGCTATGCTAGGCATTGATGTATTAG
GTTATGAACCCGAACAGAAAAAACTCTACCTCGATGCACTAGAACAACCACAAGGGATGATTTTAGTGACCGGGCCAACC
GGCTCGGGTAAAACCGTATCCTTGTATACGGGTTTAAATATACTTAATCAGCCGGAACGAAATATTAGTACCGCTGAAGA
TCCGGTAGAAATTAACCTCGAAGGGGTTAACCAAGTACAAATAAATATTAAAGCCGACATGACCTTTGCTAATGCTTTAC
GGGCGTTCTTACGTCAAGATCCCGATGTTGTCATGGTGGGTGAAATACGTGATTTAGAAACCGCTGAAATATCAATTAAA
GCGGCGCAAACCGGTCACTTGGTGCTATCAACGCTTCACACTAACTCCGCGCCAGAAACCATTACCCGTTTGTTAAATAT
GGGTGTACCCGCGTATAATGTAGCCAGTTCAATTAGTTTAATTATAGCCCAGCGCTTAGCGCGCCGATTGTGCTCTAAAT
GTAAAGTACCAGAGCCTTTACCCCGTGAAGAGCTTATTCGCCAAGGCTTTAATGAACAACAAATACAGGACATGATTTTA
TACGCACCGAAAGGCTGTGAAAGCTGTACCGATGGCTATAAAGGCCGTGTTGGTATTTACGAAGTCATGAAAGTTACCCC
AGAAATAGCGCAAATTATAATGCGCGGTGGTAACTCATTAGAAATTGCTGAGGTATCACTTAAAGCAGGGTTTAATAACA
TACGCTTATCAGGTCTTCGAAAAGCCGCAGAGGGCGTGACCTCACTCCTTGAGATAAACCGTGTCACTAGTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-140)

Type II/IV secretion system protein (T2SSE)

(182-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.545

98.566

0.557
  pilB Acinetobacter baylyi ADP1

58.416

90.502

0.529
  pilB Legionella pneumophila strain ERS1305867

53.974

96.953

0.523
  pilB Vibrio cholerae strain A1552

52.593

96.774

0.509
  pilB Vibrio parahaemolyticus RIMD 2210633

51.776

95.878

0.496
  pilB Vibrio campbellii strain DS40M4

51.215

95.878

0.491
  pilF Neisseria gonorrhoeae MS11

49.143

94.086

0.462
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.321

100

0.375

Multiple sequence alignment