Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Tchl_RS05135 Genome accession   NZ_CP018839
Coordinates   1078104..1079819 (-) Length   571 a.a.
NCBI ID   WP_075147454.1    Uniprot ID   A0A1H5VTP4
Organism   Thauera chlorobenzoica strain 3CB1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1073104..1084819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Tchl_RS05115 (Tchl_1025) - 1074905..1075531 (-) 627 WP_075147450.1 DUF502 domain-containing protein -
  Tchl_RS05120 (Tchl_1026) - 1075581..1075838 (-) 258 WP_075147451.1 FmdB family zinc ribbon protein -
  Tchl_RS05125 (Tchl_1027) pilD 1076005..1076856 (-) 852 WP_075147452.1 A24 family peptidase Machinery gene
  Tchl_RS05130 (Tchl_1028) pilC 1076860..1078089 (-) 1230 WP_075147453.1 type II secretion system F family protein Machinery gene
  Tchl_RS05135 (Tchl_1029) pilB 1078104..1079819 (-) 1716 WP_075147454.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Tchl_RS05140 (Tchl_1030) - 1079908..1081188 (-) 1281 WP_075147455.1 HlyC/CorC family transporter -
  Tchl_RS05145 (Tchl_1031) - 1081385..1082215 (-) 831 WP_075147456.1 inner membrane protein YpjD -
  Tchl_RS05150 (Tchl_1032) ffh 1082265..1083635 (+) 1371 WP_075147457.1 signal recognition particle protein -
  Tchl_RS05155 (Tchl_1033) - 1083652..1084428 (+) 777 WP_075147458.1 EI24 domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 61858.86 Da        Isoelectric Point: 5.8234

