Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   PspS34_RS01870 Genome accession   NZ_CP019398
Coordinates   427167..428231 (+) Length   354 a.a.
NCBI ID   WP_154861184.1    Uniprot ID   -
Organism   Pseudomonas sp. S34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 422167..433231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PspS34_RS01860 (PspS34_01865) - 422977..424245 (+) 1269 WP_008047852.1 malic enzyme-like NAD(P)-binding protein -
  PspS34_RS01865 (PspS34_01870) - 424511..426952 (-) 2442 WP_154861185.1 penicillin-binding protein 1A -
  PspS34_RS01870 (PspS34_01875) comM 427167..428231 (+) 1065 WP_154861184.1 pilus assembly protein PilM Machinery gene
  PspS34_RS01875 (PspS34_01880) - 428231..428797 (+) 567 WP_154861183.1 PilN domain-containing protein -
  PspS34_RS01880 (PspS34_01885) pilO 428794..429417 (+) 624 WP_154861182.1 type 4a pilus biogenesis protein PilO -
  PspS34_RS01885 (PspS34_01890) - 429414..429941 (+) 528 WP_154861181.1 pilus assembly protein PilP -
  PspS34_RS01890 (PspS34_01895) pilQ 429955..432024 (+) 2070 WP_154861180.1 type IV pilus secretin PilQ Machinery gene
  PspS34_RS01895 (PspS34_01900) aroK 432029..432547 (+) 519 WP_057712350.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37995.69 Da        Isoelectric Point: 4.8438

>NTDB_id=215175 PspS34_RS01870 WP_154861184.1 427167..428231(+) (comM) [Pseudomonas sp. S34]
MPGLFKKKSNTLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPVGAVIEKNIAELEGVGLALTRALAKARTALKNVAV
AVAGSAVITKTLEMDAGLCEDEMESQLKIEADQYIPYPLDEVAIDFEVQGTSGRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFELLASRLAVSEKPLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAQKKGGLPDDYAAEVLQPFREALVQQVSRSLHFFLASGQYSAVDHVLLAGGTASIPGLDRLIEQQLGTPTQVANP
FCEMTLGSKVNGAALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=215175 PspS34_RS01870 WP_154861184.1 427167..428231(+) (comM) [Pseudomonas sp. S34]
GTGCCAGGACTCTTCAAGAAAAAATCCAATACGCTGCTGGGAATCGACATCAGTTCCACCTCGGTGAAACTTCTTGAGTT
GAGCCGTCAGGGTGAGCGCTACCGGGTCGAGGCCTATGCGGTCGAGCCATTGCCGGTCGGCGCGGTGATCGAAAAGAATA
TCGCCGAGCTCGAAGGTGTGGGGCTGGCCCTCACTCGCGCGCTGGCCAAGGCCAGAACCGCCCTCAAGAACGTGGCCGTG
GCCGTGGCGGGCTCAGCGGTGATCACCAAGACCCTCGAGATGGATGCCGGTCTTTGCGAAGACGAAATGGAAAGCCAGCT
GAAAATCGAAGCTGACCAGTACATTCCTTATCCCCTGGACGAAGTCGCGATCGACTTTGAAGTCCAGGGCACCTCCGGGC
GTAATCCCGAGCGCGTCAACGTGCTGCTCGCCGCTTGTCGCAAGGAAAACGTCGAAGTCCGCGAGGCCGCCCTGGCCCTG
GCGGGGCTGACCGCCCGCGTGGTCGATGTCGAGGCCTACGCGCTGGAGCGTTCATTCGAACTTCTGGCCAGTCGACTCGC
GGTTTCCGAGAAACCTTTGACGGTCGCAGTGGTGGACATCGGCGCCACCATGACCACCCTGAGCGTGCTGCACAACGGGC
GGATTATCTACACCCGCGAACAACTGTTCGGCGGTCGGCAACTGACCGAGGAAATCCAGCGTCGCTACGGCCTGACGCTG
GAGCAGGCGGGGCTGGCGCAAAAGAAGGGCGGGTTGCCGGATGACTATGCCGCTGAGGTCTTGCAGCCATTTCGAGAGGC
GCTGGTGCAGCAGGTGTCGCGCTCGTTGCACTTCTTCCTTGCTTCCGGCCAGTACAGCGCCGTCGACCATGTCCTGCTGG
CCGGCGGTACGGCGTCGATCCCGGGGCTGGACCGTTTGATCGAGCAGCAATTGGGCACCCCGACCCAGGTCGCGAACCCG
TTTTGCGAAATGACTCTGGGCAGCAAGGTCAATGGCGCTGCCCTGGCGAGTGATGCCCCCGCGTTGATGATCGCCTGCGG
TCTGGCCCTCAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

54.701

99.153

0.542
  pilM Acinetobacter baumannii D1279779

54.416

99.153

0.54
  comM Acinetobacter baylyi ADP1

52.542

100

0.525
  pilM Legionella pneumophila strain ERS1305867

47.175

100

0.472

Multiple sequence alignment