Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   MCA_RS10250 Genome accession   NC_002977
Coordinates   2250563..2251432 (-) Length   289 a.a.
NCBI ID   WP_010961337.1    Uniprot ID   Q606C4
Organism   Methylococcus capsulatus str. Bath     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2245563..2256432
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MCA_RS15740 (MCA2086) - 2245711..2246286 (-) 576 WP_010961329.1 RNA pyrophosphohydrolase -
  MCA_RS10220 (MCA2087) - 2246474..2247136 (+) 663 WP_010961330.1 HAD family hydrolase -
  MCA_RS10225 (MCA2088) - 2247145..2247375 (-) 231 WP_010961331.1 sulfurtransferase TusA family protein -
  MCA_RS10230 (MCA2089) cysG 2247424..2248848 (+) 1425 WP_010961332.1 siroheme synthase CysG -
  MCA_RS10235 (MCA2090) yacG 2248861..2249076 (-) 216 WP_017366376.1 DNA gyrase inhibitor YacG -
  MCA_RS10240 (MCA2091) zapD 2249090..2249857 (-) 768 WP_010961334.1 cell division protein ZapD -
  MCA_RS10245 (MCA2093) coaE 2249959..2250555 (-) 597 WP_010961336.1 dephospho-CoA kinase -
  MCA_RS10250 (MCA2094) pilD 2250563..2251432 (-) 870 WP_010961337.1 prepilin peptidase Machinery gene
  MCA_RS10255 (MCA2095) pilC 2251456..2252670 (-) 1215 WP_010961338.1 type II secretion system F family protein Machinery gene
  MCA_RS10260 (MCA2096) pilB 2252675..2254390 (-) 1716 WP_010961339.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 289 a.a.        Molecular weight: 31182.90 Da        Isoelectric Point: 7.5685

>NTDB_id=21449 MCA_RS10250 WP_010961337.1 2250563..2251432(-) (pilD) [Methylococcus capsulatus str. Bath]
MMLLPTGPAGVALCGSFGLIIGSFLNVVIHRLPLMMEQAWRRECGELLEPGTGRPVGKAEYNLWRPGSQCPHCQAPVRFW
QNVPVFSYLWLRGRCAACGTAISWRYPLVETLTGVLFAMVAWHFGASWQALAACALTAGLIALAFIDLDHLVLPDDITLP
LLWLGLLLNAQGLFCSLETALYGAVAGYLLLWTVHRGFLLLTGREGMGFGDFKLLAMLGAWMGWPMLPVIILFSSISGAV
IGSVALAVGGKGRDTPIPFGPFLAAGGWIALLWGNALNDAYWRWAAPGG

Nucleotide


Download         Length: 870 bp        

>NTDB_id=21449 MCA_RS10250 WP_010961337.1 2250563..2251432(-) (pilD) [Methylococcus capsulatus str. Bath]
ATGATGTTGTTGCCGACCGGGCCGGCGGGGGTTGCCCTCTGCGGATCGTTCGGACTGATCATCGGGAGTTTTCTCAACGT
GGTCATCCACCGCCTGCCGCTCATGATGGAGCAGGCCTGGCGCAGGGAGTGCGGGGAGTTGCTCGAGCCCGGCACCGGAC
GGCCGGTGGGGAAAGCCGAATACAACCTGTGGAGACCCGGATCGCAGTGTCCGCATTGCCAGGCGCCGGTCCGGTTCTGG
CAGAATGTCCCCGTGTTCAGCTATCTTTGGCTGCGTGGCCGTTGTGCCGCTTGCGGCACGGCGATTTCCTGGCGTTATCC
TTTGGTCGAAACGCTCACGGGGGTGCTGTTCGCAATGGTGGCTTGGCATTTCGGCGCGTCCTGGCAGGCGCTGGCGGCGT
GCGCCCTAACGGCTGGCCTGATCGCCCTGGCTTTCATCGACCTGGATCACTTGGTACTGCCGGACGACATCACGCTTCCC
CTGTTGTGGCTGGGGCTGCTGCTGAACGCCCAAGGCCTCTTCTGCAGTCTGGAGACGGCGCTGTACGGTGCCGTTGCAGG
CTATCTCCTGCTATGGACGGTGCATCGCGGCTTTCTGCTCCTGACCGGCCGGGAGGGCATGGGGTTCGGCGACTTCAAGC
TACTCGCCATGCTCGGTGCCTGGATGGGTTGGCCGATGCTGCCGGTCATCATCCTGTTCTCATCCATCTCGGGTGCGGTG
ATCGGTTCGGTTGCGCTGGCAGTCGGCGGCAAGGGGAGGGACACGCCGATTCCCTTCGGGCCGTTTCTGGCGGCGGGTGG
CTGGATCGCCCTGCTGTGGGGAAATGCGCTCAACGACGCCTATTGGCGCTGGGCCGCTCCTGGAGGCTAG

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(135-242)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q606C4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

50.36

96.194

0.484
  pilD Vibrio cholerae strain A1552

52.239

92.734

0.484
  pilD Vibrio campbellii strain DS40M4

48.352

94.464

0.457
  pilD Acinetobacter baumannii D1279779

49.057

91.696

0.45
  pilD Acinetobacter nosocomialis M2

48.302

91.696

0.443

Multiple sequence alignment