Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   K04M5_RS13385 Genome accession   NZ_CP017899
Coordinates   2853859..2855082 (+) Length   407 a.a.
NCBI ID   WP_054730505.1    Uniprot ID   A0A1W6UNK4
Organism   Vibrio alginolyticus strain K04M5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2848859..2860082
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K04M5_RS13365 (K04M5_26150) ampD 2849888..2850439 (-) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K04M5_RS13370 (K04M5_26160) nadC 2850532..2851419 (+) 888 WP_005379983.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K04M5_RS13375 (K04M5_26170) pilA 2851682..2852146 (+) 465 WP_033905592.1 pilin Machinery gene
  K04M5_RS13380 (K04M5_26180) pilB 2852150..2853835 (+) 1686 WP_054730502.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K04M5_RS13385 (K04M5_26190) pilC 2853859..2855082 (+) 1224 WP_054730505.1 type II secretion system F family protein Machinery gene
  K04M5_RS13390 (K04M5_26200) pilD 2855147..2856016 (+) 870 WP_086047031.1 prepilin peptidase Machinery gene
  K04M5_RS13395 (K04M5_26210) coaE 2856017..2856631 (+) 615 WP_017635920.1 dephospho-CoA kinase -
  K04M5_RS13400 (K04M5_26220) zapD 2856659..2857399 (+) 741 WP_005373086.1 cell division protein ZapD -
  K04M5_RS13405 (K04M5_26230) yacG 2857558..2857752 (+) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  K04M5_RS13410 (K04M5_26240) rplS 2858407..2858760 (-) 354 WP_005379971.1 50S ribosomal protein L19 -
  K04M5_RS13415 (K04M5_26250) trmD 2858802..2859563 (-) 762 WP_005385420.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45285.44 Da        Isoelectric Point: 10.3992

>NTDB_id=203809 K04M5_RS13385 WP_054730505.1 2853859..2855082(+) (pilC) [Vibrio alginolyticus strain K04M5]
MKSTTPQLKNFRWKGINSSGKKTSGQTLAMTEIEVRERLDAQHIKIKKLKKSSISFLTKLSHRVKGRDITVFTRQISTML
ITGVPLAQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASEHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVVLVALGVSFIMLTKVIPEFEKMFVGFGADLPWFTRQVLDLSAWTQNWSPFIALGSISLFISA
RILSKRSDSFRLMLNRSVLKFPVLGPVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNLHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=203809 K04M5_RS13385 WP_054730505.1 2853859..2855082(+) (pilC) [Vibrio alginolyticus strain K04M5]
ATGAAAAGCACTACACCACAACTTAAAAACTTCCGCTGGAAAGGCATCAACAGTTCTGGCAAAAAGACGTCTGGACAAAC
CCTCGCGATGACAGAAATTGAAGTACGCGAGCGTCTAGACGCACAGCACATCAAGATCAAGAAGTTAAAGAAAAGCAGTA
TTTCGTTTCTCACTAAACTCAGCCATCGCGTAAAAGGCAGAGACATCACGGTGTTTACCCGCCAAATTTCGACGATGTTG
ATAACCGGTGTGCCCTTGGCTCAGGCTTTAAAATTGGTATCAGAGAATCACAAAAAAGCAGAGATGAAATCCATTTTGAT
GAGCGTGACACGCGCCGTTGAAGCGGGGACGCCCATGTCAAAAGCCATGCGCACTGCAAGTGAACACTTTGACCCACTCT
ATACTGATCTTATCGCCACAGGTGAGCAATCCGGTAACTTAGCGGAAGTGTTCGAGCGCTTAGCCACCTACCGAGAAAAA
AATGAACAACTCCGTGCGAAAGTGATCAAAGCACTGATTTACCCAGCGATGGTAGTGCTAGTAGCGTTAGGCGTGTCGTT
TATCATGCTCACCAAAGTCATTCCCGAGTTCGAAAAAATGTTTGTTGGTTTTGGTGCTGACTTACCTTGGTTTACCAGGC
AAGTGTTAGATCTTTCCGCCTGGACACAAAACTGGAGTCCGTTTATCGCACTAGGCTCCATTAGTTTATTCATTTCGGCT
AGAATCCTCTCTAAGCGTTCAGATTCTTTTCGCTTAATGCTCAACCGCTCTGTGCTTAAATTTCCTGTCCTTGGGCCTGT
ATTATCAAAAGCCGCTATCGCCAAATTTAGTCGAACACTCGCCACAAGCTTTACAGCGGGTATTCCAATTCTAACCAGTT
TAAAAACCACATCAAAAACCTCAGGAAACTTACACTATCAACTCGCCATAGAAGAAGTTTACCGTGATACCGCCGCAGGT
ATGCCAATGTATGTTGCCATGCGTAACTGTAATGTGTTCCCTGAATTAGTGTTACAAATGGTCATGATCGGTGAAGAGTC
TGGTCGACTTGACGATATGCTCAATAAAGTTGCAACGATCTACGAGTTTGAAGTCGACAACACCGTCGACAACCTTAGTA
AAATTCTAGAGCCACTAATTATCGTATTTTTAGGTATCGTTGTTGGCGGATTAGTGACAGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1W6UNK4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

86.978

100

0.87
  pilC Vibrio cholerae strain A1552

73.529

100

0.737
  pilC Acinetobacter baylyi ADP1

42.75

98.28

0.42
  pilC Acinetobacter baumannii D1279779

41.191

99.017

0.408
  pilG Neisseria gonorrhoeae MS11

40.494

99.509

0.403
  pilG Neisseria meningitidis 44/76-A

40.247

99.509

0.4
  pilC Legionella pneumophila strain ERS1305867

40.049

100

0.4
  pilC Pseudomonas stutzeri DSM 10701

40.909

97.297

0.398

Multiple sequence alignment