Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RS694_RS16035 Genome accession   NZ_CP019239
Coordinates   3366232..3367965 (+) Length   577 a.a.
NCBI ID   WP_029707425.1    Uniprot ID   -
Organism   Rhodoferax saidenbachensis strain DSM 22694     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3361232..3372965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS694_RS16005 (RS694_16005) - 3361332..3361859 (+) 528 WP_051391852.1 CNP1-like family protein -
  RS694_RS16010 (RS694_16010) proB 3361870..3363006 (-) 1137 WP_029707432.1 glutamate 5-kinase -
  RS694_RS16015 (RS694_16015) cgtA 3363062..3364141 (-) 1080 WP_029707431.1 Obg family GTPase CgtA -
  RS694_RS16020 (RS694_16020) rpmA 3364280..3364540 (-) 261 WP_029707429.1 50S ribosomal protein L27 -
  RS694_RS16025 (RS694_16025) rplU 3364553..3364867 (-) 315 WP_029707428.1 50S ribosomal protein L21 -
  RS694_RS16030 (RS694_16030) - 3365074..3366003 (+) 930 WP_081708612.1 polyprenyl synthetase family protein -
  RS694_RS16035 (RS694_16035) pilB 3366232..3367965 (+) 1734 WP_029707425.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RS694_RS16040 (RS694_16040) pilC 3367985..3369202 (+) 1218 WP_029707424.1 type II secretion system F family protein Machinery gene
  RS694_RS16045 (RS694_16045) pilD 3369202..3370050 (+) 849 WP_029707423.1 prepilin peptidase Machinery gene
  RS694_RS16050 (RS694_16050) coaE 3370065..3370664 (+) 600 WP_029707422.1 dephospho-CoA kinase -
  RS694_RS16055 (RS694_16055) zapD 3370687..3371442 (+) 756 WP_029707421.1 cell division protein ZapD -
  RS694_RS16060 (RS694_16060) - 3371459..3371662 (+) 204 WP_076069821.1 DNA gyrase inhibitor YacG -
  RS694_RS16065 (RS694_16065) - 3371714..3372202 (-) 489 WP_029707420.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62711.06 Da        Isoelectric Point: 6.0973

