Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BWP24_RS02810 Genome accession   NZ_CP019293
Coordinates   563010..564695 (-) Length   561 a.a.
NCBI ID   WP_045421881.1    Uniprot ID   -
Organism   Vibrio campbellii strain LMB29     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 558010..569695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWP24_RS02780 (BWP24_02820) rplS 558208..558561 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  BWP24_RS02785 (BWP24_02825) yacG 559170..559370 (-) 201 WP_012128882.1 DNA gyrase inhibitor YacG -
  BWP24_RS02790 (BWP24_02830) zapD 559427..560167 (-) 741 WP_012128881.1 cell division protein ZapD -
  BWP24_RS02795 (BWP24_02835) coaE 560195..560809 (-) 615 WP_045369833.1 dephospho-CoA kinase -
  BWP24_RS02800 (BWP24_02840) pilD 560810..561679 (-) 870 WP_045381296.1 A24 family peptidase Machinery gene
  BWP24_RS02805 (BWP24_02845) pilC 561752..562975 (-) 1224 WP_077200206.1 type II secretion system F family protein Machinery gene
  BWP24_RS02810 (BWP24_02850) pilB 563010..564695 (-) 1686 WP_045421881.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BWP24_RS02815 (BWP24_02855) pilA 564695..565111 (-) 417 WP_045381308.1 type IV pilin protein Machinery gene
  BWP24_RS02825 (BWP24_02865) nadC 565376..566263 (-) 888 WP_077200208.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BWP24_RS02830 (BWP24_02870) ampD 566356..566907 (+) 552 WP_077200209.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BWP24_RS02835 (BWP24_02875) pdhR 567312..568079 (+) 768 WP_005428674.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62046.79 Da        Isoelectric Point: 5.2806

>NTDB_id=214640 BWP24_RS02810 WP_045421881.1 563010..564695(-) (pilB) [Vibrio campbellii strain LMB29]
MHTNLSTVLRQAGILTFSQEESLLEQAKASGISMPEALLSSGFFQSHELTEHLSSIFGLSCTSLSQYEYASLCQTLGLRD
LITRHNALPLNRTSSALILAVADPTNLQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRSLGQEKSGLKEINQDELAG
LVDIGEDEIENIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYENMYRVRLRCDGILIEIQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSAALDINKLGYSDSQKQLYLNALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLPGINQVQVQPKIGFGFAEALRSFLRQDPDIVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCPYCRQPQEPNSQLQHIGITSTEQ
IFQANPDGCNECTHGYSGRTGIYEVMKFDESLSEALIKGASVHELEKLAIANGMQTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=214640 BWP24_RS02810 WP_045421881.1 563010..564695(-) (pilB) [Vibrio campbellii strain LMB29]
ATGCACACAAACCTCTCAACGGTTCTTCGTCAAGCGGGAATACTGACTTTTTCCCAAGAAGAATCGTTACTTGAGCAAGC
TAAAGCTTCTGGCATTTCGATGCCGGAAGCTTTGCTCAGTTCTGGCTTTTTCCAATCTCATGAATTGACTGAGCACCTGA
GCTCTATCTTTGGGCTTTCCTGCACGTCACTCAGTCAGTATGAATACGCTTCGCTATGCCAAACGCTTGGCTTACGCGAT
CTCATCACTCGCCATAACGCTTTGCCGCTTAACCGCACCTCTTCAGCTTTGATTCTCGCGGTTGCCGACCCAACAAACCT
TCAAGCCGAAGATGACTTTCGTTTTGCGACAGGTTTGCAAGTCGAACTTGTCCTCGCTGACTTTCGTGAGCTAACAGCAG
CTATTCGGCGTTTATATGGCCGATCGCTTGGGCAAGAAAAATCCGGTCTTAAAGAAATCAACCAAGATGAACTGGCGGGT
TTGGTTGATATTGGTGAGGATGAAATTGAGAACATCGAAGATCTCAGCCAAGATGAATCGCCGGTCAGTCGCTATATCAA
CCAAATTTTACTCGATGCTGTCCGTAAAGGGGCTTCCGATATTCACTTCGAACCCTACGAAAACATGTACCGCGTGCGCT
TGCGTTGCGATGGCATCTTAATTGAAATTCAGCAGCCTCCGAGTCATTTAAGCCGTCGCCTTTCAGCTCGGATAAAAATC
TTATCTAAGCTTGATATCGCCGAGCGCCGTTTACCACAGGATGGGCGCATCAAGCTCAAATTAAACCAAGACACTGCAAT
TGATATGCGTGTCTCCACACTACCAACTCTTTTTGGGGAAAAGATAGTTCTGCGTTTGCTCGACAGCAGTTCTGCAGCAT
TAGATATCAATAAGCTCGGCTATAGCGACTCACAAAAACAACTTTATTTGAATGCCCTGCGACGACCTCAAGGTATGATC
CTGATGACAGGCCCAACGGGTAGCGGTAAAACGGTCTCGCTCTATACTGGATTGAGTATTCTTAATAAGCCAGAGATCAA
TATCTCAACCGCTGAAGATCCCGTTGAGATTAACCTGCCCGGCATTAACCAAGTTCAAGTTCAGCCAAAGATCGGCTTTG
GTTTTGCAGAAGCGCTGCGCTCTTTCTTACGTCAAGACCCTGATATAGTCATGGTGGGCGAGATCCGAGATCTCGACACA
GCAGAAATCGCGATCAAAGCATCACAGACTGGCCACTTAGTTCTCTCCACTTTGCACACGAACTCAGCTGCCGAGACTGT
CATTCGCTTGTCTAATATGGGCGTAGAAAGCTTTAACCTCGCCTCCTCTCTCAGTTTGATCATCGCCCAACGTTTGGCTA
GAAAACTTTGTCCATATTGTAGACAACCTCAAGAGCCAAACAGCCAACTTCAACATATTGGAATCACCAGCACCGAACAG
ATCTTTCAAGCTAATCCCGACGGTTGTAACGAGTGTACCCATGGTTATTCAGGGCGAACCGGCATTTACGAAGTGATGAA
GTTTGACGAATCCCTTTCAGAAGCACTCATCAAAGGCGCTTCAGTACACGAATTAGAAAAACTCGCCATTGCCAATGGTA
TGCAAACCCTACAAATGTCTGGCATCGAAAAACTCAAACAAGGCATTACCAGCTTCAGCGAATTGCAGCGCGTTCTCTAC
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

98.039

100

0.98
  pilB Vibrio parahaemolyticus RIMD 2210633

90.731

100

0.907
  pilB Vibrio cholerae strain A1552

73.665

100

0.738
  pilB Acinetobacter baylyi ADP1

48.115

99.287

0.478
  pilB Acinetobacter baumannii D1279779

51.445

92.513

0.476
  pilB Legionella pneumophila strain ERS1305867

48.524

96.613

0.469
  pilF Neisseria gonorrhoeae MS11

43.617

100

0.439
  pilB/pilB1 Synechocystis sp. PCC 6803

36.869

100

0.39
  pilF Thermus thermophilus HB27

37.011

100

0.371
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.738

99.465

0.365

Multiple sequence alignment