Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MCA_RS10260 Genome accession   NC_002977
Coordinates   2252675..2254390 (-) Length   571 a.a.
NCBI ID   WP_010961339.1    Uniprot ID   Q606C2
Organism   Methylococcus capsulatus str. Bath     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2247675..2259390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MCA_RS10235 (MCA2090) yacG 2248861..2249076 (-) 216 WP_017366376.1 DNA gyrase inhibitor YacG -
  MCA_RS10240 (MCA2091) zapD 2249090..2249857 (-) 768 WP_010961334.1 cell division protein ZapD -
  MCA_RS10245 (MCA2093) coaE 2249959..2250555 (-) 597 WP_010961336.1 dephospho-CoA kinase -
  MCA_RS10250 (MCA2094) pilD 2250563..2251432 (-) 870 WP_010961337.1 prepilin peptidase Machinery gene
  MCA_RS10255 (MCA2095) pilC 2251456..2252670 (-) 1215 WP_010961338.1 type II secretion system F family protein Machinery gene
  MCA_RS10260 (MCA2096) pilB 2252675..2254390 (-) 1716 WP_010961339.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MCA_RS10265 (MCA2097) - 2254440..2257205 (-) 2766 WP_010961340.1 valine--tRNA ligase -
  MCA_RS10270 (MCA2098) - 2257273..2257731 (-) 459 WP_010961341.1 DNA polymerase III subunit chi -
  MCA_RS10275 (MCA2099) - 2257728..2259221 (-) 1494 WP_010961342.1 leucyl aminopeptidase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63100.82 Da        Isoelectric Point: 5.5480

