Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BVG18_RS01445 Genome accession   NZ_CP019143
Coordinates   312915..314624 (-) Length   569 a.a.
NCBI ID   WP_086044367.1    Uniprot ID   -
Organism   Acinetobacter lwoffii strain ZS207     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 307915..319624
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVG18_RS01415 (BVG18_07035) - 308034..308357 (+) 324 WP_004281089.1 pyrimidine/purine nucleoside phosphorylase -
  BVG18_RS01420 (BVG18_07040) rlmB 308492..309241 (+) 750 WP_004281091.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  BVG18_RS01425 (BVG18_07045) - 309245..310171 (+) 927 WP_016806884.1 DMT family transporter -
  BVG18_RS01430 (BVG18_07050) coaE 310193..310792 (-) 600 WP_004281093.1 dephospho-CoA kinase -
  BVG18_RS01435 (BVG18_07055) pilD 310794..311654 (-) 861 WP_004281094.1 A24 family peptidase Machinery gene
  BVG18_RS01440 (BVG18_07060) pilC 311654..312883 (-) 1230 WP_004281096.1 type II secretion system F family protein Machinery gene
  BVG18_RS01445 (BVG18_07065) pilB 312915..314624 (-) 1710 WP_086044367.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BVG18_RS01450 (BVG18_07070) tpiA 314902..315693 (+) 792 WP_086044368.1 triose-phosphate isomerase -
  BVG18_RS01455 (BVG18_07075) secG 315707..316036 (+) 330 WP_004281101.1 preprotein translocase subunit SecG -
  BVG18_RS01485 (BVG18_07105) rimP 317105..317629 (+) 525 WP_004281103.1 ribosome maturation factor RimP -
  BVG18_RS01490 (BVG18_07110) nusA 317664..319148 (+) 1485 WP_004281105.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63552.72 Da        Isoelectric Point: 4.7509

>NTDB_id=213369 BVG18_RS01445 WP_086044367.1 312915..314624(-) (pilB) [Acinetobacter lwoffii strain ZS207]
MTALQGSPRFTGFIRRLVEEGLISAEDMRSALAHAKQEKVDIVAELINQQQLSPTIIAETISVEFGEPLFDISAYDPAQI
LRDAIDEKLITKYRILPIFKNSSILYVATSNPTNIEAIDAVRFSTKLNIETIIVEHNKLEKLIEQNFTEESTFEFDEEFD
LDVDVESADPNKEEDDSNKGEEAPIVKYINKLLIDAIRMGASDLHFEPYEKIYRVRYRVDGVLRQIATPPLQLANRLSSR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKDLFMQALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPQQSLLEMGFT
ETDLQQPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNALEIADASARAGFNNLRRSGLIKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=213369 BVG18_RS01445 WP_086044367.1 312915..314624(-) (pilB) [Acinetobacter lwoffii strain ZS207]
ATGACAGCATTACAGGGGTCGCCAAGATTTACCGGATTTATTCGTCGTTTGGTCGAGGAAGGCCTCATCTCGGCTGAAGA
TATGCGCAGTGCGTTGGCGCATGCCAAACAAGAAAAAGTCGATATTGTGGCTGAGTTAATTAACCAGCAGCAGCTTTCTC
CGACCATCATTGCTGAAACGATTTCAGTTGAATTTGGCGAACCGCTGTTTGATATCAGTGCCTATGATCCTGCCCAAATT
TTGCGGGATGCGATTGATGAAAAGCTGATTACCAAGTATCGCATCCTGCCGATTTTTAAAAATTCCAGTATTTTATATGT
TGCGACCAGCAACCCGACCAATATCGAAGCGATCGATGCAGTCCGTTTTTCGACTAAACTCAATATTGAAACCATTATTG
TTGAACACAATAAACTTGAAAAACTGATCGAGCAGAATTTTACTGAAGAAAGCACGTTTGAGTTTGATGAAGAATTTGAT
CTGGATGTTGATGTCGAATCTGCTGATCCGAATAAAGAAGAGGATGACAGTAACAAGGGTGAAGAAGCACCGATTGTCAA
ATATATTAATAAATTGCTGATTGATGCGATCCGTATGGGCGCTTCAGATCTACACTTTGAACCTTATGAAAAAATCTATC
GGGTACGTTACCGGGTCGATGGTGTTCTACGTCAGATTGCAACCCCGCCCTTACAGCTGGCAAACCGCTTGTCTTCACGT
CTTAAGGTTATGTCGCAAATGGACATTTCAGAAAAACGGGTACCGCAAGATGGTCGTATTAAATTAAAACTTTCTAAAAA
TAAAGCCATTGATTTCCGTGTCAACTCTCTCCCTACCCTGTTTGGTGAGAAGCTGGTACTGCGAATTCTGGATCCCTCCA
GCGCGATGTTGGGAATTGATGCACTAGGTTATGAACCGGAACAAAAAGACCTTTTTATGCAAGCGCTGGACAAACCGCAA
GGCATGCTCCTGATCACTGGTCCAACAGGTTCCGGTAAAACCGTTTCACTGTATACCGGTCTGAATATCCTCAATCGTGA
AGATACCAATATTTCGACAGCGGAAGATCCGGTCGAGATTAACTTACAAGGCATTAATCAAGTCAACGTCAACAATAAAG
TTGGTCTTACCTTCTCTGCCGCACTGAAGTCCTTCTTACGTCAAGACCCGGACATCGTAATGGTCGGTGAGATCCGGGAT
CTGGAAACTGCCGAGATTGCGATTAAAGCGGCGCAGACCGGTCATATGGTGATGTCGACCTTGCATACCAATAGTGCGCC
TGAGACCTTGACCCGTTTACGAAACATGGGGGTGCCCTCTTTCAATATTGCCACTTCAGTAAACCTGGTCATTGCACAAC
GGTTGGCGCGTCGTTTGTGTTCACAATGCAAGAAACCTGCAGATATTCCGCAACAAAGCTTACTGGAGATGGGCTTTACC
GAAACCGACTTGCAGCAACCTGAGTTCCAAATTTATGAACCGGTGGGCTGCAATGAATGTCGTGAAGGCTATAAGGGTCG
TGTAGGTATTTATGAAGTTATGAAAGTAACACCCGAAATTTCCAGAATTATTATGGAGGACGGCAATGCACTTGAAATTG
CAGATGCTTCCGCACGTGCAGGTTTTAACAATTTACGCCGGTCAGGTTTAATCAAGGTGATGCAGGGGGTGACTTCTTTA
CAGGAAGTCAATCGTGTCACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.351

100

0.805
  pilB Acinetobacter baylyi ADP1

77.018

100

0.772
  pilB Legionella pneumophila strain ERS1305867

50.883

99.473

0.506
  pilB Vibrio parahaemolyticus RIMD 2210633

49.195

98.243

0.483
  pilB Vibrio cholerae strain A1552

47.887

99.824

0.478
  pilF Neisseria gonorrhoeae MS11

48.307

98.594

0.476
  pilB Vibrio campbellii strain DS40M4

47.935

97.891

0.469
  pilB/pilB1 Synechocystis sp. PCC 6803

35.237

100

0.38
  pilF Thermus thermophilus HB27

37.699

99.297

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.918

98.067

0.362

Multiple sequence alignment