Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PspS49_RS02100 Genome accession   NZ_CP019432
Coordinates   475382..477454 (+) Length   690 a.a.
NCBI ID   WP_150776184.1    Uniprot ID   A0A5E6WWS4
Organism   Pseudomonas sp. S49     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 470382..482454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PspS49_RS02080 (PspS49_02105) pilM 472594..473658 (+) 1065 WP_127925526.1 pilus assembly protein PilM Machinery gene
  PspS49_RS02085 (PspS49_02110) - 473658..474224 (+) 567 WP_150650018.1 PilN domain-containing protein -
  PspS49_RS02090 (PspS49_02115) pilO 474221..474844 (+) 624 WP_127925528.1 type 4a pilus biogenesis protein PilO -
  PspS49_RS02095 (PspS49_02120) - 474841..475368 (+) 528 WP_160037934.1 pilus assembly protein PilP -
  PspS49_RS02100 (PspS49_02125) pilQ 475382..477454 (+) 2073 WP_150776184.1 type IV pilus secretin PilQ Machinery gene
  PspS49_RS02105 (PspS49_02130) aroK 477459..477977 (+) 519 WP_045122915.1 shikimate kinase AroK -
  PspS49_RS02110 (PspS49_02135) aroB 478130..479230 (+) 1101 WP_160037936.1 3-dehydroquinate synthase -
  PspS49_RS02115 (PspS49_02140) - 479241..480833 (+) 1593 WP_150750143.1 AAA family ATPase -

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74758.28 Da        Isoelectric Point: 5.8240

>NTDB_id=215745 PspS49_RS02100 WP_150776184.1 475382..477454(+) (pilQ) [Pseudomonas sp. S49]
MNRIFSTLGFSLWIALMSPMVLAANLKTLDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVASQLASKNLD
LGSGNARTATVVEAKDRTRLIVSLTQLAPYTTRVAGNNLFVVVGQGAPAAAPRSAVVAPRPAAAAPAKAVVPKSRAIRGV
DFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILSFSRTQLPEKLRVRLDVKDFATPVQFVNAGVTADRTVITVEPSGTY
EYSTFQTDNKLTVSIRPMTADDLQKRNVDRQAYVGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVP
WDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAKI
DERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNASGVNGS
STTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQE
ASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQ
SKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=215745 PspS49_RS02100 WP_150776184.1 475382..477454(+) (pilQ) [Pseudomonas sp. S49]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGATGTCGCCGATGGTACTCGCGGCCAATCTGAA
GACGCTGGATGTGGCGGCGTTGCCGGGTGATCGCGTCGAGCTGAAGCTGTCGTTCGACGGCCCACCGCCACAGCCCAAGG
GCTACACCACCGAATCACCGGCACGCATCGCCCTCGATTTGCCCGGTGTCGCCAGTCAATTGGCCAGCAAGAACCTTGAT
CTGGGCAGCGGCAATGCACGCACCGCTACGGTGGTCGAGGCCAAGGATCGCACGCGGCTGATCGTGAGTCTCACGCAACT
GGCGCCTTACACCACGCGGGTCGCGGGCAACAATCTGTTCGTGGTGGTTGGGCAGGGCGCGCCGGCGGCAGCGCCGCGAT
CTGCTGTCGTTGCGCCACGCCCTGCGGCTGCAGCGCCGGCCAAGGCAGTTGTGCCGAAAAGCCGGGCTATTCGCGGCGTA
GATTTCCAGCGGGGTACGGCAGGTGAAGGCAACGTGGTCATTGACCTCTCCGACCCGACCATCGCTCCGGATATCCAGGA
GCATGACGGCAAGATCATCCTCAGCTTTTCCCGCACACAACTCCCGGAAAAGCTGCGGGTACGCCTCGACGTCAAGGATT
TCGCCACCCCGGTGCAGTTCGTCAATGCCGGAGTGACCGCTGATCGCACGGTTATTACGGTTGAACCCAGTGGTACTTAC
GAGTATTCGACTTTCCAGACCGACAACAAACTGACCGTCAGCATCCGCCCGATGACCGCCGATGATCTGCAAAAGCGTAA
CGTCGATCGTCAGGCTTACGTCGGCGAAAAGCTCTCGCTGAATTTCCAGGACATCGATGTGCGTTCAGTGCTGCAACTGA
TCGCCGATTTCACCAACCTCAACCTTGTCGCCAGCGACACGGTGCAGGGTGGCATCACCTTGCGCCTGCAGAACGTGCCG
TGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAGGGGCTGGATAAACGCAAGATCGGCAATGTGCTGCTGGTTGCTCC
GGCCGATGAAATCGCTGCCCGCGAACGTCAGGAACTGGAGTCGCAGAAACAGATCGCCGAACTGGCACCGCTGCGTCGCG
AACTGTTGCAAGTGAACTACGCGAAAGCAGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGTGCCGAGGCTAAAATC
GACGAGCGCGGTTCGATTACTGTCGATGAGCGGACCAACAACATCATTGCCTATCAGACTCAGGATCGTCTCGACGAACT
GCGGCGGATCGTGGCACAGCTGGATATACCGGTACGTCAGGTGATGATCGAGGCGCGGATCGTCGAAGCTAACGTCGATT
ACGACAAAAGCCTGGGCGTGCGCTGGGGCGGTTCGATCCAGAACAAGGGCAACTGGAACGCCTCCGGGGTCAACGGATCG
TCGACCACCATTGGTACTCCCGGCAGCACCAGTACCAACTCGCCGTTCGTCGACATGGGCACCGTCAACAATACTTCAGG
AATCGGCATCGCTTTCATCACCGACAACGTCTTGCTCGATCTTGAACTGACGGCCATGGAGAAAACCGGTAACGGAGAAA
TCGTCTCGCAGCCGAAAGTGGTCACCTCCGACAAGGAAACCGCGAAAATCCTCAAAGGCACCGAGATTCCGTATCAGGAA
GCCAGCTCCAGTGGCGCCACGTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAAGTCACGCCGCAGATCACCCCCGA
CAATCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGATTACCTGAACAAAGTGCAGGATGTACCGCCAATCA
AGAAAAACGAGGTCAACGCCAAGGTGCTGGTCAACGACGGCGAAACCATCGTGATTGGGGGCGTTTTCTCAAATACTCAA
AGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTTCGGA
GAAAAAATCCGAGCTGCTGGTATTTCTCACACCACGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Secretin and TonB N terminus short domain (STN)

(301-347)

AMIN domain (AMIN)

(37-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(375-440)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5E6WWS4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.082

100

0.759
  comQ Acinetobacter baylyi ADP1

41.474

100

0.416
  pilQ Acinetobacter baumannii strain A118

38.462

100

0.413
  pilQ Acinetobacter baumannii D1279779

38.462

100

0.413
  pilQ Legionella pneumophila strain Lp02

38.462

97.971

0.377
  pilQ Legionella pneumophila strain ERS1305867

38.427

97.681

0.375

Multiple sequence alignment