Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BJD11_RS06000 Genome accession   NZ_CP018467
Coordinates   830378..832087 (+) Length   569 a.a.
NCBI ID   WP_039416953.1    Uniprot ID   A0AAQ0YN81
Organism   Xanthomonas euvesicatoria strain LMG930     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 825378..837087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJD11_RS05980 (BJD11_05985) coaE 826693..827304 (-) 612 WP_011348341.1 dephospho-CoA kinase -
  BJD11_RS05985 (BJD11_05990) - 827318..828181 (-) 864 WP_008576868.1 A24 family peptidase -
  BJD11_RS05990 (BJD11_05995) pilC 828188..829450 (-) 1263 WP_011348340.1 type II secretion system F family protein Machinery gene
  BJD11_RS05995 (BJD11_06000) pilA 829792..830220 (+) 429 WP_011348339.1 pilin Machinery gene
  BJD11_RS06000 (BJD11_06005) pilB 830378..832087 (+) 1710 WP_039416953.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BJD11_RS06005 (BJD11_06010) pilR 832302..833756 (-) 1455 WP_011348337.1 sigma-54 dependent transcriptional regulator Regulator
  BJD11_RS06010 (BJD11_06020) - 834023..835636 (-) 1614 WP_011348336.1 HAMP domain-containing sensor histidine kinase -
  BJD11_RS06015 (BJD11_06025) sucC 835868..837037 (+) 1170 WP_007965356.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 61684.59 Da        Isoelectric Point: 5.4503

>NTDB_id=208820 BJD11_RS06000 WP_039416953.1 830378..832087(+) (pilB) [Xanthomonas euvesicatoria strain LMG930]
MGITGIARRLVQDGALDEASARTAMDQAAAAKVPLPQWFSEKKLVSASQLAAANAVEFGMPLLDVSAFDASQNAVKLVSE
ELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLGNDDEEMGDL
DVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLNQRIAA
RLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLFLEAIHKP
YGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPDNALLAEGF
TPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALMKAAQGVTSL
AEINRVTKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=208820 BJD11_RS06000 WP_039416953.1 830378..832087(+) (pilB) [Xanthomonas euvesicatoria strain LMG930]
GTGGGAATTACAGGCATTGCACGTCGTTTGGTGCAGGATGGAGCACTGGATGAGGCAAGTGCCCGTACCGCAATGGACCA
AGCCGCGGCGGCGAAGGTTCCGCTTCCCCAATGGTTCTCCGAGAAAAAGCTGGTTTCAGCGTCTCAATTGGCCGCTGCCA
ACGCAGTCGAGTTCGGCATGCCGCTGCTCGATGTTTCTGCATTCGACGCCAGCCAGAACGCAGTAAAGCTCGTCAGTGAG
GAGCTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTTGTTCGTGGGGGTGAGCAATCCGACCCA
GACCCGGGCGCTGGACGACATCAAGTTCCATACGAACTTGGTGGTCGAGGCCATTCTTGTCGACGAGGATCAGATCCGCC
GGACGCTCGAACAGTGGCAGGCCAGCAACGCGTCGTTGGGTTCGTCGCTTGGCAACGACGACGAGGAAATGGGGGATCTG
GACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCCAAGGGCGACGACACGCCGGTGGT
GAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACATCCATTTCGAGCCGTATGAAGACGACT
ACCGGGTGCGCTTGCGCATCGATGGCCTGTTGAAGAACGTGGCCAAGGCGCCGGTGAAGCTGAACCAGCGCATCGCAGCG
CGGTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGACGGGCGCATCAAGCTCAACCTGTCCAA
GACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGAGAGAAGGTGGTGCTGCGTATCCTGGACGGCA
GCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAGCAGAAGCTGTTCCTGGAAGCGATCCACAAGCCC
TATGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTTGTACACGGCGCTGGGCATCCTCAACGA
CGAGACACGCAATATCTCCACCGCGGAGGACCCGGTCGAAATCCGCCTGCCTGGCGTCAATCAGGTACAGCAGAACAACA
AGCGTGGCATGACCTTTGCAGCAGCGCTGCGCTCGTTCCTGCGTCAGGACCCGGACATCATCATGGTCGGCGAAATCCGT
GACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGTGTTGTCGACGTTGCACACCAACGATGC
GCCGCAGACCATCGCACGCTTGATGAACATGGGCATCGCGCCCTACAACATTACCTCGTCGGTGACCTTGGTGATCGCGC
AACGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCTGACAACGCCTTGCTGGCCGAAGGATTC
ACCCCCGCACAGCTTGCTGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGACGAGTGCACCGAAGGCTACAAGGGGCG
TACCGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGCTGGAGGGCGGCAATGCGATGCAGATTG
CCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCCTTGATGAAGGCTGCGCAGGGGGTGACCAGCCTG
GCCGAGATCAATCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-144)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.042

99.824

0.569
  pilB Acinetobacter baylyi ADP1

55.81

99.824

0.557
  pilB Legionella pneumophila strain ERS1305867

53.076

100

0.531
  pilF Neisseria gonorrhoeae MS11

50.176

99.824

0.501
  pilB Vibrio cholerae strain A1552

49.04

100

0.494
  pilB Vibrio parahaemolyticus RIMD 2210633

46.964

98.418

0.462
  pilB Vibrio campbellii strain DS40M4

45.694

100

0.457
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

92.97

0.367
  pilF Thermus thermophilus HB27

39.808

91.388

0.364

Multiple sequence alignment