>NTDB_id=210883 Tchl_RS05135 WP_075147454.1 1078104..1079819(-) (pilB) [Thauera chlorobenzoica strain 3CB1]
MAASSKPSLSGFARALIQHGHLQEADALACSAHGGEGSNAFIHEVAQRGLMSSAAMAAFAAETFGYPLLDITTFDGSALA
REAVDRKLMQKHQVVPLARRQNRITLAIADPSNIRALDEIRFQTGLQLDLVIAEADKLRHTVDALLESAAETLKELTGEA
FDMDLLHQESAQHQRDEDEASEVDDAPVVRFIQKVLIDAINEGASDVHFEPYEKYYRIRVRTDGILRDIAQPPLLLKDKI
AARIKVISRLDISEKRVPQDGRMKLALSKNKAIDFRVSTLPTLYGEKIVMRILDPGSAMLGIDALGYDPDQKEALLAAID
RPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNISTAEDPAEINLPGINQVNINEKAGLGFSTALRAFLRQDPDIIMVGE
IRDLETAEISVKAAQTGHLVMSTVHTNDAPTTLERLKNMGVAPFNIASSVILITAQRLARRLCACKKPADIPEEALREAG
FSAMELDGSWQPYGPVGCERCKGSGYKGRVGIYQVMPVSDAIAHIIMTGGNSIDIAAQAAREGVRDLRQSGLLKVKQGIT
SLAEVLASTNE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=210883 Tchl_RS05135 WP_075147454.1 1078104..1079819(-) (pilB) [Thauera chlorobenzoica strain 3CB1]
ATGGCGGCAAGCTCCAAACCGTCCCTGAGCGGTTTCGCCAGGGCCCTCATCCAGCACGGGCATCTGCAGGAAGCCGATGC
CCTCGCCTGCTCCGCACATGGCGGCGAAGGCAGCAATGCCTTCATCCACGAAGTCGCCCAGCGCGGGCTGATGAGCAGCG
CGGCGATGGCCGCCTTCGCCGCCGAAACCTTCGGCTACCCCCTGCTCGACATCACGACCTTCGACGGCTCGGCCCTCGCC
CGCGAAGCCGTCGACCGCAAGCTGATGCAGAAGCACCAAGTCGTGCCCCTGGCGCGGCGCCAGAACCGCATCACCCTGGC
GATCGCCGATCCATCGAACATCCGTGCACTCGACGAGATCCGCTTCCAGACCGGTCTCCAGCTCGACCTCGTGATCGCCG
AGGCCGACAAGCTCAGGCACACCGTCGACGCGCTGCTGGAGAGCGCCGCCGAAACGCTCAAGGAGCTCACCGGCGAAGCC
TTCGACATGGATCTGCTGCACCAGGAAAGCGCCCAGCACCAGCGCGACGAGGATGAAGCGTCGGAAGTCGACGACGCGCC
GGTGGTGCGCTTCATCCAGAAAGTGCTGATCGACGCGATCAACGAGGGCGCCTCCGACGTCCACTTCGAGCCCTACGAGA
AGTACTACCGCATCCGCGTGCGCACCGATGGCATCCTGCGCGACATCGCCCAGCCTCCGCTGCTGCTCAAGGACAAGATC
GCCGCCCGCATCAAGGTCATTTCGCGCCTCGACATCTCGGAAAAACGCGTCCCCCAGGACGGGCGGATGAAGCTGGCGCT
GTCGAAGAACAAGGCGATCGACTTCCGCGTCTCCACCCTGCCGACCCTGTACGGGGAAAAGATCGTGATGCGGATCCTCG
ATCCCGGCTCGGCGATGCTCGGCATCGATGCCCTCGGCTACGATCCCGACCAGAAGGAGGCCCTGCTCGCAGCCATCGAT
CGCCCTTACGGCATGATCCTGGTCACCGGCCCCACCGGTTCGGGCAAGACGGTGTCGCTCTATACCTGCCTCAACCTGCT
CAACAAGCCCGGAGTCAATATCTCGACCGCGGAAGATCCGGCCGAAATCAACCTCCCCGGCATCAACCAGGTCAACATCA
ACGAAAAGGCCGGCCTCGGCTTTTCCACAGCGCTGCGCGCCTTCCTCCGCCAGGATCCGGACATCATCATGGTGGGCGAG
ATCCGCGACCTCGAAACCGCCGAGATCTCGGTCAAGGCGGCACAGACCGGCCATCTCGTGATGTCCACGGTGCACACCAA
CGATGCCCCGACCACCCTCGAGCGCCTCAAGAACATGGGGGTCGCGCCGTTCAATATCGCCTCTTCGGTGATCCTGATCA
CCGCCCAGCGCCTTGCCCGCCGCCTGTGCGCCTGCAAGAAACCCGCCGACATCCCGGAGGAGGCACTGCGCGAGGCCGGA
TTCAGCGCCATGGAACTGGACGGCAGCTGGCAGCCCTATGGGCCGGTCGGCTGCGAACGCTGCAAGGGTAGCGGCTACAA
GGGCCGGGTCGGGATCTACCAGGTGATGCCGGTCAGCGACGCAATCGCCCACATCATCATGACCGGCGGCAACTCGATCG
ACATCGCCGCACAGGCCGCGCGCGAGGGCGTTCGCGACCTGCGCCAGTCGGGACTGCTGAAGGTGAAGCAAGGCATCACT
TCGCTCGCCGAAGTGCTGGCCTCGACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1H5VTP4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.435

100

0.548
  pilB Acinetobacter baylyi ADP1

52.622

100

0.527
  pilB Legionella pneumophila strain ERS1305867

52.205

99.299

0.518
  pilF Neisseria gonorrhoeae MS11

53.422

92.119

0.492
  pilB Vibrio cholerae strain A1552

52.174

88.616

0.462
  pilB Vibrio parahaemolyticus RIMD 2210633

48.851

91.419

0.447
  pilB Vibrio campbellii strain DS40M4

48.381

91.944

0.445
  pilF Thermus thermophilus HB27

41.308

93.695

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.265

92.644

0.373

Multiple sequence alignment