Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   Tchl_RS05125 Genome accession   NZ_CP018839
Coordinates   1076005..1076856 (-) Length   283 a.a.
NCBI ID   WP_075147452.1    Uniprot ID   A0A1H5VTM3
Organism   Thauera chlorobenzoica strain 3CB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1071005..1081856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Tchl_RS05105 (Tchl_1023) prmC 1072066..1072929 (+) 864 WP_075147448.1 peptide chain release factor N(5)-glutamine methyltransferase -
  Tchl_RS05110 (Tchl_1024) aspS 1072992..1074803 (-) 1812 WP_075147449.1 aspartate--tRNA ligase -
  Tchl_RS05115 (Tchl_1025) - 1074905..1075531 (-) 627 WP_075147450.1 DUF502 domain-containing protein -
  Tchl_RS05120 (Tchl_1026) - 1075581..1075838 (-) 258 WP_075147451.1 FmdB family zinc ribbon protein -
  Tchl_RS05125 (Tchl_1027) pilD 1076005..1076856 (-) 852 WP_075147452.1 A24 family peptidase Machinery gene
  Tchl_RS05130 (Tchl_1028) pilC 1076860..1078089 (-) 1230 WP_075147453.1 type II secretion system F family protein Machinery gene
  Tchl_RS05135 (Tchl_1029) pilB 1078104..1079819 (-) 1716 WP_075147454.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Tchl_RS05140 (Tchl_1030) - 1079908..1081188 (-) 1281 WP_075147455.1 HlyC/CorC family transporter -

Sequence

Protein


Download         Length: 283 a.a.        Molecular weight: 30541.15 Da        Isoelectric Point: 8.5071

>NTDB_id=210881 Tchl_RS05125 WP_075147452.1 1076005..1076856(-) (pilD) [Thauera chlorobenzoica strain 3CB1]
MAELLHEPLVLVPLLSLLGLFVGSFLNVVIHRLPKMMERDWHAQAAELRGEPAPEAPHFNLATPRSRCPHCGHRLSALEN
IPVLSFVLLRGRCRHCGARISKRYPVVEISTAALSGVAAWHFGASAATLGALLFIWALVALSFIDLDTQLLPDSITLPLL
WLGLAFNTGGVFTDLHSAVIGAIAGYLALWTVYWLFRLATGKEGMGYGDFKLLAAIGAWLGWQVLPLTILLSSLVGAIAG
IALIAFARHGRNVPIPFGPYLGAAGLIALLWGETLTARYLGLF

Nucleotide


Download         Length: 852 bp        

>NTDB_id=210881 Tchl_RS05125 WP_075147452.1 1076005..1076856(-) (pilD) [Thauera chlorobenzoica strain 3CB1]
ATGGCTGAACTCCTCCACGAACCGCTCGTTCTCGTTCCGCTGCTGAGCCTGCTCGGGCTTTTCGTCGGCAGCTTCCTCAA
CGTCGTCATCCATCGCCTGCCGAAGATGATGGAGCGCGACTGGCACGCCCAGGCCGCCGAACTGCGCGGCGAGCCGGCGC
CCGAAGCCCCGCACTTCAACCTCGCCACGCCACGCTCGCGCTGCCCGCACTGCGGCCACCGCCTGTCCGCGCTGGAAAAC
ATCCCGGTGCTCAGCTTCGTCCTCCTGCGCGGGCGCTGCAGGCACTGCGGCGCCCGCATCAGCAAGCGCTATCCGGTGGT
CGAAATATCCACCGCCGCCCTCTCCGGCGTGGCCGCCTGGCATTTCGGCGCCAGCGCCGCCACGCTCGGCGCGCTGCTGT
TCATCTGGGCGCTGGTGGCGCTGAGCTTCATCGATCTCGACACCCAGCTGCTGCCCGACAGCATCACCCTGCCCCTGCTG
TGGCTGGGCCTCGCGTTCAACACCGGCGGCGTATTCACCGACCTGCACAGCGCGGTCATCGGCGCCATCGCCGGCTATCT
CGCGCTGTGGACGGTGTACTGGCTGTTCCGGCTCGCCACCGGCAAGGAAGGCATGGGGTATGGCGACTTCAAGCTGCTGG
CGGCGATCGGCGCCTGGCTCGGCTGGCAGGTGTTGCCGCTCACGATCTTGCTGTCCTCGCTGGTAGGAGCGATCGCCGGC
ATCGCCCTGATCGCCTTCGCGCGCCACGGCCGCAACGTGCCGATTCCGTTCGGCCCTTATCTCGGCGCCGCCGGCCTGAT
CGCCCTGCTGTGGGGCGAGACCCTGACCGCGCGCTACCTGGGCCTGTTCTAG

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-122)

Type IV leader peptidase family (Peptidase_A24)

(133-241)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1H5VTM3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

52.837

99.647

0.527
  pilD Vibrio campbellii strain DS40M4

50.883

100

0.509
  pilD Acinetobacter baumannii D1279779

50.186

95.053

0.477
  pilD Neisseria gonorrhoeae MS11

51.737

91.519

0.473
  pilD Acinetobacter nosocomialis M2

49.442

95.053

0.47

Multiple sequence alignment