Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BST98_RS01965 Genome accession   NZ_CP018297
Coordinates   393290..394981 (-) Length   563 a.a.
NCBI ID   WP_109374727.1    Uniprot ID   -
Organism   Photobacterium damselae strain Phdp Wu-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 388290..399981
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BST98_RS01935 (BST98_01930) mutT 388641..389063 (-) 423 WP_109374724.1 8-oxo-dGTP diphosphatase MutT -
  BST98_RS01940 (BST98_01935) yacG 389311..389520 (-) 210 WP_005297592.1 DNA gyrase inhibitor YacG -
  BST98_RS01945 (BST98_01940) zapD 389529..390266 (-) 738 WP_005297594.1 cell division protein ZapD -
  BST98_RS01950 (BST98_01945) coaE 390334..390936 (-) 603 WP_109374725.1 dephospho-CoA kinase -
  BST98_RS01955 (BST98_01950) pilD 390938..391816 (-) 879 WP_109374726.1 prepilin peptidase Machinery gene
  BST98_RS01960 (BST98_01955) pilC 391999..393225 (-) 1227 WP_086367530.1 type II secretion system F family protein Machinery gene
  BST98_RS01965 (BST98_01960) pilB 393290..394981 (-) 1692 WP_109374727.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BST98_RS01970 (BST98_01965) pilA 394981..395376 (-) 396 WP_069530744.1 pilin Machinery gene
  BST98_RS01975 (BST98_01970) ampD 395762..396340 (+) 579 WP_068945541.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BST98_RS01980 (BST98_01975) pdhR 396700..397473 (+) 774 WP_005297609.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62583.14 Da        Isoelectric Point: 5.1729

>NTDB_id=207852 BST98_RS01965 WP_109374727.1 393290..394981(-) (pilB) [Photobacterium damselae strain Phdp Wu-1]
MHTTLASILHQADLISSVQQQQVEDSVSMEGITVPSALIHLGIFSDRELAQHLSHIFGLTLVSVHDYDFEACCKAVYQRD
LVLRHRALPLAQNADTLFLGVSDPCNIDGQDEFRFATGRNIEPLLLENKQLESAIRRIYGRELGETTNMASRDVSDDDLN
NLVDIGDDELDQTTDLGQENEPVTRYINQVLLDAVRKKASDIHFEPYETSYRIRFRCDGILHQHATPPAHLSRRLSTRLK
VMSRLNIAERRQPQDGRIKLKLTANLAVDLRVSTLPTLWGEKVVLRILDSSSANLDIDILGYNQQQKAAYLNALQQPQGM
ILMTGPTGSGKTVSLYTGLSILNTEERNISTAEDPVEINLPGINQVHINNQAGLGFAEALRSFLRQDPDVVMVGEIRDLE
TASIAVKAAQTGHLVLSTLHTNSAAETITRLTNMGIEDFNLASSLTLIIAQRLGRRLCPHCKQVDEIDDFMQDKLRIPAG
TTLYKANQEGCDDCNKGYLGRVGIYEVMPFSRRLAQALVDGATTLELENIAVEEGMLTLQQSGIEKLCLGITSLKELQRV
LHF

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=207852 BST98_RS01965 WP_109374727.1 393290..394981(-) (pilB) [Photobacterium damselae strain Phdp Wu-1]
ATGCACACCACTCTCGCCTCGATTCTGCATCAGGCTGATTTGATCAGCTCGGTACAGCAACAACAAGTCGAAGACAGTGT
CAGTATGGAGGGGATCACGGTTCCCTCCGCACTGATCCATCTGGGCATTTTTAGCGATCGCGAACTGGCCCAACACCTTA
GCCACATATTTGGTCTCACCCTCGTTTCAGTCCATGACTACGATTTTGAAGCTTGCTGTAAAGCGGTCTATCAACGCGAT
CTGGTATTACGCCATCGCGCACTGCCCCTTGCTCAAAATGCGGACACACTGTTTTTAGGGGTCAGCGATCCATGCAACAT
CGACGGGCAAGATGAGTTCCGTTTTGCTACTGGCCGTAATATTGAACCGCTACTGCTGGAAAATAAGCAGCTCGAATCGG
CTATCCGTCGTATTTATGGCCGCGAGTTGGGCGAAACCACCAATATGGCCAGTCGTGATGTCAGCGATGATGATCTAAAT
AACTTAGTCGATATTGGTGATGATGAGCTGGATCAAACCACAGATCTAGGTCAAGAAAACGAACCTGTTACCCGCTATAT
CAACCAAGTGCTGCTCGATGCGGTACGTAAAAAAGCCTCCGATATCCATTTTGAACCTTATGAGACCAGTTATCGGATCC
GCTTTCGTTGTGATGGCATCTTACACCAACACGCAACACCACCAGCCCATCTTTCTCGTCGTCTCTCTACCCGACTCAAA
GTGATGTCGCGCCTAAATATTGCCGAACGACGCCAACCACAAGATGGACGTATAAAACTCAAGCTCACAGCCAATTTAGC
TGTAGATCTGCGGGTTTCTACCCTGCCCACTTTATGGGGTGAAAAAGTGGTGCTGCGTATTTTAGACAGCTCCAGTGCCA
ACTTAGATATTGATATTTTAGGTTACAACCAACAACAAAAAGCCGCTTATCTTAATGCTCTGCAACAACCGCAAGGGATG
ATCTTAATGACAGGGCCGACGGGCAGCGGTAAAACCGTTTCGCTTTATACCGGCCTTAGCATTTTAAATACCGAAGAGCG
CAATATTTCTACCGCAGAAGATCCGGTAGAGATCAACCTACCCGGCATTAATCAGGTTCACATTAACAATCAAGCTGGCC
TCGGATTTGCTGAAGCGCTGCGCTCATTTTTACGTCAAGACCCTGATGTGGTGATGGTAGGTGAAATCCGCGATCTGGAA
ACCGCCTCTATTGCTGTTAAAGCAGCGCAAACTGGACACTTAGTGCTATCGACCTTGCACACCAATTCTGCCGCCGAAAC
CATTACCCGTTTAACCAATATGGGCATTGAAGACTTTAACCTAGCCTCATCACTGACTCTGATTATTGCTCAGCGCTTAG
GTCGCCGGCTCTGCCCTCACTGTAAACAAGTGGATGAGATTGACGACTTTATGCAAGACAAACTGCGCATTCCGGCTGGC
ACTACCTTGTATAAAGCCAATCAAGAAGGGTGTGATGACTGCAATAAAGGTTATTTAGGTCGTGTCGGTATCTATGAAGT
GATGCCATTTAGTCGCCGTCTGGCTCAAGCTCTGGTTGATGGCGCTACTACCTTAGAGCTGGAAAATATCGCAGTAGAAG
AAGGGATGCTGACGCTACAACAATCGGGAATAGAAAAGCTCTGCCTTGGCATCACCAGTTTAAAAGAATTACAGCGCGTC
CTGCATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-143)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

61.989

100

0.62
  pilB Vibrio parahaemolyticus RIMD 2210633

60.924

100

0.609
  pilB Vibrio campbellii strain DS40M4

60.391

100

0.604
  pilB Legionella pneumophila strain ERS1305867

50.551

96.625

0.488
  pilB Acinetobacter baylyi ADP1

48.54

97.336

0.472
  pilB Acinetobacter baumannii D1279779

50.48

92.54

0.467
  pilF Neisseria gonorrhoeae MS11

44.092

100

0.444
  pilB/pilB1 Synechocystis sp. PCC 6803

40.27

92.185

0.371

Multiple sequence alignment