>NTDB_id=21451 MCA_RS10260 WP_010961339.1 2252675..2254390(-) (pilB) [Methylococcus capsulatus str. Bath]
MAAVSGVQQLSGLAKSLVKAQLLSEADAQAYFEDARRKNTPFVSYLVANKILDGLQIAQVAHQEFGLPLLDLDAVVFDIP
PTQWVGEKLIRRHHILPLLRRGNHLFVAVSDPTNFLGLDEITFQTRLVTDCVLVEENKLSRCIEAVLDAADTSLKQLLEE
DLDSLQITAGDDEGPHAEPEISGIDDAPIVRFVNRLLLDAVRKGASDIHIEPYEKFLRIRFRHDGILHEVANPPPALGVK
ICARIKVMSRMDIAEHRIPQDGRIKMTLSRSRAIDFRVNTCPTLFGEKIVLRILDPSSAQIGVEMLGFEPEQRDNFLRAL
EKPYGMILVTGPTGSGKTVTLYTGLNLLNRVEANISTVEDPVEITVPGINQVNVNYKTGLTFAEALRAFLRQDPDIIMVG
EIRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLNRLMQMGIPAFNIASSVLLIMAQRLARRLCPRCKREEKVPPEVLLA
SGFREEDIGNFTLFGPAGCDQCVKGYKGRVGIYQVMPISEEINRIILEGGNVMALTEQAHAEGIADLRESGLKKVKAGIT
SLEEIDRVTRD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=21451 MCA_RS10260 WP_010961339.1 2252675..2254390(-) (pilB) [Methylococcus capsulatus str. Bath]
ATGGCGGCGGTTTCCGGCGTCCAGCAGCTGAGCGGTCTGGCGAAAAGCCTGGTCAAGGCCCAGTTGCTTTCGGAGGCGGA
CGCCCAGGCGTACTTCGAGGATGCGCGCAGGAAGAACACCCCTTTCGTCAGCTATCTCGTTGCCAACAAGATCCTGGACG
GTCTCCAGATCGCCCAGGTCGCCCACCAGGAGTTCGGGCTGCCCCTGCTCGATCTGGACGCAGTCGTCTTCGACATTCCC
CCCACGCAGTGGGTCGGCGAGAAGCTGATACGCCGCCATCACATCCTGCCCTTGCTGCGACGGGGGAATCATCTCTTCGT
CGCGGTCTCGGATCCGACCAATTTCCTGGGACTGGATGAAATCACTTTCCAGACCCGTCTCGTTACCGACTGTGTCCTGG
TCGAGGAGAACAAGCTTTCGCGCTGCATCGAGGCGGTCCTGGATGCGGCGGATACCAGCCTGAAGCAGCTGCTCGAAGAG
GATCTGGACAGTCTGCAGATCACCGCCGGGGACGACGAAGGCCCGCACGCCGAGCCGGAAATCTCCGGTATCGACGATGC
GCCGATCGTGCGCTTCGTCAACCGCCTTCTGCTGGACGCGGTGCGGAAGGGGGCCTCGGACATCCATATCGAGCCTTACG
AAAAGTTCCTGCGCATCCGCTTCCGGCACGACGGCATCCTGCATGAGGTGGCGAATCCGCCGCCTGCGCTGGGCGTCAAG
ATCTGCGCTCGCATCAAAGTGATGTCGCGCATGGATATCGCGGAACACCGCATTCCCCAGGACGGACGCATCAAGATGAC
GCTGTCGCGCTCCCGCGCCATCGATTTCCGCGTCAATACCTGTCCGACGCTGTTCGGGGAAAAGATCGTGCTGCGTATCC
TCGATCCGAGCTCGGCCCAGATCGGCGTCGAAATGCTGGGATTCGAGCCGGAACAGCGGGACAATTTCCTGCGTGCGCTG
GAAAAGCCCTATGGCATGATCCTGGTTACCGGCCCCACCGGCAGCGGTAAAACCGTTACCCTCTACACCGGTCTGAACCT
CCTCAACCGCGTCGAAGCGAACATCTCCACGGTGGAGGACCCGGTCGAGATCACAGTGCCGGGGATCAACCAGGTCAATG
TCAATTACAAGACCGGCCTCACTTTCGCCGAGGCGCTGCGGGCGTTTCTGCGCCAGGACCCCGACATCATCATGGTGGGC
GAGATCCGCGACCTGGAAACCGCCGAAATCGCCGTCAAGGCGGCCCAGACCGGGCATCTGGTGCTGTCGACCCTGCACAC
CAACGATGCCCCGCAGACCTTGAACCGTCTGATGCAGATGGGGATCCCCGCGTTCAACATCGCCTCCTCCGTGCTCCTGA
TCATGGCGCAGCGGCTGGCCCGCCGGCTTTGTCCGCGGTGCAAGCGGGAGGAGAAAGTGCCGCCCGAGGTCCTGCTCGCC
TCCGGCTTCCGGGAGGAAGACATCGGCAATTTCACGCTGTTCGGGCCGGCGGGCTGCGATCAATGTGTGAAGGGCTACAA
GGGGCGGGTCGGCATTTACCAGGTCATGCCGATTTCGGAAGAAATCAACCGCATCATCCTGGAGGGAGGGAACGTGATGG
CCCTGACCGAGCAGGCGCATGCGGAAGGTATCGCGGATCTGAGGGAGTCTGGCTTGAAGAAGGTGAAGGCGGGTATCACC
AGCCTCGAGGAAATCGACCGCGTGACGAGGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q606C2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.652

100

0.56
  pilB Acinetobacter baylyi ADP1

52.397

100

0.536
  pilB Acinetobacter baumannii D1279779

51.986

100

0.527
  pilB Vibrio cholerae strain A1552

48.845

98.599

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

48.221

98.424

0.475
  pilF Neisseria gonorrhoeae MS11

47.518

98.774

0.469
  pilB Vibrio campbellii strain DS40M4

47.163

98.774

0.466
  pilF Thermus thermophilus HB27

37.786

99.65

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

40.232

90.543

0.364

Multiple sequence alignment