Detailed information NTG0206541

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BSF20_RS14150
• Genome accession: NZ_CP018152
• Coordinates: 2714934..2715548 (+)
• Length: 204 a.a.
• NCBI ID: WP_043867297.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain LM2303
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2709934..2720548

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSF20_RS14115 (BSF20_13825)	aroE	2710621..2711463 (+)	843	WP_025852995.1	shikimate dehydrogenase	-
BSF20_RS14120 (BSF20_13830)	yhbY	2711457..2711747 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BSF20_RS14125 (BSF20_13835)	-	2711758..2712327 (+)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
BSF20_RS14130 (BSF20_13840)	yqeK	2712317..2712898 (+)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BSF20_RS14135 (BSF20_13845)	rsfS	2712895..2713251 (+)	357	WP_003152864.1	ribosome silencing factor	-
BSF20_RS14140 (BSF20_13850)	-	2713248..2713985 (+)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
BSF20_RS14145 (BSF20_13855)	comER	2714054..2714875 (-)	822	WP_044053476.1	late competence protein ComER	-
BSF20_RS14150 (BSF20_13860)	comEA	2714934..2715548 (+)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
BSF20_RS14155 (BSF20_13865)	-	2715615..2716184 (+)	570	WP_003152868.1	ComE operon protein 2	-
BSF20_RS14160 (BSF20_13870)	comEC	2716185..2718536 (+)	2352	WP_072588441.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BSF20_RS14165 (BSF20_13875)	-	2718555..2718689 (-)	135	WP_003152870.1	YqzM family protein	-
BSF20_RS20150	-	2718730..2718884 (+)	155	Protein_2720	hypothetical protein	-
BSF20_RS14170 (BSF20_13880)	holA	2718924..2719965 (+)	1042	Protein_2721	DNA polymerase III subunit delta	-
BSF20_RS14175 (BSF20_13885)	rpsT	2719982..2720248 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22178.37 Da
• Isoelectric Point: 6.9685

>NTDB_id=206541 BSF20_RS14150 WP_043867297.1 2714934..2715548(+) (comEA) [Bacillus amyloliquefaciens strain LM2303]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=206541 BSF20_RS14150 WP_043867297.1 2714934..2715548(+) (comEA) [Bacillus amyloliquefaciens strain LM2303]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363