Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   CWI24_RS26010 Genome accession   NZ_CP025050
Coordinates   5365838..5366377 (-) Length   179 a.a.
NCBI ID   WP_100814892.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PB368     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5360838..5371377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI24_RS25990 (CWI24_25990) - 5361523..5363778 (-) 2256 WP_034027606.1 DUF1631 domain-containing protein -
  CWI24_RS25995 (CWI24_25995) nadC 5364064..5364912 (+) 849 WP_033990663.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CWI24_RS26005 (CWI24_26005) - 5365076..5365633 (-) 558 WP_224754157.1 hypothetical protein -
  CWI24_RS26010 (CWI24_26010) pilE 5365838..5366377 (-) 540 WP_100814892.1 pilin Machinery gene
  CWI24_RS26015 (CWI24_26015) pilB 5366606..5368309 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CWI24_RS26020 (CWI24_26020) pilC 5368313..5369530 (+) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene
  CWI24_RS26025 (CWI24_26025) pilD 5369533..5370405 (+) 873 WP_043085203.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  CWI24_RS26030 (CWI24_26030) coaE 5370402..5371013 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  CWI24_RS26035 (CWI24_26035) yacG 5371010..5371210 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 18029.41 Da        Isoelectric Point: 5.0333

>NTDB_id=216813 CWI24_RS26010 WP_100814892.1 5365838..5366377(-) (pilE) [Pseudomonas aeruginosa strain PB368]
MKAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRACVTEGVGLAASAKTLIGDSSATAGELAASARVWNAQAGNAGAT
SKYVTSVRIAEATGEITVTFNATNVGNIPADSTLVFTPYVQNAAGAPTQLGASYAAGVTGSIDWGCASQSNAVSSGPDRN
MPALTAGTLPARFAPSECR

Nucleotide


Download         Length: 540 bp        

>NTDB_id=216813 CWI24_RS26010 WP_100814892.1 5365838..5366377(-) (pilE) [Pseudomonas aeruginosa strain PB368]
ATGAAAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATTCTTGCTGCCGTCGCTTT
GCCGGCATATCAGGATTACACCATTCGTGCTTGCGTGACAGAGGGGGTTGGTCTGGCTGCCAGCGCCAAGACGCTTATTG
GCGATAGCTCTGCCACTGCCGGTGAGCTAGCCGCTTCGGCAAGGGTCTGGAATGCTCAAGCCGGTAACGCCGGTGCTACC
AGTAAGTATGTGACCTCTGTACGAATTGCAGAGGCGACTGGTGAAATCACTGTTACTTTCAATGCCACAAACGTGGGTAA
TATTCCGGCTGACTCTACCCTGGTATTTACTCCCTATGTGCAGAATGCTGCCGGTGCCCCGACTCAATTGGGTGCCAGTT
ATGCTGCCGGTGTGACTGGCTCTATTGACTGGGGTTGTGCATCGCAATCCAATGCGGTTTCCAGTGGTCCCGACCGCAAT
ATGCCTGCCCTGACTGCAGGTACCCTGCCGGCTCGTTTCGCTCCGAGCGAATGCCGCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-179)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

42.64

100

0.469
  pilA Ralstonia pseudosolanacearum GMI1000

43.243

100

0.447
  pilA2 Legionella pneumophila str. Paris

41.243

98.883

0.408
  pilA2 Legionella pneumophila strain ERS1305867

40.678

98.883

0.402
  comP Acinetobacter baylyi ADP1

37.778

100

0.38
  pilE Neisseria gonorrhoeae strain FA1090

35.714

100

0.363