Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PchlR47_RS27560 Genome accession   NZ_CP019399
Coordinates   6172929..6174146 (+) Length   405 a.a.
NCBI ID   WP_028683416.1    Uniprot ID   A0AAD0ZKX8
Organism   Pseudomonas chlororaphis strain R47     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6167929..6179146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PchlR47_RS27545 (PchlR47_27700) - 6168383..6171301 (+) 2919 WP_174248210.1 DUF748 domain-containing protein -
  PchlR47_RS27550 (PchlR47_27705) - 6171504..6171860 (-) 357 WP_009050906.1 BON domain-containing protein -
  PchlR47_RS27555 (PchlR47_27710) - 6172148..6172564 (-) 417 WP_009050907.1 pilin -
  PchlR47_RS27560 (PchlR47_27715) pilC 6172929..6174146 (+) 1218 WP_028683416.1 type II secretion system F family protein Machinery gene
  PchlR47_RS27565 (PchlR47_27720) pilD 6174149..6175021 (+) 873 WP_009050909.1 prepilin peptidase Machinery gene
  PchlR47_RS27570 (PchlR47_27725) coaE 6175018..6175641 (+) 624 WP_009045669.1 dephospho-CoA kinase -
  PchlR47_RS27575 (PchlR47_27730) yacG 6175638..6175841 (+) 204 WP_009045670.1 DNA gyrase inhibitor YacG -
  PchlR47_RS27580 (PchlR47_27735) - 6175846..6176061 (-) 216 WP_009050911.1 hypothetical protein -
  PchlR47_RS27585 (PchlR47_27740) - 6176127..6176816 (-) 690 WP_110177023.1 energy-coupling factor ABC transporter permease -
  PchlR47_RS27590 (PchlR47_27745) - 6176813..6177280 (-) 468 WP_009050913.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44435.22 Da        Isoelectric Point: 10.4090

>NTDB_id=215214 PchlR47_RS27560 WP_028683416.1 6172929..6174146(+) (pilC) [Pseudomonas chlororaphis strain R47]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHTPSLIRAQLRKQGISPARVRKKSSSLFQRTPPIKARDISLFTRQLATLIK
AGVPLLQSFDIIAEGFDNPNMRKLLAELKQDIAAGSSFTSALRKRPRYFDELYCSLVDAGEQAGALETLLERVATYKEKS
ENLKTRIRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPVFTQLVIALSEFVQHWWWLLAGGLLLSVFGLR
HAHRRSPRFRQWLDVGLLKLPLVAPLLYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKHAVNRIRQDVATGM
QLHFSMRASGIFPGMAIQMTAIGEESGALDGMLEKVAIHYEEEVDTLVDTLSSLMEPVIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=215214 PchlR47_RS27560 WP_028683416.1 6172929..6174146(+) (pilC) [Pseudomonas chlororaphis strain R47]
ATGGCGGTCAAAGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATACCCCCTCGCTGATCAGGGCCCAGTTGCGCAAGCAAGGCATCAGCCCGGCCAGGGTCAGGAAGAAATCCA
GCTCGCTGTTCCAGCGCACGCCCCCGATCAAGGCTCGCGACATCAGCCTGTTCACCCGGCAGTTGGCGACCCTGATCAAG
GCCGGCGTACCGCTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGATAACCCGAACATGCGCAAACTGCTGGCCGA
ACTGAAACAGGACATCGCCGCCGGCAGCAGTTTCACCAGTGCGCTGCGCAAGAGGCCGCGGTATTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAGCAGGCCGGCGCCCTGGAAACCCTGCTGGAGCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACCCGAATCCGCAAGGCCATGACCTATCCCCTGGCGGTGATCTGCGTGGCGCTGGTGGTGACAGGGAT
CCTGCTGGTCAAGGTGGTGCCGCAATTCCAGTCGATGTTCCAAGGCTTCGGCGCGCAACTGCCGGTGTTCACCCAACTGG
TCATCGCCCTCTCGGAGTTCGTGCAGCACTGGTGGTGGCTGTTGGCGGGCGGTCTGTTGCTCAGCGTATTCGGCCTGCGC
CATGCCCACCGGCGCTCACCGCGCTTTCGCCAGTGGCTGGACGTGGGCCTGCTGAAACTGCCGTTGGTAGCGCCGCTGCT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACCTTCGCCGCCGGGGTACCACTGGTGGAGGCCCTGG
GCTCGGTGGCCGGGGCCACTGGCAACCAGGTGTTCAAGCATGCGGTGAACCGCATCAGGCAGGACGTGGCGACTGGCATG
CAGTTGCATTTTTCCATGCGCGCATCCGGCATTTTTCCCGGCATGGCGATCCAGATGACCGCCATCGGCGAAGAATCCGG
GGCCCTGGATGGCATGCTGGAAAAAGTCGCGATCCACTACGAGGAAGAGGTCGATACCCTGGTCGACACCCTGAGCAGTC
TCATGGAGCCCGTGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGCACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

66.173

100

0.662
  pilC Acinetobacter baylyi ADP1

55.882

100

0.563
  pilC Acinetobacter baumannii D1279779

54.902

100

0.553
  pilC Legionella pneumophila strain ERS1305867

52.778

97.778

0.516
  pilG Neisseria gonorrhoeae MS11

42.432

99.506

0.422
  pilG Neisseria meningitidis 44/76-A

41.687

99.506

0.415
  pilC Vibrio cholerae strain A1552

42.347

96.79

0.41
  pilC Vibrio campbellii strain DS40M4

39.552

99.259

0.393

Multiple sequence alignment