Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PspS34_RS01890 Genome accession   NZ_CP019398
Coordinates   429955..432024 (+) Length   689 a.a.
NCBI ID   WP_154861180.1    Uniprot ID   -
Organism   Pseudomonas sp. S34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 424955..437024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PspS34_RS01870 (PspS34_01875) comM 427167..428231 (+) 1065 WP_154861184.1 pilus assembly protein PilM Machinery gene
  PspS34_RS01875 (PspS34_01880) - 428231..428797 (+) 567 WP_154861183.1 PilN domain-containing protein -
  PspS34_RS01880 (PspS34_01885) pilO 428794..429417 (+) 624 WP_154861182.1 type 4a pilus biogenesis protein PilO -
  PspS34_RS01885 (PspS34_01890) - 429414..429941 (+) 528 WP_154861181.1 pilus assembly protein PilP -
  PspS34_RS01890 (PspS34_01895) pilQ 429955..432024 (+) 2070 WP_154861180.1 type IV pilus secretin PilQ Machinery gene
  PspS34_RS01895 (PspS34_01900) aroK 432029..432547 (+) 519 WP_057712350.1 shikimate kinase AroK -
  PspS34_RS01900 (PspS34_01905) aroB 432699..433799 (+) 1101 WP_057712349.1 3-dehydroquinate synthase -
  PspS34_RS01905 (PspS34_01910) - 433810..435411 (+) 1602 WP_150811959.1 AAA family ATPase -

Sequence

Protein


Download         Length: 689 a.a.        Molecular weight: 74415.55 Da        Isoelectric Point: 5.5369

>NTDB_id=215176 PspS34_RS01890 WP_154861180.1 429955..432024(+) (pilQ) [Pseudomonas sp. S34]
MNRILSTVGLSLWIALLSPMVQAANLKALDVAALPGDRVELKLSFDAPPPVPHGYTTDSPARIALDLPGVASQLASKTHD
LGSGNARSATVVEARDRTRLIINLTQPAPYDSRIEGNNLLVVVGQGVKPSISRPAAVATPVPARAVATAGKAIRGVDFQR
GTQGEGNVVIDLSDPSIAPDIQEREGRIIVGFAKTLLPERLRVRLDVKDFATPVQFVNASASGDRATISIEPGGTFDYST
YQTDNKLTVSVRPATVDELQKRNAGQQAYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQA
LDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSAEAKPDERG
SITVDERTNNIIAYQTQDRLDELRRIVTQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSNGLNGS
STIGTPGSTSTNSPFVDMGVANNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQEA
SSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQS
KVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNRAIAVSH

