Detailed information NTG0212708

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: BVG12_RS24365
• Genome accession: NZ_CP019038
• Coordinates: 4983088..4984224 (-)
• Length: 378 a.a.
• NCBI ID: WP_075794643.1
• Uniprot ID: -
• Organism: Massilia putida strain 6NM-7
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4978088..4989224

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BVG12_RS24350	-	4978511..4978993 (-)	483	WP_075794640.1	glutathione peroxidase	-
BVG12_RS24355	-	4979055..4979495 (+)	441	WP_075794641.1	hypothetical protein	-
BVG12_RS24360	-	4979958..4982843 (-)	2886	WP_075794642.1	TonB-dependent receptor	-
BVG12_RS24365	pilU	4983088..4984224 (-)	1137	WP_075794643.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
BVG12_RS24370	pilT	4984244..4985287 (-)	1044	WP_075794644.1	type IV pilus twitching motility protein PilT	Machinery gene
BVG12_RS24375	-	4985351..4986052 (+)	702	WP_075794645.1	YggS family pyridoxal phosphate-dependent enzyme	-
BVG12_RS24380	proC	4986093..4986911 (+)	819	WP_075794646.1	pyrroline-5-carboxylate reductase	-
BVG12_RS24385	-	4986915..4987928 (-)	1014	WP_075794647.1	family 43 glycosylhydrolase	-
BVG12_RS24390	-	4988142..4989023 (+)	882	WP_075794648.1	alpha/beta hydrolase	-

Sequence

Protein

• Length: 378 a.a.
• Molecular weight: 41923.28 Da
• Isoelectric Point: 6.4221

>NTDB_id=212708 BVG12_RS24365 WP_075794643.1 4983088..4984224(-) (pilU) [Massilia putida strain 6NM-7]
MERDQATKFMFDLLRLMVSKGGSDLFITSGFPPAMKIDGRMTPVSSQALSASHTADLARAIMNDKQAAGFELTKEANFAI
SPGDLGRFRVSAFMQMGAVGMVLRVITTTIPKFEDLDLPDVLKDVIMTKRGLVIMVGATGSGKSTTLATMVGYRNENSHG
HIITIEDPVEFVHPHRNCVITQREVGVDTDSFEAALKNSLRQAPDVIQIGEIRDRETMEHAIAFAETGHLCLATLHANSS
NQALDRIINFFPEERRQQLLMDLSLNLKGLISQRLIPKKEAKGRVVAMEILLNSPLISDLIFKGEVHEIKEIMKKSRELG
MQTFDQALFDLYEADKITYEDALRNADSVNDLRLNIKLNSKNAKNRDFSAGTETLGLV

Nucleotide

• Length: 1137 bp

>NTDB_id=212708 BVG12_RS24365 WP_075794643.1 4983088..4984224(-) (pilU) [Massilia putida strain 6NM-7]
ATGGAACGCGACCAGGCCACCAAATTCATGTTCGACCTGCTGCGCCTGATGGTCAGCAAGGGCGGCTCCGACCTGTTCAT
CACGTCAGGCTTTCCGCCGGCGATGAAGATCGACGGCCGCATGACGCCCGTGTCCAGCCAGGCCTTGAGCGCGTCGCACA
CGGCCGACCTGGCGCGCGCCATCATGAACGACAAGCAGGCCGCCGGCTTCGAACTGACGAAGGAAGCCAACTTCGCCATC
AGCCCGGGCGACCTGGGACGCTTCCGCGTCTCCGCCTTCATGCAGATGGGCGCCGTGGGCATGGTGCTGCGCGTGATCAC
GACGACGATCCCGAAGTTCGAAGACCTCGACCTGCCGGACGTGCTGAAGGACGTGATCATGACCAAGCGCGGCCTCGTGA
TCATGGTCGGCGCCACGGGCTCCGGCAAATCGACGACGCTCGCGACGATGGTCGGCTACCGCAACGAGAACAGCCACGGC
CACATCATCACGATCGAGGACCCCGTCGAATTCGTCCACCCGCACAGGAACTGCGTGATCACGCAGCGCGAGGTCGGCGT
CGACACGGACAGCTTCGAGGCGGCGCTGAAGAACTCCCTGCGCCAGGCGCCGGACGTGATCCAGATCGGAGAAATCCGCG
ACCGCGAGACGATGGAACACGCGATCGCGTTCGCCGAGACGGGCCACTTGTGCCTGGCCACCTTGCACGCGAACAGCTCG
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCGGAAGAGCGCCGCCAGCAGCTGCTGATGGACTTGTCGCTGAACCT
GAAGGGCCTGATCTCGCAGCGCCTCATCCCGAAGAAGGAAGCGAAGGGCCGCGTGGTCGCGATGGAGATCCTGCTGAATT
CGCCGCTGATCTCGGACCTGATCTTCAAGGGCGAAGTCCACGAGATCAAGGAGATCATGAAGAAGTCGCGCGAGCTGGGC
ATGCAGACCTTCGACCAGGCCCTGTTCGACCTGTACGAGGCCGACAAGATCACCTACGAGGACGCGCTGCGCAACGCGGA
TTCCGTGAACGATCTGCGCCTGAATATCAAGCTCAACAGCAAGAACGCGAAGAACCGCGACTTCTCGGCGGGGACCGAAA
CGCTCGGTCTCGTCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (118-277)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	65.043	92.328	0.601
pilU	Acinetobacter baylyi ADP1	58.824	94.444	0.556
pilU	Vibrio cholerae strain A1552	56.742	94.18	0.534
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	45.373	88.624	0.402
pilT	Acinetobacter nosocomialis M2	41.692	87.566	0.365
pilT	Pseudomonas aeruginosa PAK	41.194	88.624	0.365