Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PspS49_RS02080 Genome accession   NZ_CP019432
Coordinates   472594..473658 (+) Length   354 a.a.
NCBI ID   WP_127925526.1    Uniprot ID   -
Organism   Pseudomonas sp. S49     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 467594..478658
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PspS49_RS02070 (PspS49_02095) - 468339..469607 (+) 1269 WP_064117089.1 malic enzyme-like NAD(P)-binding protein -
  PspS49_RS02075 (PspS49_02100) - 469930..472377 (-) 2448 WP_160037932.1 penicillin-binding protein 1A -
  PspS49_RS02080 (PspS49_02105) pilM 472594..473658 (+) 1065 WP_127925526.1 pilus assembly protein PilM Machinery gene
  PspS49_RS02085 (PspS49_02110) - 473658..474224 (+) 567 WP_150650018.1 PilN domain-containing protein -
  PspS49_RS02090 (PspS49_02115) pilO 474221..474844 (+) 624 WP_127925528.1 type 4a pilus biogenesis protein PilO -
  PspS49_RS02095 (PspS49_02120) - 474841..475368 (+) 528 WP_160037934.1 pilus assembly protein PilP -
  PspS49_RS02100 (PspS49_02125) pilQ 475382..477454 (+) 2073 WP_150776184.1 type IV pilus secretin PilQ Machinery gene
  PspS49_RS02105 (PspS49_02130) aroK 477459..477977 (+) 519 WP_045122915.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38106.74 Da        Isoelectric Point: 4.9006

>NTDB_id=215744 PspS49_RS02080 WP_127925526.1 472594..473658(+) (pilM) [Pseudomonas sp. S49]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPANAVVEKNIAELEGVGQALSRVLVKARTGLKGVAV
AVAGSAVITKIIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAATQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTI
EQAGLAKKQGGLPDDYISEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLKTPTQVANP
FSNMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=215744 PspS49_RS02080 WP_127925526.1 472594..473658(+) (pilM) [Pseudomonas sp. S49]
GTGCTGGGACTCTTCAATAAAAAGGCCAATACGTTACTGGGGATCGACATCAGTTCCACGTCAGTGAAGCTGCTGGAACT
GAGCCGTCAGGGTGATCGCTACCGGGTCGAGGCGTACGCGGTCGAGCCATTGCCGGCCAACGCTGTGGTCGAGAAAAACA
TCGCCGAACTCGAAGGCGTTGGCCAGGCCCTGAGTCGGGTGCTGGTCAAGGCGCGTACCGGGCTCAAGGGCGTTGCAGTC
GCAGTGGCCGGTTCGGCGGTGATTACCAAAATCATCGAAATGGACGCCGGGCTGTCCGATGACGAACTGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATTCCCTATCCATTGGACGAAGTTGCCATCGATTTCGAAGTCCAGGGCGTTTCGCCGC
GCAACCCTGAACGGGTCAACGTGCTGCTGGCTGCCTGTCGCAAGGAAAACGTCGAAGTCCGTGAAGCGGCGCTGGCACTC
GCCGGGCTGACTGCGCGGGTCGTCGATGTCGAAGCCTATGCGCTGGAACGCTCTTTCGGGCTGCTCGCCACGCAACTGGC
GGCCACGCAAGAGCGGCTCACTGTCGCCGTCGTCGATATCGGCGCGACCATGACCACCCTCAGCGTTCTGCACAACGGCA
AGATCATCTACACCCGCGAGCAATTGTTCGGCGGGCGGCAACTGACCGAAGAAATCCAGCGCCGTTATGGCCTGACCATC
GAGCAGGCGGGGCTGGCGAAAAAGCAGGGCGGTCTGCCCGATGATTACATCAGCGAGGTCTTGCAGCCGTTTCGTGAAGC
GCTGGTACAGCAAGTGTCGCGCTCGTTGCAGTTCTTCTTCGCCTCCGGGCAATACAACGCGGTCGACCACATTCTGCTGG
CCGGCGGTACGGCCTCAGTGCCGGGGCTGGACCGGCTGATCGAGCAGCGCCTGAAGACGCCGACGCAAGTGGCCAATCCG
TTTTCCAATATGGCCTTGAGCAGCAAGGTCAATGCCGGAGCGCTGGCCAGCGACGCGCCGGCCCTGATGATCGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

47.175

100

0.472

Multiple sequence alignment