Nucleotide


Download         Length: 2070 bp        

>NTDB_id=215176 PspS34_RS01890 WP_154861180.1 429955..432024(+) (pilQ) [Pseudomonas sp. S34]
ATGAACAGGATTCTTTCCACCGTCGGCCTTTCGCTATGGATAGCGTTGTTGTCGCCGATGGTACAAGCGGCCAATCTCAA
GGCGCTGGATGTCGCAGCGCTGCCGGGTGACCGTGTCGAGTTGAAGTTGTCGTTCGATGCGCCGCCCCCGGTGCCCCACG
GCTACACGACGGATTCGCCAGCCCGGATTGCCCTGGATTTGCCGGGTGTCGCCAGTCAGCTGGCGAGCAAGACTCACGAT
CTGGGCAGTGGAAATGCCCGAAGTGCGACGGTGGTGGAAGCCCGGGACCGTACGCGATTGATCATCAACCTGACCCAGCC
GGCCCCGTACGACTCGCGAATAGAAGGTAACAATCTGCTGGTTGTTGTCGGGCAGGGAGTCAAACCGTCGATATCCAGGC
CGGCGGCGGTAGCAACGCCGGTTCCGGCACGAGCCGTTGCGACGGCGGGCAAGGCCATTCGGGGTGTGGATTTCCAGCGG
GGGACCCAGGGCGAAGGCAACGTGGTGATCGACTTGTCGGATCCATCGATTGCGCCCGACATACAAGAGCGCGAAGGCAG
GATCATCGTCGGATTTGCCAAGACCTTATTGCCCGAGCGATTGCGCGTGCGCCTGGACGTGAAGGATTTCGCCACGCCCG
TGCAGTTCGTCAATGCCAGCGCGAGCGGCGATCGAGCCACCATCAGCATTGAGCCCGGCGGCACGTTCGATTACTCGACC
TATCAGACTGACAATAAGCTCACGGTCAGCGTTCGCCCGGCGACGGTCGACGAACTGCAAAAACGCAATGCCGGGCAACA
GGCCTACAGTGGCGAGAAGCTATCGCTGAACTTCCAGGACATCGATGTGCGCTCGGTGCTGCAACTGATCGCCGATTTCA
CCAACCTCAATCTGGTGGCCAGCGACACGGTCCAGGGGGGAATTACCCTGCGCTTGCAGAACGTGCCGTGGGATCAGGCG
CTGGATCTGGTGCTGAAAACCAAGGGGCTGGACAAGCGCAAGGTCGGCAATGTCCTGCTGGTTGCGCCGGCCGATGAAAT
TGCTGCCCGTGAGCGTCAGGAGCTCGAATCGCAGAAGCAGATTGCCGATCTGGCGCCGTTGCGTCGGGAGCTGTTGCAGG
TCAATTACGCCAAGGCGGCGGATATCGCCAAGCTCTTCCAGTCGGTGACCAGTGCCGAAGCGAAACCCGACGAACGGGGA
TCAATCACCGTCGATGAACGCACCAACAACATCATTGCCTACCAGACCCAGGACCGGCTGGATGAGCTGCGCAGGATCGT
CACGCAGCTGGATATCCCTGTGCGTCAGGTGATGATCGAGGCGCGCATTGTCGAAGCCAACGTCGACTATGACAAAAGCC
TCGGGGTGCGCTGGGGTGGTTCGATACAGAACAAGGGCAACTGGAACACCTCCGGCGTCAGCAATGGACTCAATGGGTCA
TCGACCATTGGCACGCCCGGCAGTACCAGCACCAACTCACCGTTCGTCGACATGGGCGTAGCCAACAACACCTCGGGAAT
CGGCATCGCCTTCATCACCGATAACGTCTTGCTGGACCTGGAGCTGACGGCCATGGAGAAAACCGGCAACGGGGAAATCG
TCTCGCAGCCCAAGGTGGTCACGTCCGACAAGGAAACCGCCAAAATCCTCAAGGGCACGGAAATTCCCTATCAGGAAGCC
AGCTCCAGTGGCGCGACTTCGGTGTCGTTCAAGGAGGCGTCGCTGTCGCTGGAGGTGACGCCGCAGATCACGCCCGACAA
CCGGATCATCATGGAAGTCAAGGTGACCAAGGACGAACCGGACTACCTGAACAAGGTTCAGGATGTACCCCCCATCAAGA
AAAACGAAGTCAACGCCAAGGTCCTGGTCAACGATGGCGAGACCATCGTCATTGGTGGTGTTTTCTCAAATACTCAGAGC
AAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTTCGGAGAA
AAAATCCGAGCTGTTGGTATTCCTCACTCCGCGTATCATGAATAATCGGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(371-436)

Secretin and TonB N terminus short domain (STN)

(297-343)

AMIN domain (AMIN)

(38-121)

Bacterial type II and III secretion system protein (Secretin)

(524-680)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.95

100

0.737
  comQ Acinetobacter baylyi ADP1

40.955

100

0.411
  pilQ Acinetobacter baumannii D1279779

37.65

100

0.409
  pilQ Acinetobacter baumannii strain A118

37.65

100

0.409
  pilQ Legionella pneumophila strain Lp02

38.519

97.968

0.377
  pilQ Legionella pneumophila strain ERS1305867

38.37

97.968

0.376

Multiple sequence alignment