>NTDB_id=214341 RS694_RS16035 WP_029707425.1 3366232..3367965(+) (pilB) [Rhodoferax saidenbachensis strain DSM 22694]
MVAVDTLLRDSNPIALPGLGRALVAAGKLGQKSAEEIYRKALSSKNSFIAELVGSGAVSASDLAHTLSTAFAAPLVDLDA
IDSQRLPKGLLDGKICHDYRVVVLSKRNNRLIVATADPSDQAAAEKIKFSTQMGVDWVIAEYDKLSKLAEASAVTATEAM
EDIIGGDFEFDENAAEAVTENAQTNVAEVEDAPVVRFLHKMLMDAFSMRASDLHFEPYEHTYRVRFRIDGELREIASPPI
AIKDKLASRIKVISRMDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKER
LLKAVGRPYGMILVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAAALKSFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPNCKAPVEIPR
NALLDAGFEEKELDGTWKTYHPVGCSMCNNGYKGRVGIYQVMPISDEIQRIILRDGSAMDIAAQAKAEGVRSLRQSGLHK
VKQGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=214341 RS694_RS16035 WP_029707425.1 3366232..3367965(+) (pilB) [Rhodoferax saidenbachensis strain DSM 22694]
ATGGTAGCTGTAGACACTCTTTTGCGTGATTCCAATCCTATTGCTCTACCCGGGTTAGGGCGCGCCTTGGTTGCAGCAGG
GAAGTTGGGACAAAAGTCAGCAGAAGAGATATACCGCAAGGCTCTGTCCAGCAAAAACAGCTTCATTGCAGAACTCGTCG
GCTCGGGCGCAGTATCTGCCTCTGACTTAGCCCACACCTTGTCCACTGCATTTGCGGCCCCGTTGGTAGATCTTGATGCA
ATTGACTCCCAGCGACTCCCCAAGGGCTTACTGGACGGGAAAATATGCCATGACTACCGGGTCGTGGTACTGAGCAAACG
CAATAACCGGCTGATAGTCGCTACAGCGGATCCATCAGATCAAGCTGCAGCGGAAAAGATCAAATTCTCGACGCAAATGG
GCGTTGACTGGGTCATTGCCGAATACGACAAATTATCCAAACTGGCGGAAGCCAGCGCGGTGACCGCCACCGAAGCGATG
GAAGACATCATCGGTGGAGACTTTGAGTTTGATGAAAATGCCGCAGAGGCAGTTACTGAGAACGCCCAGACCAATGTCGC
CGAGGTAGAGGACGCACCGGTCGTGCGCTTCCTCCACAAAATGTTGATGGATGCCTTCAGCATGCGGGCCTCAGATCTTC
ATTTCGAACCCTATGAGCACACCTATCGGGTGCGTTTTCGCATTGATGGTGAGCTGCGTGAAATAGCGAGCCCGCCCATT
GCCATCAAGGACAAGCTGGCCTCCCGGATCAAAGTCATCTCCCGCATGGACATTTCGGAGAAGCGTGTTCCCCAGGACGG
CCGGATGAAACTCAAGGTCGGACCAGACCGCGTCATCGATTTTCGTGTCAGTACCCTGCCGACCCTCTTCGGCGAGAAAA
TCGTCATCCGGATTCTGGATCCCAGCAGCGCAAAACTCGGCATTGATGCCCTAGGTTATGAACCGGAAGAGAAAGAGCGC
CTACTCAAGGCCGTCGGACGCCCTTACGGAATGATCTTGGTAACCGGTCCCACGGGCTCCGGCAAAACGGTATCGCTTTA
CACCTGTCTAAATTTGCTAAACCAGCCGGGCGTGAACATTGCCACGGCGGAAGACCCCTCGGAAATCAACTTACCGGGCG
TAAACCAGGTCAATGTCAACGAAAAAGCGGGCCTGACTTTTGCTGCTGCTTTGAAGTCATTTCTGCGCCAGGATCCGGAC
ATCATCATGGTCGGCGAAATTCGGGATCTGGAAACGGCGGATATTTCCATCAAGGCTGCGCAAACCGGGCATCTGGTGCT
GTCCACCCTGCACACCAACGATGCCCCTACCACCTTGACACGCATGCGCAATATGGGCATTGCGCCGTTCAATATTGCGT
CCAGCGTTATTCTGATTACTGCACAACGCCTTGCACGTCGACTTTGCCCCAACTGTAAGGCACCGGTCGAAATACCTCGT
AATGCTCTGCTGGATGCGGGCTTTGAAGAAAAGGAGCTCGACGGAACCTGGAAGACTTACCATCCGGTCGGATGCTCCAT
GTGCAACAACGGCTACAAGGGGCGCGTTGGCATTTACCAAGTAATGCCCATCAGCGATGAGATCCAGCGCATCATTTTGC
GAGACGGTAGCGCGATGGACATTGCTGCACAAGCCAAAGCAGAAGGCGTTCGCTCTTTGCGACAATCCGGACTGCACAAA
GTAAAACAAGGCCTGACTTCATTGGAAGAAGTCCTGGCAGTGACCAACGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.545

97.227

0.53
  pilB Acinetobacter baylyi ADP1

53.345

98.44

0.525
  pilF Neisseria gonorrhoeae MS11

52.931

97.574

0.516
  pilB Legionella pneumophila strain ERS1305867

50.265

98.267

0.494
  pilB Vibrio campbellii strain DS40M4

44.64

98.614

0.44
  pilB Vibrio parahaemolyticus RIMD 2210633

44.112

98.614

0.435
  pilB Vibrio cholerae strain A1552

46.476

90.988

0.423
  pilF Thermus thermophilus HB27

39.123

98.787

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.076

91.681

0.367

Multiple sequence alignment