Detailed information NTG0217498

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ADP71_RS02550
• Genome accession: NZ_CP019644
• Coordinates: 515824..517002 (-)
• Length: 392 a.a.
• NCBI ID: WP_104931425.1
• Uniprot ID: -
• Organism: Vitreoscilla sp. C1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 510824..522002

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ADP71_RS02530 (ADP71_06320)	-	511268..513154 (+)	1887	WP_171460866.1	alpha/beta hydrolase	-
ADP71_RS02535 (ADP71_06350)	-	513507..513809 (+)	303	WP_019957387.1	hypothetical protein	-
ADP71_RS02540 (ADP71_06360)	-	513875..514543 (-)	669	WP_104931424.1	DsbA family oxidoreductase	-
ADP71_RS02545 (ADP71_06370)	-	514779..515744 (+)	966	WP_019957389.1	histone deacetylase family protein	-
ADP71_RS02550 (ADP71_06380)	pilU	515824..517002 (-)	1179	WP_104931425.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ADP71_RS02555 (ADP71_06390)	-	517323..518123 (-)	801	WP_019957391.1	3-hydroxybutyrate dehydrogenase	-
ADP71_RS02560 (ADP71_06400)	-	518221..518850 (-)	630	WP_019957392.1	3-oxoacid CoA-transferase subunit B	-
ADP71_RS02565 (ADP71_06410)	-	518864..519562 (-)	699	WP_171460867.1	CoA transferase subunit A	-
ADP71_RS13105 (ADP71_06430)	-	519759..519887 (-)	129	WP_019957394.1	hypothetical protein	-
ADP71_RS02570 (ADP71_06440)	-	519987..520856 (-)	870	WP_019957395.1	NAD(P)H-hydrate dehydratase	-

Sequence

Protein

• Length: 392 a.a.
• Molecular weight: 43587.27 Da
• Isoelectric Point: 5.6159

>NTDB_id=217498 ADP71_RS02550 WP_104931425.1 515824..517002(-) (pilU) [Vitreoscilla sp. C1]
MDRKQAYQYVCELLKLMLSKKSSDLFITVNYPPAMKIDGKMTKMGPQNLTATHTLALVQSIMTENQIKEFETTKECNFAI
SPNGIGRFRVNAFVQQGSAGMVLRVIPTELPTIDGLGVPEILKDVVMAKRGLCILVGGTGSGKSTTLAAMVDYRNENSHG
HIITIEDPVEFVHPHKNCVVMQREVGIDTDNWEIALKNTLRQAPDVILMGEIRDAETMEHAMAFAETGHLCLATLHANSA
NQAIDRIINFFPEERHQQLLMDLSLNLRGMVSQRLIPRKGGKGRAAAIEIMLNSPLISELIFKGETGEIKEIMKKSRELG
MQTFDQALFDLYMQDKISYEEALRNADSVNDLRLTIKLHGDQKPIDGVDENHDPLAALNLMNDADLAEALNS

Nucleotide

• Length: 1179 bp

>NTDB_id=217498 ADP71_RS02550 WP_104931425.1 515824..517002(-) (pilU) [Vitreoscilla sp. C1]
ATGGATCGCAAGCAAGCCTATCAATATGTTTGTGAATTGCTCAAACTCATGTTATCCAAAAAAAGCAGTGACTTATTCAT
TACGGTGAACTACCCTCCTGCTATGAAAATTGATGGCAAAATGACCAAAATGGGTCCGCAAAATTTAACAGCGACGCATA
CTTTGGCATTGGTTCAATCCATCATGACGGAAAATCAAATCAAGGAATTTGAAACCACCAAAGAATGTAATTTTGCCATT
TCTCCCAATGGCATTGGTCGTTTTCGTGTCAATGCTTTTGTACAACAAGGTTCGGCAGGGATGGTATTGCGTGTGATTCC
AACCGAATTGCCGACCATTGATGGTTTGGGTGTGCCTGAAATTTTAAAAGATGTGGTAATGGCCAAACGTGGTTTGTGTA
TTTTGGTGGGCGGCACAGGCTCGGGCAAATCCACCACGCTCGCGGCGATGGTAGACTATCGCAATGAAAACTCACACGGT
CACATCATCACCATCGAAGACCCTGTGGAATTTGTACATCCACACAAAAACTGCGTGGTGATGCAGCGTGAAGTGGGCAT
TGATACCGACAATTGGGAAATTGCTTTGAAAAACACCTTGCGCCAAGCGCCTGATGTGATTTTGATGGGTGAGATTCGCG
ATGCGGAAACGATGGAACACGCGATGGCGTTTGCCGAAACGGGTCACTTGTGTTTGGCCACGTTGCATGCCAACAGCGCC
AACCAAGCGATTGACCGCATCATTAACTTCTTCCCAGAAGAACGCCACCAACAATTGCTGATGGACTTGTCTTTGAATTT
GCGTGGCATGGTTTCACAACGTTTGATTCCACGCAAAGGCGGTAAAGGACGTGCAGCTGCAATTGAAATCATGCTCAATA
GTCCATTGATTTCTGAATTGATTTTCAAAGGTGAAACCGGTGAAATCAAAGAAATCATGAAAAAATCGCGCGAATTGGGC
ATGCAAACCTTTGACCAAGCTTTGTTTGATTTGTATATGCAAGACAAAATTTCATACGAAGAGGCTTTGCGCAATGCCGA
CTCGGTCAACGATTTGCGCTTGACCATCAAATTGCATGGCGACCAAAAACCCATCGATGGTGTGGACGAAAACCACGACC
CATTGGCAGCATTGAATTTGATGAACGATGCTGATTTGGCAGAGGCTTTGAACTCTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (35-276)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.671	88.52	0.546
pilU	Acinetobacter baylyi ADP1	56.863	91.071	0.518
pilU	Vibrio cholerae strain A1552	54.839	86.99	0.477
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.214	85.969	0.38
pilT	Pseudomonas aeruginosa PAK	43.323	85.969	0.372
pilT	Legionella pneumophila strain Lp02	42.643	84.949	0.362
pilT	Legionella pneumophila strain ERS1305867	42.643	84.949	0.362